FastQCFastQC Report
Fri 17 Jun 2016
SRR1527066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521218
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT49210.3234907817288515No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC45260.2975247466175131No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC37500.24651299156333936No Hit
AGGGTCGGGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCCCAGCAT35440.23297121122679326No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC33990.2234393755530108No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG33890.22278200757550856No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC33850.2225190603845077No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28100.18472040167812898No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT24500.16105515448804839No Hit
AGGGTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT23600.15513884269052824No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG23370.1536268963422731No Hit
AGGGTCGGGACATAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTT23010.15126037162326503No Hit
AGGGTCGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAA22180.14580421740999647No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC20450.13443175139920774No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG19120.125688757298428No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA19060.12529433651192662No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC18690.12286207499516834No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG17930.11786607836615133No Hit
AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC15590.10248366769259895No Hit
AGGGTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC15540.10215498370384783No Hit
AGGGTCGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT15450.10156335252409583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA302.5282588E-644.00000411
CCTTACG207.857286E-444.029
CTCGTAA207.857286E-444.034
CAACGTC408.3164196E-944.014
CGACGTA207.857286E-444.016
ACGTTAT207.857286E-444.016
TAGACGT207.857286E-444.016
GACGTTA207.857286E-444.015
TAGCGAT454.802132E-1044.026
AAGCGTA254.443521E-544.015
TAACGCG207.857286E-444.034
GCGCATA408.3164196E-944.09
ATCACGA254.443521E-544.031
GTCCGAT408.3164196E-944.044
AATTCGG207.857286E-444.022
AATTCGC1650.044.022
CATACGC207.857286E-444.015
CGTAACT254.443521E-544.019
CGTAAAC254.443521E-544.012
CCGGTTA207.857286E-444.024
Produced by FastQC (version 0.11.5)