##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527071_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6065247
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16667054119972	33.0	31.0	34.0	31.0	34.0
2	31.39135158057042	31.0	31.0	33.0	30.0	34.0
3	32.39588412475205	33.0	31.0	34.0	31.0	34.0
4	36.32418020238912	37.0	37.0	37.0	35.0	37.0
5	36.43200334627757	37.0	37.0	37.0	35.0	37.0
6	36.24529767707729	37.0	37.0	37.0	35.0	37.0
7	36.41950888397455	37.0	36.0	37.0	35.0	37.0
8	36.590372329436875	37.0	37.0	37.0	35.0	37.0
9	38.720015689385775	39.0	39.0	39.0	39.0	39.0
10	38.162577715301616	39.0	39.0	39.0	37.0	39.0
11	37.86431038999731	39.0	38.0	39.0	35.0	39.0
12	37.20077253242943	39.0	35.0	39.0	35.0	39.0
13	37.08620423867321	39.0	35.0	39.0	34.0	39.0
14	37.68042257800877	40.0	36.0	40.0	33.0	40.0
15	37.63657918630519	40.0	36.0	40.0	33.0	40.0
16	32.63374137937004	39.0	34.0	40.0	3.0	40.0
17	34.187030140734585	39.0	33.0	40.0	17.0	40.0
18	36.266896797442875	39.0	34.0	40.0	30.0	40.0
19	37.1858278813707	39.0	35.0	40.0	32.0	40.0
20	37.45033285536434	39.0	36.0	40.0	33.0	40.0
21	37.674468657253364	40.0	36.0	40.0	33.0	40.0
22	37.749605910525986	40.0	36.0	40.0	34.0	40.0
23	37.75218156820324	40.0	36.0	40.0	34.0	40.0
24	37.76785759920412	40.0	36.0	40.0	34.0	40.0
25	37.72800744965539	40.0	36.0	40.0	34.0	40.0
26	37.64520010479375	40.0	35.0	40.0	34.0	40.0
27	37.510970781569156	40.0	35.0	40.0	34.0	40.0
28	37.41305440652293	40.0	35.0	40.0	34.0	40.0
29	37.20917202547563	40.0	35.0	40.0	33.0	40.0
30	37.164969868498346	39.0	35.0	40.0	33.0	40.0
31	37.12093423400564	39.0	35.0	40.0	33.0	40.0
32	37.07187621542865	39.0	35.0	40.0	33.0	40.0
33	36.721776376131096	39.0	35.0	40.0	32.0	40.0
34	36.33071184075438	39.0	35.0	40.0	30.0	40.0
35	36.35482891298574	39.0	35.0	40.0	31.0	40.0
36	36.43289185090072	38.0	35.0	40.0	31.0	40.0
37	36.25043712152201	38.0	35.0	40.0	31.0	40.0
38	36.25331763075766	38.0	35.0	40.0	31.0	40.0
39	35.90036201328652	38.0	35.0	40.0	30.0	40.0
40	35.84200082865545	38.0	35.0	40.0	30.0	40.0
41	35.745900373059825	38.0	35.0	40.0	30.0	40.0
42	35.7224249894522	38.0	35.0	40.0	30.0	40.0
43	35.65601895520496	38.0	35.0	40.0	30.0	40.0
44	35.867813297628274	38.0	35.0	40.0	30.0	40.0
45	35.9158528910694	38.0	35.0	40.0	30.0	40.0
46	35.844676070075955	38.0	35.0	40.0	31.0	40.0
47	35.54443982248373	38.0	35.0	40.0	30.0	40.0
48	35.51812135598105	37.0	35.0	40.0	30.0	40.0
49	35.47046690761316	37.0	35.0	40.0	30.0	40.0
50	34.978772010439144	37.0	34.0	40.0	28.0	40.0
51	35.06801124504905	36.0	34.0	40.0	28.0	40.0
52	34.78929662716127	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	10.0
15	52.0
16	130.0
17	308.0
18	745.0
19	1443.0
20	2658.0
21	4456.0
22	7241.0
23	11030.0
24	16574.0
25	23522.0
26	32173.0
27	44735.0
28	61409.0
29	84918.0
30	113517.0
31	152184.0
32	211259.0
33	332092.0
34	535118.0
35	537307.0
36	604308.0
37	1026263.0
38	1559564.0
39	702224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.68763019873717	0.2719097837235648	0.0333704464138064	0.007089571125462821
8	99.4988167835539	0.4374760005651872	0.0455711036994866	0.01813611218141652
9	98.80725385132708	0.6646060745753636	0.18357043002535592	0.34456964407220353
10	59.84450427163148	24.402946821456737	5.698877556017092	10.053671350894696
11	38.245796090414785	22.77257628584623	19.100821450470196	19.880806173268788
12	35.797668256544206	14.71547655025426	26.26277215091158	23.22408304228995
13	26.990228097882905	14.901470624362043	28.207029326258272	29.90127195149678
14	23.315241737063637	16.900647244869006	34.36583868719609	25.41827233087127
15	28.088237791469993	18.050608656168496	30.315912938088097	23.545240614273418
16	31.41209253308233	27.08353015136894	23.487073156295203	18.01730415925353
17	31.512550107192666	19.556713848586877	26.16884357718655	22.761892467033906
18	31.212315013716672	20.32962548763472	25.99208243291658	22.46597706573203
19	29.197392950361294	24.138538793226395	24.61139669992005	22.05267155649226
20	30.132029247943244	24.022401725766485	24.61865114479262	21.226917881497652
21	27.74398140751729	25.54323014380123	26.412543462780658	20.30024498590082
22	26.37488629894215	22.013365655182714	30.17285198772614	21.43889605814899
23	29.557015567544077	20.758049919483906	28.336966326350765	21.347968186621255
24	31.945310718590687	20.2957604199796	25.697271685720303	22.061657175709414
25	28.2173998849511	20.00845142827654	24.25370310557839	27.520445581193975
26	24.81674695193782	19.549789151208515	28.751739211939757	26.881724684913905
27	23.074493091542685	18.808698145351705	28.329909729974723	29.786899033130886
28	24.815757709455198	23.379657910057084	25.337072010422656	26.46751237006506
29	29.871215467399757	23.350129021950796	22.8730833220807	23.905572188568744
30	29.006007504723218	19.789713427993945	27.336462966800855	23.867816100481974
31	31.855471013793835	19.542699580083053	26.14482147223353	22.457007933889585
32	34.55750441820424	19.08410325251387	24.75226482944553	21.606127499836365
33	34.684556127722416	19.396357642153735	25.047190988264784	20.87189524185907
34	27.421686206678803	20.448779744666624	32.09181753026711	20.037716518387462
35	25.534607246827708	27.065377551812812	27.971144456276885	19.428870745082598
36	25.50466617435366	28.834390421362887	25.12242287906824	20.538520525215215
37	30.022882827360537	26.973988033793184	22.65960479433072	20.343524344515565
38	27.917840773838233	27.996122828963106	22.766871654196443	21.319164743002222
39	30.933694868485983	23.86756878986132	22.38091869960119	22.817817642051512
40	27.74200292255204	23.403712989759526	22.02657204232573	26.827712045362706
41	23.494657348661978	26.198075692548052	23.334779276095432	26.972487682694542
42	22.224552437847954	23.047041612649906	24.4885162962036	30.23988965329854
43	23.03423092249994	21.235507803721763	25.59913883144413	30.131122442334167
44	24.621140738373885	20.560448733580017	24.71963631489369	30.098774213152407
45	27.95442625832056	21.680914231522642	23.745364368507992	26.61929514164881
46	25.523544218397042	26.20483551617931	23.06216053525932	25.20945973016433
47	23.936634402523097	22.943286563597493	25.66665463088313	27.453424402996284
48	25.243967805433147	21.992294790302854	28.326364944411992	24.437372459852007
49	28.63550322023159	21.788181091388363	25.103116163282387	24.47319952509766
50	24.014125063661876	21.819968749829975	25.754993984581336	28.410912201926813
51	22.741448122393038	22.432969341561854	28.201670929477395	26.623911606567713
52	22.7259829649147	22.75725951474029	26.481196891074678	28.035560629270336
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	10.5
4	20.0
5	58.0
6	96.0
7	1083.5
8	2071.0
9	6648.5
10	11226.0
11	15518.5
12	19811.0
13	19807.5
14	22915.0
15	26026.0
16	27523.0
17	29020.0
18	27322.5
19	25625.0
20	28249.5
21	30874.0
22	34021.0
23	37168.0
24	41772.5
25	46377.0
26	50614.0
27	54851.0
28	58968.5
29	63086.0
30	71138.5
31	79191.0
32	89797.0
33	100403.0
34	101673.5
35	102944.0
36	112042.5
37	121141.0
38	131472.0
39	154656.5
40	167510.0
41	183572.0
42	199634.0
43	208355.5
44	217077.0
45	237143.5
46	257210.0
47	275467.0
48	293724.0
49	327718.5
50	361713.0
51	394297.0
52	426881.0
53	479397.0
54	531913.0
55	540566.5
56	549220.0
57	557126.5
58	565033.0
59	568053.0
60	571073.0
61	452812.5
62	334552.0
63	285251.0
64	189936.0
65	143922.0
66	127402.5
67	110883.0
68	96374.0
69	81865.0
70	66320.5
71	50776.0
72	41594.5
73	32413.0
74	22955.0
75	13497.0
76	9167.0
77	4837.0
78	3756.0
79	2675.0
80	1850.5
81	1026.0
82	622.0
83	218.0
84	132.0
85	46.0
86	35.0
87	24.0
88	21.5
89	18.5
90	18.0
91	9.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	6065247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.371502396390571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.5330547534028	5.737246321000777
2	10.230813057947147	1.7129455206325699
3	4.451659475215526	1.118012338940447
4	2.461781940744863	0.8243525366534662
5	1.4352003834313998	0.6007391724598315
6	0.9355802572669566	0.46993274194356205
7	0.6168819444948803	0.3614960073640277
8	0.4585880030908213	0.3071256453464622
9	0.32218026702356883	0.2427419589711805
>10	4.968178456231098	11.104273601503687
>50	2.9540634580736023	17.80746691831414
>100	2.456097833955232	36.47729638631613
>500	0.10845014286934829	6.131673456276849
>1k	0.0627154270872849	10.09087266985267
>5k	0.002490504324765827	1.4314724845422875
>10k+	0.002264094840696206	5.582352239882
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	105954	1.74690330006346	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	48061	0.7923997159555085	No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	47826	0.7885251828985695	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	19142	0.31560132670606816	No Hit
GAATTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	14837	0.2446231785778881	No Hit
GAATTAGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	12597	0.20769145922663992	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTACTC	11720	0.19323203160563782	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC	11673	0.19245712499425002	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	11557	0.19054458952784611	No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	11156	0.18393315226898427	No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	8884	0.14647383692700397	No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	7726	0.12738145701238549	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTC	7461	0.12301230271413513	No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7272	0.11989618889387357	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	7166	0.11814852717457343	No Hit
GAATTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	6899	0.11374639812690233	No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAAAGCACTGACTGCTC	6534	0.10772850635761412	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	6472	0.10670628912557065	No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	6191	0.10207333683195424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.006891722628938278	0.0	0.0	0.0	0.0
7	0.007007134251910928	0.0	0.0	0.0	0.0
8	0.007056596376042064	0.0	0.0	0.0	0.0
9	0.0075841923667741805	0.0	0.0	0.0	0.0
10	0.012761228025833078	0.0	0.0	0.0	0.0
11	0.024186978700125487	0.0	0.0	0.0	0.0
12	0.029660787103971197	0.0	0.0	0.0	0.0
13	0.036189787489281144	0.0	0.0	0.0	0.0
14	0.052776086447922074	0.0	0.0	0.0	0.0
15	0.06347639263495782	0.0	0.0	0.0	0.0
16	0.08157953006695358	0.0	0.0	0.0	0.0
17	0.09046622503584767	0.0	0.0	0.0	0.0
18	0.09899019776111344	0.0	0.0	0.0	0.0
19	0.11422453199350331	0.0	0.0	0.0	0.0
20	0.12377072195081255	0.0	0.0	0.0	0.0
21	0.134240204891903	0.0	0.0	0.0	0.0
22	0.14424804134110283	0.0	0.0	0.0	0.0
23	0.1529368878134724	0.0	0.0	0.0	0.0
24	0.161279499416924	0.0	0.0	0.0	0.0
25	0.1674457775586056	0.0	0.0	0.0	0.0
26	0.17364503044970797	0.0	0.0	0.0	0.0
27	0.1808994753222746	0.0	0.0	0.0	0.0
28	0.1885331298131799	0.0	0.0	0.0	0.0
29	0.19451804683304735	0.0	0.0	0.0	0.0
30	0.20060188810117707	0.0	0.0	0.0	0.0
31	0.2063065197509681	0.0	0.0	0.0	0.0
32	0.21262118426504312	0.0	0.0	0.0	0.0
33	0.2172046744345284	0.0	0.0	0.0	0.0
34	0.22373367481983833	0.0	0.0	0.0	0.0
35	0.23583540785725626	0.0	0.0	0.0	0.0
36	0.2485636611336686	0.0	0.0	0.0	0.0
37	0.25616434087515316	0.0	0.0	0.0	0.0
38	0.26310552562822254	0.0	0.0	0.0	0.0
39	0.27007968513071273	0.0	0.0	0.0	0.0
40	0.2777298269963284	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATTAG	643675	0.0	43.217167	1
AATTAGG	640885	0.0	43.198235	2
ATTAGGG	634010	0.0	43.194336	3
TAGGGGG	165295	0.0	43.072388	5
TTAGGGG	380035	0.0	43.065952	4
TAGGGAG	54850	0.0	42.976665	5
TTAGGGC	62090	0.0	42.873573	4
TTAGGGA	159305	0.0	42.832367	4
TAGGGGC	81610	0.0	42.70261	5
TAGGGGA	78995	0.0	42.59928	5
TAGGGGT	56345	0.0	42.538467	5
TTAGGGT	36125	0.0	42.4537	4
TAGGGAT	44510	0.0	42.325993	5
TAGGGAA	32375	0.0	42.312897	5
TAGGGCA	29260	0.0	42.29768	5
AGGGGGG	105415	0.0	42.279938	6
TAGGGTG	15890	0.0	42.22215	5
AGGGATC	12725	0.0	42.0778	6
TAGGGAC	29135	0.0	41.610775	5
TAGGGCC	7730	0.0	41.447605	5
>>END_MODULE