FastQCFastQC Report
Fri 17 Jun 2016
SRR1527070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5877283
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC992991.6895391969384492No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT471980.803058147786996No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA463970.7894294013066923No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC190960.32491203843680827No Hit
GAATTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT147620.2511704813261502No Hit
GAATTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC112080.1907003627356382No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGCACCCGACTACTC110900.18869263229284688No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTC110590.1881651776849949No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC104170.1772417629030285No Hit
GAATTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC83020.1412557469157092No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT73740.12546613800968917No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAGGAGCACCCGACTGCTC73410.12490465407229837No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC69550.11833699347130297No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG69120.117605362886218No Hit
GAATTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG64920.1104592036830624No Hit
GAATTAGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAAAGCACTGACTGCTC64760.11018696904675171No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC63020.10722641737687295No Hit
GAATTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC60330.10264947255389947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC206.313585E-446.026
GAATTAG6229950.043.266931
AATTAGG6203250.043.2651752
ATTAGGG6133600.043.2247473
TTAGGGG3670300.043.1524964
TAGGGGC782850.043.073775
TTAGGGC617050.043.0106124
TAGGGGG1611600.042.975865
TAGGGAG518250.042.9688345
TTAGGGA1520300.042.9183044
TAGGGGA754200.042.8833245
TAGGGAT426850.042.7508475
TAGGGGT539700.042.727075
TTAGGGT352500.042.4178734
TAGGGCA291750.042.2868925
AGGGGGG1023850.042.0889786
TAGGGAC282700.041.956495
TAGGGCT182300.041.8869975
TAGGGAA307700.041.8290565
TAGGGTG156750.041.818185