##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527069_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1153638 Sequences flagged as poor quality 0 Sequence length 50 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80214417347556 31.0 31.0 33.0 31.0 34.0 2 32.53265582444406 34.0 31.0 34.0 31.0 34.0 3 31.05882087795305 31.0 31.0 33.0 30.0 33.0 4 35.91059240420305 37.0 35.0 37.0 35.0 37.0 5 36.17120188482002 37.0 35.0 37.0 35.0 37.0 6 35.426567086035654 35.0 35.0 37.0 35.0 37.0 7 36.301978610274624 37.0 35.0 37.0 35.0 37.0 8 36.63229106530818 37.0 37.0 37.0 35.0 37.0 9 38.73739595956444 39.0 39.0 39.0 39.0 39.0 10 38.10859992475976 39.0 38.0 39.0 37.0 39.0 11 37.75181816132964 39.0 37.0 39.0 35.0 39.0 12 37.29909382319237 39.0 37.0 39.0 35.0 39.0 13 37.19055457604552 39.0 37.0 39.0 34.0 39.0 14 38.074933384649256 40.0 38.0 40.0 34.0 40.0 15 38.014555692513596 40.0 38.0 40.0 34.0 40.0 16 37.83518833464223 40.0 38.0 40.0 33.0 40.0 17 37.89259455739149 40.0 38.0 40.0 33.0 40.0 18 37.79744859305952 40.0 37.0 40.0 33.0 40.0 19 37.738791544661325 40.0 37.0 40.0 33.0 40.0 20 37.50715909150011 40.0 37.0 40.0 33.0 40.0 21 37.779277381639645 40.0 37.0 40.0 33.0 40.0 22 37.611209928937846 40.0 37.0 40.0 33.0 40.0 23 37.717322071568375 40.0 37.0 40.0 33.0 40.0 24 37.74318547065891 40.0 37.0 40.0 34.0 40.0 25 37.72172206532725 40.0 37.0 40.0 34.0 40.0 26 37.61250409573887 39.0 37.0 40.0 34.0 40.0 27 37.49013121967203 39.0 37.0 40.0 33.0 40.0 28 37.31106464939609 39.0 36.0 40.0 33.0 40.0 29 37.624717632394216 40.0 37.0 40.0 33.0 40.0 30 37.7216813246443 40.0 37.0 40.0 34.0 40.0 31 37.67746468129517 40.0 37.0 40.0 34.0 40.0 32 36.98939095279455 40.0 36.0 40.0 32.0 40.0 33 37.22706949667053 40.0 36.0 40.0 33.0 40.0 34 37.172426705777724 40.0 36.0 40.0 33.0 40.0 35 37.19096284969809 40.0 36.0 40.0 33.0 40.0 36 37.01724024347325 40.0 35.0 40.0 32.0 40.0 37 37.06534978910195 40.0 35.0 40.0 32.0 40.0 38 36.867542504667846 40.0 35.0 40.0 32.0 40.0 39 36.866147786394 40.0 35.0 40.0 32.0 40.0 40 36.803112414812965 39.0 35.0 40.0 32.0 40.0 41 36.66161742244967 39.0 35.0 40.0 31.0 40.0 42 36.57357940705837 39.0 35.0 40.0 31.0 40.0 43 36.457098327204896 39.0 35.0 40.0 31.0 40.0 44 36.31735345056248 39.0 35.0 40.0 31.0 40.0 45 36.23226350033546 39.0 35.0 40.0 31.0 40.0 46 35.93665690623922 39.0 35.0 40.0 30.0 40.0 47 35.876445644127536 38.0 35.0 40.0 30.0 40.0 48 35.792019680350336 38.0 35.0 40.0 30.0 40.0 49 35.48610482664406 38.0 35.0 40.0 29.0 40.0 50 34.86120429458808 37.0 34.0 40.0 27.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 0.0 14 2.0 15 11.0 16 32.0 17 84.0 18 147.0 19 263.0 20 461.0 21 725.0 22 1072.0 23 1753.0 24 2670.0 25 3981.0 26 5285.0 27 7243.0 28 9569.0 29 12733.0 30 17350.0 31 23078.0 32 30763.0 33 42818.0 34 71264.0 35 73836.0 36 115792.0 37 186459.0 38 432229.0 39 114016.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.33800724317334 0.5988880394023082 0.03545306239912347 0.02765165502523322 8 99.07353953319846 0.6492504581159775 0.23248193974192946 0.04472806894363743 9 98.01722897477372 1.2588871032334232 0.39821850528502006 0.3256654167078407 10 48.12549517266248 38.775595117359174 4.679370825163526 8.41953888481482 11 30.763723108982195 24.066301560801566 25.57379351234963 19.596181817866608 12 28.179896986749743 16.983837217567384 29.7113132542444 25.12495254143848 13 24.41901185640556 17.20262335325293 31.788481308694756 26.589883481646755 14 26.225037663461155 19.9278283135611 30.795188785390216 23.051945237587525 15 24.4977193885777 22.011237493910567 29.58484377248322 23.906199345028508 16 29.415466550165647 21.820536424771028 26.101862109257844 22.662134915805478 17 28.000031205629494 21.289693994129873 25.19386497324117 25.51640982699946 18 26.20154675903533 21.245919430531934 27.31688796658917 25.235645843843564 19 26.824792525905007 21.776155085044007 26.78552544212309 24.61352694692789 20 25.828726168867533 22.522142994596226 26.705777722301104 24.94335311423514 21 26.83805491844062 21.5960292570113 28.530093495533258 23.03582232901482 22 25.8790885875812 21.84116681315976 28.715853673336007 23.563890925923037 23 25.936125543714756 22.99924239666169 28.225665243343233 22.838966816280323 24 25.600318297420856 22.710503641523598 26.408544101355886 25.28063395969966 25 26.06822937524596 24.086065126148757 25.327702450855465 24.518003047749815 26 26.354974437388506 23.706396633952764 26.38548660845083 23.553142320207897 27 25.989695207682136 20.34589706649746 26.8576451191795 26.806762606640905 28 25.876141389239955 23.099100411047484 25.713698751254725 25.311059448457833 29 25.86253226748772 23.977018787522603 25.907347018735514 24.253101926254164 30 26.09978173395814 23.059486598048956 26.16245303986172 24.67827862813118 31 27.464507930564007 23.329328610881404 25.19507852549933 24.01108493305526 32 27.640733054909774 23.576459860025416 25.531925959443086 23.250881125621728 33 28.676933318770708 22.408242446937425 26.645880250130453 22.26894398416141 34 26.3229019848514 23.91252715323178 27.122979652195923 22.6415912097209 35 26.064935447688097 24.882328772110487 27.211135555520883 21.841600224680533 36 25.198805864577974 26.606959895565158 26.155518455529375 22.038715784327493 37 26.368756923749043 26.308772769274242 25.416898541830278 21.905571765146433 38 25.82369859522658 27.09576140869146 25.209034376468182 21.871505619613778 39 26.655848715108206 24.478389234751283 25.37607117657359 23.489690873566925 40 25.126166093696632 26.311026509182255 25.023794292490365 23.539013104630744 41 24.050438699141324 26.02341462399817 25.29259611767296 24.633550559187544 42 23.63887111901654 25.459286188561748 25.767095050613797 25.134747641807913 43 24.571312664804733 23.688973490817745 26.306432347062074 25.433281497315445 44 23.54759465274202 23.87221988180001 25.650767398438678 26.929418067019288 45 25.7101447767844 25.325882122468222 25.158411910842048 23.805561189905326 46 25.253068986978583 24.550422229503535 25.6351645836909 24.56134419982698 47 24.46738058212368 24.356947326631058 26.102122156170303 25.073549935074958 48 24.878774797640162 25.680586111067772 24.931044227045227 24.509594864246846 49 25.33481039979612 24.566198408859623 25.56841920949206 24.53057198185219 50 23.95439470613832 24.60087133052136 25.952421816895765 25.49231214644455 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 2.0 9 76.5 10 151.0 11 714.5 12 1278.0 13 2705.5 14 4133.0 15 5097.5 16 6062.0 17 5334.0 18 4606.0 19 4564.0 20 4522.0 21 4980.5 22 5439.0 23 5526.5 24 5614.0 25 6628.0 26 7642.0 27 7723.5 28 7805.0 29 10357.0 30 12909.0 31 15696.5 32 18484.0 33 19380.0 34 20276.0 35 21300.5 36 22325.0 37 26741.0 38 31157.0 39 32193.5 40 33230.0 41 35762.0 42 38294.0 43 41699.5 44 45105.0 45 48185.5 46 51266.0 47 51587.0 48 51908.0 49 57910.0 50 63912.0 51 67043.5 52 70175.0 53 68497.5 54 66820.0 55 71492.0 56 76164.0 57 83125.5 58 90087.0 59 90624.5 60 91162.0 61 87050.5 62 82939.0 63 81045.0 64 79151.0 65 65664.0 66 52177.0 67 43590.0 68 35003.0 69 27243.5 70 19484.0 71 19695.0 72 19906.0 73 17200.0 74 14494.0 75 12386.0 76 10278.0 77 8584.0 78 6890.0 79 4150.5 80 1411.0 81 1087.5 82 764.0 83 588.0 84 412.0 85 275.0 86 138.0 87 93.5 88 49.0 89 29.0 90 9.0 91 5.0 92 1.0 93 2.0 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1153638.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.925526312892439 #Duplication Level Percentage of deduplicated Percentage of total 1 69.68067880310153 6.916174109603906 2 11.230145079851171 2.229302009753247 3 4.524710419073501 1.3473039696819784 4 2.2110778213405498 0.8778444438227407 5 1.2053669781477858 0.5981950829148747 6 0.7504105709180895 0.44689319202720806 7 0.49820169817279775 0.346143984503926 8 0.34688208108771174 0.27543897786455773 9 0.2672406871078987 0.23872540245884138 >10 3.8528671169213498 10.600794847801392 >50 2.7104575034569636 19.463321072262932 >100 2.5892258097012975 45.23187318408656 >500 0.10530344202134463 6.812456610038538 >1k 0.027431989097997343 4.615533113179288 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 4353 0.37732806998382507 No Hit AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 4184 0.3626787605817423 No Hit AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 3875 0.3358939285980524 No Hit AGTCGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA 2980 0.25831326637992164 No Hit AGTCGTGGGACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTT 2527 0.21904618259800732 No Hit AGTCGTGGGGACATGCCCAGATATTTCTCTAGATCTTAACATTTTCCCCA 2400 0.20803752997040664 No Hit AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 2081 0.18038587494517344 No Hit AGTCGTGGGCGGTAGATGCTTAATAATGTGTATTGAATGACTATCTCATG 1842 0.1596688042522871 No Hit AGTCGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA 1573 0.1363512644347707 No Hit AGTCGTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG 1548 0.13418420683091228 No Hit AGTCGTGGGAGGCAAAATTTGTTCCTGAAGTTGCTTTTTACCCCAGCAGA 1516 0.13141037309797354 No Hit AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC 1478 0.12811644554010876 No Hit AGTCGTGGGTGTGCATGAGTGTGTGCGTGTATGTGTGTGTATGTGTGTGT 1469 0.12733630480271974 No Hit AGTCGTGGGGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCT 1374 0.11910148590805782 No Hit AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA 1301 0.11277367770479128 No Hit AGTCGTGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAG 1281 0.11104003162170455 No Hit AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1276 0.11060662010093288 No Hit AGTCGTGGGATCGGAGGGGCCTTTCTTCAAGTAATAGGCATTCCATTTGT 1231 0.10670591641398774 No Hit AGTCGTGGGGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCGCGCCGTC 1164 0.10089820203564723 No Hit AGTCGTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC 1157 0.10029142590656688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.668230415433611E-5 0.0 0.0 0.0 0.0 8 5.200938249260166E-4 0.0 0.0 0.0 0.0 9 5.200938249260166E-4 0.0 0.0 0.0 0.0 10 0.007021266636501225 0.0 0.0 0.0 0.0 11 0.012742298710687407 0.0 0.0 0.0 0.0 12 0.01603622626855218 0.0 0.0 0.0 0.0 13 0.021757258342738364 0.0 0.0 0.0 0.0 14 0.031899087928795686 0.0 0.0 0.0 0.0 15 0.04082736525669231 0.0 0.0 0.0 0.0 16 0.05504326313800343 0.0 0.0 0.0 0.0 17 0.059897472170646254 0.0 0.0 0.0 0.0 18 0.06535845733236943 0.0 0.0 0.0 0.0 19 0.07480682848519206 0.0 0.0 0.0 0.0 20 0.08104795438430426 0.0 0.0 0.0 0.0 21 0.08980286710389221 0.0 0.0 0.0 0.0 22 0.09977133208164086 0.0 0.0 0.0 0.0 23 0.10713932793475943 0.0 0.0 0.0 0.0 24 0.1185813920831318 0.0 0.0 0.0 0.0 25 0.12343560111577462 0.0 0.0 0.0 0.0 26 0.13002345623150416 0.0 0.0 0.0 0.0 27 0.13609121752230768 0.0 0.0 0.0 0.0 28 0.1438059425920436 0.0 0.0 0.0 0.0 29 0.14866015162468643 0.0 0.0 0.0 0.0 30 0.1543811836988726 0.0 0.0 0.0 0.0 31 0.1596688042522871 0.0 0.0 0.0 0.0 32 0.1653898363264733 0.0 0.0 0.0 0.0 33 0.17076413918404212 0.0 0.0 0.0 0.0 34 0.177178629691463 0.0 0.0 0.0 0.0 35 0.18489335476119892 0.0 0.0 0.0 0.0 36 0.20084289872559677 0.0 0.0 0.0 0.0 37 0.20734407153717196 0.0 0.0 0.0 0.0 38 0.21315178591551248 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTCG 20 7.8564923E-4 44.000004 14 AAGCGAC 40 8.312782E-9 44.000004 29 TTATCGA 40 8.312782E-9 44.000004 36 CCCGTTA 20 7.8564923E-4 44.000004 17 ACGATAT 20 7.8564923E-4 44.000004 33 TCAATCG 20 7.8564923E-4 44.000004 30 CCGTACG 20 7.8564923E-4 44.000004 38 TCGGAAC 20 7.8564923E-4 44.000004 27 TCGTCTA 20 7.8564923E-4 44.000004 41 ACTACGA 20 7.8564923E-4 44.000004 30 TCGTAAC 20 7.8564923E-4 44.000004 31 TCTCACG 20 7.8564923E-4 44.000004 27 ATAACGG 20 7.8564923E-4 44.000004 17 CGATATG 20 7.8564923E-4 44.000004 34 CGATATA 40 8.312782E-9 44.000004 31 CGATAAC 20 7.8564923E-4 44.000004 26 TACACGT 20 7.8564923E-4 44.000004 38 ACGACCA 20 7.8564923E-4 44.000004 33 TACGTCA 20 7.8564923E-4 44.000004 20 CTTACGT 20 7.8564923E-4 44.000004 18 >>END_MODULE