FastQCFastQC Report
Fri 17 Jun 2016
SRR1527068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227677
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC42930.349684811232922No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC39500.3217458663801635No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT39320.3202796826852666No Hit
AGTCGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG28180.2295392029010888No Hit
AGTCGTGGGACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTTTT26910.21919446238709367No Hit
AGTCGTGGGGACATGCCCAGATATTTCTCTAGATCTTAACATTTTCCCCAGC23430.1908482442857527No Hit
AGTCGTGGGCGGTAGATGCTTAATAATGTGTATTGAATGACTATCTCATGGA19930.1623391168849787No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT19230.1566372914048239No Hit
AGTCGTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC16420.13374853483448823No Hit
AGTCGTGGGAGGCAAAATTTGTTCCTGAAGTTGCTTTTTACCCCAGCAGACA16050.13073471279497784No Hit
AGTCGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA14590.11884233393636925No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT14390.11721324094203932No Hit
AGTCGTGGGGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGA13000.1058910446314462No Hit
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12290.10010776450157494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC351.0196527E-746.00000420
GGTCGTA351.0196527E-746.0000049
CATGACG351.0196527E-746.00000433
GACGTAT351.0196527E-746.0000049
AACGGTA351.0196527E-746.00000436
GGACGTA551.8189894E-1246.0000048
TCGCGTT351.0196527E-746.00000421
GATACGT206.311624E-446.034
CTCACGA301.8615083E-646.044
CGAACCG453.092282E-1046.013
CGTATTA301.8615083E-646.011
CGTATCG301.8615083E-646.037
TTTACGC206.311624E-446.013
CGACGAA206.311624E-446.037
TTATGCG253.417293E-546.023
CCGGATT405.6115823E-946.021
CCTACGC206.311624E-446.038
CACGACC206.311624E-446.032
TAACGGT206.311624E-446.040
TAACGAA206.311624E-446.031
Produced by FastQC (version 0.11.5)