##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1193260
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68374956002883	31.0	31.0	33.0	30.0	34.0
2	32.42886629904631	34.0	31.0	34.0	31.0	34.0
3	30.991100011732566	31.0	31.0	33.0	30.0	33.0
4	35.85322896937801	37.0	35.0	37.0	35.0	37.0
5	36.1071912240417	37.0	35.0	37.0	35.0	37.0
6	35.15486901429697	35.0	35.0	37.0	33.0	37.0
7	36.25849605283006	37.0	35.0	37.0	35.0	37.0
8	36.62721871176441	37.0	37.0	37.0	35.0	37.0
9	38.6554455860416	39.0	39.0	39.0	39.0	39.0
10	38.058097983674976	39.0	38.0	39.0	37.0	39.0
11	37.69140505841141	39.0	37.0	39.0	35.0	39.0
12	37.3422079010442	39.0	37.0	39.0	35.0	39.0
13	37.227744163049124	39.0	37.0	39.0	34.0	39.0
14	38.14152322209745	40.0	38.0	40.0	34.0	40.0
15	38.17728324086955	40.0	38.0	40.0	35.0	40.0
16	38.20179759650034	40.0	38.0	40.0	35.0	40.0
17	38.18083820793456	40.0	38.0	40.0	34.0	40.0
18	38.14073797831151	40.0	38.0	40.0	34.0	40.0
19	38.08245311164373	40.0	38.0	40.0	34.0	40.0
20	38.05340076764494	40.0	38.0	40.0	34.0	40.0
21	33.79509243584801	39.0	34.0	40.0	10.0	40.0
22	34.46494728726346	39.0	34.0	40.0	17.0	40.0
23	36.54410438630307	39.0	34.0	40.0	30.0	40.0
24	37.31778321572834	39.0	36.0	40.0	33.0	40.0
25	37.601945929638134	39.0	37.0	40.0	33.0	40.0
26	37.67918810653168	39.0	37.0	40.0	34.0	40.0
27	37.667995239930946	40.0	37.0	40.0	34.0	40.0
28	37.556110152020516	40.0	37.0	40.0	33.0	40.0
29	37.53745034611065	40.0	37.0	40.0	33.0	40.0
30	37.48253356351508	40.0	36.0	40.0	33.0	40.0
31	37.30424299817307	39.0	36.0	40.0	33.0	40.0
32	37.273094715317704	39.0	36.0	40.0	33.0	40.0
33	36.99097933392555	39.0	35.0	40.0	32.0	40.0
34	36.82956941488025	39.0	35.0	40.0	31.0	40.0
35	36.768121784020245	39.0	35.0	40.0	31.0	40.0
36	36.64653470324992	39.0	35.0	40.0	31.0	40.0
37	36.67598008816184	39.0	35.0	40.0	31.0	40.0
38	36.568131840504165	38.0	35.0	40.0	31.0	40.0
39	36.41392990630709	38.0	35.0	40.0	31.0	40.0
40	31.590278732212592	37.0	31.0	40.0	9.0	40.0
41	32.4518973232992	37.0	31.0	40.0	14.0	40.0
42	34.26169569079664	37.0	31.0	40.0	24.0	40.0
43	35.12753297688685	37.0	33.0	40.0	28.0	40.0
44	35.91477716507718	38.0	35.0	40.0	30.0	40.0
45	36.18346630239847	38.0	35.0	40.0	31.0	40.0
46	36.23281933526641	38.0	35.0	40.0	31.0	40.0
47	36.19449910329685	38.0	35.0	40.0	31.0	40.0
48	36.075639005748954	38.0	35.0	40.0	31.0	40.0
49	35.940700266496826	38.0	35.0	40.0	31.0	40.0
50	35.82423780232305	38.0	35.0	40.0	31.0	40.0
51	35.65244204951142	37.0	35.0	40.0	30.0	40.0
52	35.132948393476696	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	4.0
15	8.0
16	31.0
17	60.0
18	102.0
19	236.0
20	394.0
21	687.0
22	1189.0
23	1857.0
24	2941.0
25	4457.0
26	6216.0
27	8708.0
28	11876.0
29	16540.0
30	22158.0
31	30973.0
32	40413.0
33	54517.0
34	94120.0
35	119776.0
36	154080.0
37	166860.0
38	365838.0
39	89215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.35764208973737	0.5914888624440607	0.03234835660291973	0.018520691215661297
8	99.0928213465632	0.6275245964835828	0.23398085915894273	0.045673197794277864
9	97.99230678980273	1.2343495969026028	0.4323450044416137	0.34099860885305805
10	48.42347853778724	38.39238724167407	4.663862025040645	8.520272195498048
11	30.861002631446627	24.19305096961266	25.36538558235422	19.580560816586495
12	28.37814055612356	16.989256323014263	29.50362871461375	25.128974406248428
13	24.560615456815782	17.160551765750967	31.662672007777015	26.61616076965624
14	26.319159277944454	19.95072322880177	30.739738196201998	22.990379297051774
15	24.519132460654006	21.73482727988871	29.808926805557885	23.937113453899403
16	29.208303303554967	21.78527730754404	26.046544759733838	22.959874629167157
17	28.090525116068587	21.141997552922245	25.145148584549887	25.622328746459278
18	26.41159512595746	21.026934616093726	27.381459195816504	25.180011062132312
19	26.88877528786685	21.5571627306706	26.822653906105963	24.731408075356587
20	25.80032851180799	22.625999363089353	26.70147327489399	24.872198850208672
21	31.962941856762146	20.662470878098656	26.635016677002497	20.7395705881367
22	25.862930124197575	21.76977356150378	28.626451904865664	23.74084440943298
23	26.08299951393661	22.898278665169368	28.203241539982905	22.815480280911117
24	25.866868913732127	22.571778153964768	26.25840135427317	25.302951578029937
25	26.160099223974658	24.01689489298225	25.27982166501852	24.54318421802457
26	26.39114694199085	23.579270234483683	26.306085848851048	23.723496974674422
27	26.053584298476444	20.206995960645628	26.793909122906996	26.945510617970935
28	26.050651157333693	22.924174111258232	25.61847376095738	25.406700970450697
29	25.940029834235624	23.91549201347569	25.75725323902586	24.387224913262827
30	26.2313326517272	22.987194743811074	25.95704205286358	24.82443055159814
31	27.69086368436049	23.21698540133751	25.17942443390376	23.912726480398234
32	27.846403968958988	23.180111626971488	25.545229036421237	23.42825536764829
33	28.978512646028527	22.349781271474782	26.506042270754072	22.16566381174262
34	26.436987747850427	23.793976166133117	27.066104620954363	22.702931465062097
35	26.046125739570584	24.85024219365436	27.085211940398572	22.01842012637648
36	25.19266547106247	26.578700367061664	26.09883847610747	22.1297956857684
37	26.44645760353988	26.395672359753952	25.233478034963042	21.924392001743122
38	25.717111107386486	27.165999027873223	25.12805256188928	21.988837302851014
39	26.68345540787423	24.483096726614484	25.168948929822506	23.664498935688787
40	26.106045622915374	30.159143858002444	22.631362821178953	21.103447697903224
41	24.108073680505505	25.988552369140006	25.191576018638017	24.711797931716475
42	23.5190151350083	25.414410941454502	25.756499002732014	25.310074920805185
43	24.552905485811976	23.683773863198297	26.206442854030136	25.556877796959586
44	23.577677957863333	23.73690561989843	25.612607478671872	27.07280894356636
45	25.64059802557699	25.266999648023063	25.182022358915912	23.910379967484037
46	24.949885188475267	24.50899217270335	25.654928515160147	24.88619412366123
47	24.467509176541576	24.37842548983457	26.08207766957746	25.07198766404639
48	24.84848230896871	25.60397566330892	24.909240232639995	24.63830179508238
49	25.232137170440645	24.402225835107185	25.63917335702194	24.726463637430232
50	23.91892797881434	24.714144444630676	25.955282168177934	25.411645408377055
51	23.38978931666192	25.510869383034713	25.7385649397449	25.360776360558468
52	23.443591505623253	26.305080200459248	26.235858069490302	24.015470224427197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	1.0
8	0.0
9	52.5
10	105.0
11	602.5
12	1100.0
13	2476.0
14	4792.5
15	5733.0
16	5294.5
17	4856.0
18	4712.5
19	4569.0
20	4898.5
21	5228.0
22	5161.0
23	5094.0
24	5722.5
25	6351.0
26	7139.5
27	7928.0
28	8686.0
29	9444.0
30	12243.0
31	15042.0
32	16406.5
33	17771.0
34	19447.5
35	21124.0
36	24020.0
37	26916.0
38	26880.5
39	31936.5
40	37028.0
41	37149.5
42	37271.0
43	38871.5
44	40472.0
45	47467.5
46	54463.0
47	55902.0
48	57341.0
49	58846.5
50	60352.0
51	63697.5
52	67043.0
53	69666.5
54	72290.0
55	73046.0
56	73802.0
57	80112.0
58	86422.0
59	92342.5
60	98263.0
61	92528.5
62	86794.0
63	81329.5
64	68501.5
65	61138.0
66	49437.0
67	37736.0
68	31178.5
69	24621.0
70	20920.0
71	17219.0
72	18105.0
73	18991.0
74	15795.0
75	12599.0
76	9901.5
77	7204.0
78	4941.0
79	2678.0
80	1790.5
81	903.0
82	713.0
83	523.0
84	381.0
85	239.0
86	135.5
87	32.0
88	18.0
89	2.0
90	0.0
91	2.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1193260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.542681259220121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.25097048205907	7.877991979068491
2	10.04852574765786	2.319738596605625
3	4.804534281277963	1.6637162342336327
4	2.760736287500583	1.2746519602952764
5	1.6819613187179145	0.9707171696149217
6	1.190652816360311	0.8245995569783889
7	0.8587647902294835	0.6938713775181969
8	0.5975993800546832	0.5518319331743003
9	0.4916094358281277	0.5107041919610197
>10	4.779639756207084	13.76898708728824
>50	2.4665569591483334	20.494213996822058
>100	1.9765176896270757	39.81938551027998
>500	0.07557044378944673	5.7452143213206135
>1k	0.01636061154204517	3.484376084839247
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4071	0.3411662169183581	No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4050	0.33940633223270705	No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3740	0.3134270821111912	No Hit
AGTCGTGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2663	0.22317013894708615	No Hit
AGTCGTGGGGACATGCCCAGATATTTCTCTAGATCTTAACATTTTCCCCAGC	2287	0.19165982267066692	No Hit
AGTCGTGGGACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTTTT	2262	0.18956472185441564	No Hit
AGTCGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1963	0.16450731609205035	No Hit
AGTCGTGGGCGGTAGATGCTTAATAATGTGTATTGAATGACTATCTCATGGA	1761	0.14757890149674002	No Hit
AGTCGTGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	1527	0.12796875785662806	No Hit
AGTCGTGGGAGGCAAAATTTGTTCCTGAAGTTGCTTTTTACCCCAGCAGACA	1464	0.12268910379967485	No Hit
AGTCGTGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	1444	0.12101302314667381	No Hit
AGTCGTGGGGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGA	1338	0.1121297956857684	No Hit
AGTCGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1321	0.11070512713071752	No Hit
AGTCGTGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	1261	0.10567688517171446	No Hit
AGTCGTGGGATATTATTTGATCTTATCTTGGATCCTTTTGAAAAGTTAAGAA	1222	0.10240852789836247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	8.380403265005112E-5	0.0	0.0	0.0	0.0
9	3.352161306002045E-4	0.0	0.0	0.0	0.0
10	0.004776829861052914	0.0	0.0	0.0	0.0
11	0.009218443591505624	0.0	0.0	0.0	0.0
12	0.012989625060757924	0.0	0.0	0.0	0.0
13	0.01810167105241104	0.0	0.0	0.0	0.0
14	0.028828587231617585	0.0	0.0	0.0	0.0
15	0.0372089904966227	0.0	0.0	0.0	0.0
16	0.04894155506762986	0.0	0.0	0.0	0.0
17	0.05447262122253323	0.0	0.0	0.0	0.0
18	0.06167976803043763	0.0	0.0	0.0	0.0
19	0.07106581968724335	0.0	0.0	0.0	0.0
20	0.07751873020129729	0.0	0.0	0.0	0.0
21	0.08548011330305215	0.0	0.0	0.0	0.0
22	0.09511757705780802	0.0	0.0	0.0	0.0
23	0.10207331176776227	0.0	0.0	0.0	0.0
24	0.11204599165311835	0.0	0.0	0.0	0.0
25	0.11699042957947137	0.0	0.0	0.0	0.0
26	0.12235388766907464	0.0	0.0	0.0	0.0
27	0.12763354172602787	0.0	0.0	0.0	0.0
28	0.1355949248277827	0.0	0.0	0.0	0.0
29	0.14087457888473592	0.0	0.0	0.0	0.0
30	0.14464576035398824	0.0	0.0	0.0	0.0
31	0.1500092184435915	0.0	0.0	0.0	0.0
32	0.1567135410555956	0.0	0.0	0.0	0.0
33	0.16107135075339826	0.0	0.0	0.0	0.0
34	0.16660241690830163	0.0	0.0	0.0	0.0
35	0.17422858387945628	0.0	0.0	0.0	0.0
36	0.18579354038516332	0.0	0.0	0.0	0.0
37	0.19157601863801685	0.0	0.0	0.0	0.0
38	0.19811273318472084	0.0	0.0	0.0	0.0
39	0.20406281950287447	0.0	0.0	0.0	0.0
40	0.21219181066992943	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTA	30	1.8614628E-6	46.0	12
CGTATTT	25	3.4172357E-5	46.0	39
GCGACGG	115	0.0	46.0	43
TAGACGC	25	3.4172357E-5	46.0	15
TAGACCT	25	3.4172357E-5	46.0	25
CGCATTA	75	0.0	46.0	45
TAGAACG	25	3.4172357E-5	46.0	35
CGTCTAA	30	1.8614628E-6	46.0	20
AAGCGAC	20	6.3115516E-4	46.0	29
CGACAAT	40	5.6115823E-9	46.0	11
TCGATAT	20	6.3115516E-4	46.0	12
CCGTCTA	25	3.4172357E-5	46.0	18
CCGTATA	20	6.3115516E-4	46.0	31
TCAATCG	20	6.3115516E-4	46.0	30
TACGATA	20	6.3115516E-4	46.0	28
TGCGATA	25	3.4172357E-5	46.0	14
CTACGGG	25	3.4172357E-5	46.0	12
TGCGACG	115	0.0	46.0	42
CGCTAAA	45	3.092282E-10	46.0	29
CGTAACG	30	1.8614628E-6	46.0	28
>>END_MODULE