##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1227677
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72905902774101	31.0	31.0	33.0	31.0	34.0
2	32.43730150520047	34.0	31.0	34.0	31.0	34.0
3	30.9874152566188	31.0	31.0	33.0	30.0	33.0
4	35.83548034214211	37.0	35.0	37.0	35.0	37.0
5	36.09859352256335	37.0	35.0	37.0	35.0	37.0
6	35.136645876724906	35.0	35.0	37.0	33.0	37.0
7	36.23405504868137	37.0	35.0	37.0	35.0	37.0
8	36.59728332452266	37.0	37.0	37.0	35.0	37.0
9	38.707009254062754	39.0	39.0	39.0	39.0	39.0
10	38.05801200152809	39.0	38.0	39.0	37.0	39.0
11	37.763161645937814	39.0	37.0	39.0	35.0	39.0
12	37.37614698328632	39.0	37.0	39.0	35.0	39.0
13	37.3177138612192	39.0	37.0	39.0	35.0	39.0
14	38.20509873525366	40.0	38.0	40.0	35.0	40.0
15	38.06141680588624	40.0	38.0	40.0	34.0	40.0
16	32.98734602016654	40.0	35.0	40.0	3.0	40.0
17	34.683311652820734	39.0	35.0	40.0	17.0	40.0
18	36.673300876370575	39.0	34.0	40.0	30.0	40.0
19	37.5119669098631	39.0	37.0	40.0	33.0	40.0
20	37.79216194487638	39.0	37.0	40.0	34.0	40.0
21	37.997914760967255	40.0	38.0	40.0	34.0	40.0
22	37.99825849958906	40.0	38.0	40.0	34.0	40.0
23	38.00300730566753	40.0	38.0	40.0	34.0	40.0
24	37.951484796082354	40.0	38.0	40.0	34.0	40.0
25	37.90185529255659	40.0	37.0	40.0	34.0	40.0
26	37.803015776951106	40.0	37.0	40.0	34.0	40.0
27	37.70570923785328	40.0	37.0	40.0	34.0	40.0
28	37.582256570742956	40.0	37.0	40.0	33.0	40.0
29	37.35002040438975	39.0	36.0	40.0	33.0	40.0
30	37.40162844135713	39.0	36.0	40.0	33.0	40.0
31	37.33034177556475	39.0	36.0	40.0	33.0	40.0
32	37.2689559224454	39.0	36.0	40.0	33.0	40.0
33	36.966347826016126	39.0	35.0	40.0	32.0	40.0
34	36.82355619597011	39.0	35.0	40.0	31.0	40.0
35	36.7652924995744	39.0	35.0	40.0	31.0	40.0
36	36.70225311706581	39.0	35.0	40.0	31.0	40.0
37	36.519483544938936	39.0	35.0	40.0	31.0	40.0
38	36.479694577645425	38.0	35.0	40.0	31.0	40.0
39	36.12109455500103	38.0	35.0	40.0	30.0	40.0
40	36.15065770556914	38.0	35.0	40.0	30.0	40.0
41	36.07079142152211	38.0	35.0	40.0	30.0	40.0
42	36.03005513665239	38.0	35.0	40.0	30.0	40.0
43	35.9038346405447	38.0	35.0	40.0	30.0	40.0
44	36.23508789363978	38.0	35.0	40.0	31.0	40.0
45	36.32510587068097	39.0	35.0	40.0	31.0	40.0
46	36.26576290017651	39.0	35.0	40.0	31.0	40.0
47	35.97146399256482	38.0	35.0	40.0	31.0	40.0
48	35.96134569597704	38.0	35.0	40.0	31.0	40.0
49	35.883928753246984	38.0	35.0	40.0	30.0	40.0
50	35.36847965710851	37.0	35.0	40.0	29.0	40.0
51	35.45089139895917	37.0	34.0	40.0	29.0	40.0
52	34.99470789140792	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	9.0
16	30.0
17	60.0
18	100.0
19	206.0
20	351.0
21	633.0
22	1140.0
23	1863.0
24	2764.0
25	4216.0
26	5998.0
27	8199.0
28	10907.0
29	15019.0
30	20940.0
31	27460.0
32	37048.0
33	52708.0
34	83279.0
35	98625.0
36	158320.0
37	238989.0
38	373007.0
39	85802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.36693446240339	0.5806087431791913	0.03298913313518132	0.019467661282242806
8	99.0673442607461	0.6403964560711002	0.24387522125119226	0.048384061931599276
9	98.04020112782108	1.240309951233101	0.38837576984825817	0.3311131510975607
10	48.43602999811839	38.4662252367683	4.621003733066596	8.476741032046704
11	30.67940508782033	24.21956263740381	25.427046364801164	19.673985909974693
12	28.348173012934186	16.997386120290596	29.591578240856514	25.062862625918708
13	24.44307419622588	17.17870417055952	31.70451185450245	26.67370977871215
14	26.28012091128204	19.937410247157842	30.808429252971266	22.974039588588855
15	24.589936929664724	21.901363306472305	29.792689771006543	23.716009992856428
16	27.904570990578144	29.36717068088756	22.94944028437447	19.778818044159824
17	28.056972640197706	21.20899878388208	25.143014001239738	25.591014574680475
18	26.402221431207067	21.122412491233444	27.286900381777947	25.18846569578155
19	26.870259848478064	21.620018946351525	26.869526756630613	24.640194448539805
20	25.76532752507378	22.606027481169722	26.763880075948315	24.864764917808184
21	26.97704689425639	21.55241158708683	28.493895381276996	22.976646137379785
22	25.658866297894317	21.664656094396165	28.824193985877393	23.852283621832125
23	25.92766664195876	22.94324973099602	28.293354033674984	22.835729593370242
24	25.65707429560055	22.69880432719681	26.311073678174306	25.333047699028327
25	26.068094458069996	24.075632271354763	25.26185633517611	24.59441693539913
26	26.356362463416684	23.641071715117253	26.365648293484362	23.63691752798171
27	26.03803769232461	20.16703090470865	26.851851097642133	26.943080305324607
28	26.074203556798736	22.903499861934367	25.651291015470683	25.371005565796217
29	26.08430393336358	23.867922914577694	25.671165950001505	24.37660720205722
30	26.23108521215271	22.98014868731759	25.995029637274296	24.7937364632554
31	27.513262853340088	23.270941786805487	25.27065343734549	23.945141922508935
32	27.747200607325865	23.25041521507693	25.525036308410108	23.47734786918709
33	28.963318527593167	22.311813286393733	26.59567622428375	22.129191961729347
34	26.48473499136988	23.787282811358363	27.113809251130387	22.61417294614137
35	26.03160277499701	24.922027536558883	27.11714889176876	21.92922079667535
36	25.10350849612724	26.702951997960376	26.041540242262418	22.151999263649966
37	26.573846378159725	26.405805435794594	25.211517361651314	21.808830824394366
38	25.74113549410798	27.203816639067117	25.115156511036695	21.93989135578821
39	26.98568108712634	24.47647060260964	25.090475752172598	23.44737255809142
40	25.247113043577425	26.327934790665626	24.791455733063337	23.633496432693615
41	24.128089065772183	26.010343111421	25.188628605080975	24.672939217725833
42	23.65434882302104	25.32319168641263	25.66269466643099	25.35976482413534
43	24.545544145569234	23.727006370568155	26.19426771048085	25.533181773381763
44	23.5603501572482	23.74427475630805	25.59248075837537	27.102894328068377
45	25.658296115346303	25.229193020639794	25.204919535024278	23.90759132898963
46	24.974158512377443	24.46213458425954	25.62327061596821	24.940436287394814
47	24.75789641738014	24.38279775543567	25.944446299800354	24.914859527383832
48	24.960392676575353	25.66684885356653	24.799112470136688	24.573645999721425
49	25.27285271288784	24.36512209644719	25.65943648044233	24.70258871022264
50	23.96387649194373	24.678559588556272	25.88009712652432	25.47746679297568
51	23.24153665825783	25.513795566749238	25.80597339528231	25.438694379710626
52	23.528419934559334	26.263422708090157	26.182782604870823	24.025374752479685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	75.0
10	149.0
11	689.5
12	1230.0
13	2669.5
14	5121.0
15	6133.0
16	5452.5
17	4772.0
18	4719.0
19	4666.0
20	5076.5
21	5487.0
22	5445.5
23	5404.0
24	5982.5
25	6561.0
26	7383.0
27	8205.0
28	8988.0
29	9771.0
30	12976.0
31	16181.0
32	17435.0
33	18689.0
34	20471.0
35	22253.0
36	25047.5
37	27842.0
38	28056.5
39	33479.5
40	38688.0
41	38718.5
42	38749.0
43	40335.0
44	41921.0
45	49003.0
46	56085.0
47	57391.5
48	58698.0
49	60872.5
50	63047.0
51	66452.0
52	69857.0
53	71338.0
54	72819.0
55	74258.0
56	75697.0
57	82782.5
58	89868.0
59	95535.0
60	101202.0
61	95017.0
62	88832.0
63	82740.5
64	69621.0
65	62593.0
66	49849.0
67	37105.0
68	30707.0
69	24309.0
70	21150.0
71	17991.0
72	18735.0
73	19479.0
74	16205.5
75	12932.0
76	9995.5
77	7059.0
78	4835.5
79	2612.0
80	1755.5
81	899.0
82	732.0
83	565.0
84	406.0
85	247.0
86	144.0
87	41.0
88	22.5
89	3.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1227677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.559630407180991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81454942946242	8.30149649262816
2	10.168282326156017	2.3508317113246835
3	3.8747396524656823	1.3437167491965663
4	2.0011276714931965	0.9252918512017619
5	1.2139770698530687	0.7016563125147008
6	0.8397683674033979	0.5824447172895034
7	0.5619506875423854	0.45471595785358565
8	0.3778246412596691	0.3494010569350017
9	0.3134414765411496	0.3260940860787103
>10	4.229037707814718	12.82817752460404
>50	2.451416166511083	20.416364544485642
>100	2.0675779959054257	42.25196524591792
>500	0.06813695336195105	5.343336177994313
>1k	0.018169854229853614	3.824507571975391
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4293	0.349684811232922	No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3950	0.3217458663801635	No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3932	0.3202796826852666	No Hit
AGTCGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2818	0.2295392029010888	No Hit
AGTCGTGGGACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTTTT	2691	0.21919446238709367	No Hit
AGTCGTGGGGACATGCCCAGATATTTCTCTAGATCTTAACATTTTCCCCAGC	2343	0.1908482442857527	No Hit
AGTCGTGGGCGGTAGATGCTTAATAATGTGTATTGAATGACTATCTCATGGA	1993	0.1623391168849787	No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1923	0.1566372914048239	No Hit
AGTCGTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	1642	0.13374853483448823	No Hit
AGTCGTGGGAGGCAAAATTTGTTCCTGAAGTTGCTTTTTACCCCAGCAGACA	1605	0.13073471279497784	No Hit
AGTCGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	1459	0.11884233393636925	No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1439	0.11721324094203932	No Hit
AGTCGTGGGGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGA	1300	0.1058910446314462	No Hit
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1229	0.10010776450157494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.072732485824855E-4	0.0	0.0	0.0	0.0
9	7.330918474484739E-4	0.0	0.0	0.0	0.0
10	0.005864734779587791	0.0	0.0	0.0	0.0
11	0.00953019401683016	0.0	0.0	0.0	0.0
12	0.012788380005490043	0.0	0.0	0.0	0.0
13	0.018327296186211844	0.0	0.0	0.0	0.0
14	0.028753491349923475	0.0	0.0	0.0	0.0
15	0.03698041097128968	0.0	0.0	0.0	0.0
16	0.05261970371685712	0.0	0.0	0.0	0.0
17	0.059461894293042876	0.0	0.0	0.0	0.0
18	0.06565244767149665	0.0	0.0	0.0	0.0
19	0.07534555098775982	0.0	0.0	0.0	0.0
20	0.08023282997074964	0.0	0.0	0.0	0.0
21	0.08756374844523437	0.0	0.0	0.0	0.0
22	0.09758267036036351	0.0	0.0	0.0	0.0
23	0.1036103144393843	0.0	0.0	0.0	0.0
24	0.11403650960309593	0.0	0.0	0.0	0.0
25	0.11998269903240022	0.0	0.0	0.0	0.0
26	0.12429979546737456	0.0	0.0	0.0	0.0
27	0.12983871164809638	0.0	0.0	0.0	0.0
28	0.1370067208231481	0.0	0.0	0.0	0.0
29	0.1427900009530194	0.0	0.0	0.0	0.0
30	0.14669982413941127	0.0	0.0	0.0	0.0
31	0.15191292172126708	0.0	0.0	0.0	0.0
32	0.15614856350652492	0.0	0.0	0.0	0.0
33	0.1622576622352622	0.0	0.0	0.0	0.0
34	0.16991439930861293	0.0	0.0	0.0	0.0
35	0.1794445933254431	0.0	0.0	0.0	0.0
36	0.19369915702583007	0.0	0.0	0.0	0.0
37	0.2016002580483303	0.0	0.0	0.0	0.0
38	0.2072206288787686	0.0	0.0	0.0	0.0
39	0.21316681830807288	0.0	0.0	0.0	0.0
40	0.21984609958482565	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAC	35	1.0196527E-7	46.000004	20
GGTCGTA	35	1.0196527E-7	46.000004	9
CATGACG	35	1.0196527E-7	46.000004	33
GACGTAT	35	1.0196527E-7	46.000004	9
AACGGTA	35	1.0196527E-7	46.000004	36
GGACGTA	55	1.8189894E-12	46.000004	8
TCGCGTT	35	1.0196527E-7	46.000004	21
GATACGT	20	6.311624E-4	46.0	34
CTCACGA	30	1.8615083E-6	46.0	44
CGAACCG	45	3.092282E-10	46.0	13
CGTATTA	30	1.8615083E-6	46.0	11
CGTATCG	30	1.8615083E-6	46.0	37
TTTACGC	20	6.311624E-4	46.0	13
CGACGAA	20	6.311624E-4	46.0	37
TTATGCG	25	3.417293E-5	46.0	23
CCGGATT	40	5.6115823E-9	46.0	21
CCTACGC	20	6.311624E-4	46.0	38
CACGACC	20	6.311624E-4	46.0	32
TAACGGT	20	6.311624E-4	46.0	40
TAACGAA	20	6.311624E-4	46.0	31
>>END_MODULE