FastQCFastQC Report
Fri 17 Jun 2016
SRR1527067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193260
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC40710.3411662169183581No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT40500.33940633223270705No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37400.3134270821111912No Hit
AGTCGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG26630.22317013894708615No Hit
AGTCGTGGGGACATGCCCAGATATTTCTCTAGATCTTAACATTTTCCCCAGC22870.19165982267066692No Hit
AGTCGTGGGACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTTTT22620.18956472185441564No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT19630.16450731609205035No Hit
AGTCGTGGGCGGTAGATGCTTAATAATGTGTATTGAATGACTATCTCATGGA17610.14757890149674002No Hit
AGTCGTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC15270.12796875785662806No Hit
AGTCGTGGGAGGCAAAATTTGTTCCTGAAGTTGCTTTTTACCCCAGCAGACA14640.12268910379967485No Hit
AGTCGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA14440.12101302314667381No Hit
AGTCGTGGGGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGA13380.1121297956857684No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT13210.11070512713071752No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG12610.10567688517171446No Hit
AGTCGTGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA12220.10240852789836247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA301.8614628E-646.012
CGTATTT253.4172357E-546.039
GCGACGG1150.046.043
TAGACGC253.4172357E-546.015
TAGACCT253.4172357E-546.025
CGCATTA750.046.045
TAGAACG253.4172357E-546.035
CGTCTAA301.8614628E-646.020
AAGCGAC206.3115516E-446.029
CGACAAT405.6115823E-946.011
TCGATAT206.3115516E-446.012
CCGTCTA253.4172357E-546.018
CCGTATA206.3115516E-446.031
TCAATCG206.3115516E-446.030
TACGATA206.3115516E-446.028
TGCGATA253.4172357E-546.014
CTACGGG253.4172357E-546.012
TGCGACG1150.046.042
CGCTAAA453.092282E-1046.029
CGTAACG301.8614628E-646.028
Produced by FastQC (version 0.11.5)