FastQCFastQC Report
Fri 17 Jun 2016
SRR1527069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153638
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC43530.37732806998382507No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG41840.3626787605817423No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT38750.3358939285980524No Hit
AGTCGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA29800.25831326637992164No Hit
AGTCGTGGGACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTT25270.21904618259800732No Hit
AGTCGTGGGGACATGCCCAGATATTTCTCTAGATCTTAACATTTTCCCCA24000.20803752997040664No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC20810.18038587494517344No Hit
AGTCGTGGGCGGTAGATGCTTAATAATGTGTATTGAATGACTATCTCATG18420.1596688042522871No Hit
AGTCGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA15730.1363512644347707No Hit
AGTCGTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG15480.13418420683091228No Hit
AGTCGTGGGAGGCAAAATTTGTTCCTGAAGTTGCTTTTTACCCCAGCAGA15160.13141037309797354No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC14780.12811644554010876No Hit
AGTCGTGGGTGTGCATGAGTGTGTGCGTGTATGTGTGTGTATGTGTGTGT14690.12733630480271974No Hit
AGTCGTGGGGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCT13740.11910148590805782No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA13010.11277367770479128No Hit
AGTCGTGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAG12810.11104003162170455No Hit
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12760.11060662010093288No Hit
AGTCGTGGGATCGGAGGGGCCTTTCTTCAAGTAATAGGCATTCCATTTGT12310.10670591641398774No Hit
AGTCGTGGGGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCGCGCCGTC11640.10089820203564723No Hit
AGTCGTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC11570.10029142590656688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG207.8564923E-444.00000414
AAGCGAC408.312782E-944.00000429
TTATCGA408.312782E-944.00000436
CCCGTTA207.8564923E-444.00000417
ACGATAT207.8564923E-444.00000433
TCAATCG207.8564923E-444.00000430
CCGTACG207.8564923E-444.00000438
TCGGAAC207.8564923E-444.00000427
TCGTCTA207.8564923E-444.00000441
ACTACGA207.8564923E-444.00000430
TCGTAAC207.8564923E-444.00000431
TCTCACG207.8564923E-444.00000427
ATAACGG207.8564923E-444.00000417
CGATATG207.8564923E-444.00000434
CGATATA408.312782E-944.00000431
CGATAAC207.8564923E-444.00000426
TACACGT207.8564923E-444.00000438
ACGACCA207.8564923E-444.00000433
TACGTCA207.8564923E-444.00000420
CTTACGT207.8564923E-444.00000418
Produced by FastQC (version 0.11.5)