##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1521218
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43527094735929	31.0	31.0	33.0	30.0	33.0
2	32.25193036106594	33.0	31.0	34.0	31.0	34.0
3	32.919625589494736	34.0	31.0	34.0	31.0	34.0
4	36.440203179294485	37.0	37.0	37.0	35.0	37.0
5	33.30416942213411	35.0	33.0	35.0	30.0	35.0
6	34.981103957486695	35.0	35.0	36.0	33.0	37.0
7	35.999480679297776	37.0	35.0	37.0	35.0	37.0
8	36.534536798802016	37.0	37.0	37.0	35.0	37.0
9	38.66684525163389	39.0	39.0	39.0	38.0	39.0
10	37.99097828187676	39.0	38.0	39.0	35.0	39.0
11	37.450707919574974	39.0	37.0	39.0	35.0	39.0
12	36.93763878681425	39.0	37.0	39.0	33.0	39.0
13	36.79529692654176	39.0	37.0	39.0	33.0	39.0
14	37.619636370329566	39.0	37.0	40.0	33.0	40.0
15	37.579532322126084	39.0	37.0	40.0	33.0	40.0
16	37.43837832578894	39.0	37.0	40.0	33.0	40.0
17	37.432200381536376	39.0	37.0	40.0	33.0	40.0
18	37.33087565358811	39.0	36.0	40.0	33.0	40.0
19	37.26452684625083	39.0	36.0	40.0	33.0	40.0
20	36.956496701984854	39.0	36.0	40.0	31.0	40.0
21	37.06640402624739	39.0	36.0	40.0	32.0	40.0
22	36.81941970184418	39.0	36.0	40.0	31.0	40.0
23	36.805580791181804	38.0	35.0	40.0	31.0	40.0
24	36.80225450921564	38.0	35.0	40.0	31.0	40.0
25	36.71920066683408	38.0	35.0	40.0	31.0	40.0
26	36.48387213403996	38.0	35.0	40.0	31.0	40.0
27	36.30292502455269	38.0	35.0	40.0	30.0	40.0
28	36.08535594503878	38.0	35.0	40.0	30.0	40.0
29	36.57773244860368	39.0	35.0	40.0	31.0	40.0
30	36.736655758740696	39.0	35.0	40.0	31.0	40.0
31	36.77441760484033	39.0	35.0	40.0	31.0	40.0
32	36.02442779404398	39.0	35.0	40.0	30.0	40.0
33	36.158603171931965	38.0	35.0	40.0	30.0	40.0
34	36.121425068596345	38.0	35.0	40.0	30.0	40.0
35	36.03598826729634	38.0	35.0	40.0	30.0	40.0
36	35.816583159021256	38.0	35.0	40.0	29.0	40.0
37	35.73193125508639	38.0	34.0	40.0	29.0	40.0
38	35.48798725757912	38.0	34.0	40.0	29.0	40.0
39	35.446617776018954	37.0	34.0	40.0	29.0	40.0
40	35.25981088838023	37.0	34.0	40.0	28.0	40.0
41	35.12568349835461	37.0	34.0	40.0	28.0	40.0
42	35.0066387592048	37.0	34.0	40.0	28.0	40.0
43	34.82566864183832	36.0	33.0	40.0	28.0	40.0
44	34.676524337734634	36.0	33.0	40.0	27.0	40.0
45	34.54540835041394	36.0	33.0	40.0	27.0	40.0
46	34.21957010763743	35.0	33.0	39.0	26.0	40.0
47	34.095125090552436	35.0	33.0	39.0	26.0	40.0
48	33.934948837050314	35.0	33.0	39.0	26.0	40.0
49	33.58174436537038	35.0	33.0	39.0	24.0	40.0
50	33.11726129982685	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	6.0
14	12.0
15	32.0
16	86.0
17	166.0
18	311.0
19	566.0
20	1079.0
21	1748.0
22	2516.0
23	4045.0
24	5699.0
25	7750.0
26	10837.0
27	14794.0
28	19579.0
29	26008.0
30	33291.0
31	43425.0
32	57667.0
33	81495.0
34	124629.0
35	150794.0
36	223043.0
37	296750.0
38	379799.0
39	35089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.31561419862241	0.5473245780683637	0.1158939744336446	0.021167248875572076
8	99.47252793485221	0.31560236599882463	0.0918343064570627	0.12003539269190872
9	96.46973675041973	1.7029774825172987	0.7609034339588409	1.066382333104131
10	52.90273977825664	34.35845486971624	4.688611362736965	8.050193989290161
11	32.52249184535024	22.197475969913583	25.243521967265703	20.036510217470475
12	26.89561916832433	17.83537928160198	28.61049501123442	26.658506538839273
13	23.132384707517264	17.974807029630206	31.999226935258456	26.893581327594074
14	25.83357546387171	17.372395015047154	31.2910444130953	25.502985107985836
15	25.3148463928247	18.678650923141852	28.779241371059243	27.22726131297421
16	29.787906795738678	19.093384380148013	25.354288471474835	25.764420352638478
17	30.116590784489798	19.590025887150954	24.068936865064707	26.224446463294544
18	29.173530683965087	19.28073425373615	24.187328837812856	27.358406224485904
19	28.95140604436708	19.147222817505448	24.56689310802265	27.334478030104826
20	29.353320825811945	19.416217793899364	24.95178205885021	26.27867932143848
21	31.00048776703931	18.753064978195106	25.534933191692446	24.711514063073142
22	30.848701501034036	18.370936972873054	24.71979689958967	26.060564626503236
23	28.237635894395147	20.974114163781916	25.053739832160808	25.73451010966213
24	28.034706399740205	19.88656458180221	24.685087870377554	27.39364114808003
25	28.225277376418106	20.321742182908693	24.31512117263929	27.137859268033903
26	27.9302506281151	21.24442387613084	24.283238825730436	26.54208667002362
27	28.341762982031504	18.721445578477248	23.41860272492174	29.51818871456951
28	28.88803577133586	20.098434280951185	24.363634929378957	26.649895018333993
29	29.83668349966934	19.928964816351108	23.144743225494306	27.08960845848524
30	28.21469375198032	19.438305357943435	24.823661040035024	27.523339850041218
31	29.15657059014553	20.897662267998406	22.65191445276088	27.29385268909519
32	30.350087890098592	21.294449579218757	23.861142847376247	24.494319683306404
33	30.3519285204356	20.163053553139658	23.26254356706271	26.222474359362035
34	29.619094699116104	22.55501841287705	24.206458245958174	23.619428642048675
35	28.275566026697028	23.548104216489683	24.562620216168884	23.613709540644404
36	29.78179327354791	22.688135428321253	22.717716987308854	24.812354310821984
37	30.580429629415377	23.0689486976883	21.555095982298393	24.795525690597927
38	30.583124838123137	23.33656320132946	22.756501697981484	23.323810262565917
39	29.47440800726786	22.909405489548508	22.19648991794733	25.419696585236306
40	27.865828566319884	23.7004821136747	23.524767653288354	24.908921666717067
41	26.766709307936143	22.79561509264287	23.615352960588158	26.82232263883283
42	27.44156327363994	20.576735221381814	24.01667611085328	27.96502539412497
43	26.95603128545678	20.28788773206733	25.654311216406857	27.101769766069033
44	26.973122852871846	20.471030450599454	26.02092533745985	26.534921359068854
45	27.51190164723268	20.862361607606537	25.463871713324455	26.161865031836328
46	27.781422518008597	21.444921109269018	24.02916610242582	26.744490270296566
47	26.912250578155135	21.856301989589923	24.71111964228664	26.520327789968302
48	28.600897438762885	20.058203360728047	25.778816711345776	25.56208248916329
49	26.695121935186144	22.504926972991377	24.888083101830244	25.91186798999223
50	24.85712107008989	23.011166052465853	25.87190001696009	26.259812860484168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	3.0
12	6.0
13	114.5
14	223.0
15	817.0
16	1411.0
17	1914.5
18	2418.0
19	2513.5
20	2609.0
21	3170.5
22	3732.0
23	3889.0
24	4046.0
25	3956.5
26	3867.0
27	4409.0
28	4951.0
29	7337.5
30	9724.0
31	9838.5
32	9953.0
33	10990.0
34	12027.0
35	12285.0
36	12543.0
37	17660.0
38	22777.0
39	23833.5
40	24890.0
41	27388.0
42	29886.0
43	32863.5
44	35841.0
45	40561.5
46	45282.0
47	45380.5
48	45479.0
49	50036.5
50	54594.0
51	60917.0
52	67240.0
53	71767.0
54	76294.0
55	77671.0
56	79048.0
57	90699.0
58	102350.0
59	117725.0
60	133100.0
61	134818.0
62	136536.0
63	135514.5
64	134493.0
65	130560.5
66	126628.0
67	119266.5
68	111905.0
69	95441.0
70	78977.0
71	66290.0
72	53603.0
73	44454.5
74	35306.0
75	29736.5
76	24167.0
77	23275.5
78	22384.0
79	15513.5
80	8643.0
81	5611.0
82	2579.0
83	1844.5
84	1110.0
85	749.0
86	388.0
87	257.0
88	126.0
89	93.0
90	60.0
91	37.0
92	14.0
93	8.0
94	2.0
95	3.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1521218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.344643012135055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.68429063229067	5.648012428560626
2	10.661966089038053	1.779406016410246
3	4.512141424936514	1.1295662823408479
4	2.467979498493563	0.8237763150478756
5	1.4043864746063848	0.5859551890830577
6	0.8690175979367828	0.435098497562734
7	0.614054966055563	0.35868486370936675
8	0.4017253472482789	0.26818036893703034
9	0.3045554764972226	0.22872660558840094
>10	4.475227667142445	10.20836171548526
>50	3.4186784219847026	20.671338956972978
>100	3.0153282570789366	44.38533210768656
>500	0.12900673290508313	7.296524179006086
>1k	0.041641413785817975	6.1810364736089225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4921	0.3234907817288515	No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4526	0.2975247466175131	No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3750	0.24651299156333936	No Hit
AGGGTCGGGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCCCAGCAT	3544	0.23297121122679326	No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3399	0.2234393755530108	No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3389	0.22278200757550856	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	3385	0.2225190603845077	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2810	0.18472040167812898	No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	2450	0.16105515448804839	No Hit
AGGGTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	2360	0.15513884269052824	No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2337	0.1536268963422731	No Hit
AGGGTCGGGACATAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTT	2301	0.15126037162326503	No Hit
AGGGTCGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAA	2218	0.14580421740999647	No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	2045	0.13443175139920774	No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1912	0.125688757298428	No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1906	0.12529433651192662	No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1869	0.12286207499516834	No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1793	0.11786607836615133	No Hit
AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1559	0.10248366769259895	No Hit
AGGGTCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1554	0.10215498370384783	No Hit
AGGGTCGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT	1545	0.10156335252409583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.6294719100089536E-4	0.0	0.0	0.0	0.0
8	9.203151685031336E-4	0.0	0.0	0.0	0.0
9	0.0010517887640035814	0.0	0.0	0.0	0.0
10	0.005521891011018802	0.0	0.0	0.0	0.0
11	0.008151362921027755	0.0	0.0	0.0	0.0
12	0.01097804522428738	0.0	0.0	0.0	0.0
13	0.014199148314048348	0.0	0.0	0.0	0.0
14	0.019983986516068045	0.0	0.0	0.0	0.0
15	0.02760945505509401	0.0	0.0	0.0	0.0
16	0.034183134830116396	0.0	0.0	0.0	0.0
17	0.03839028988613072	0.0	0.0	0.0	0.0
18	0.04292612893089617	0.0	0.0	0.0	0.0
19	0.048645230335165636	0.0	0.0	0.0	0.0
20	0.051734859829426154	0.0	0.0	0.0	0.0
21	0.05594201488544048	0.0	0.0	0.0	0.0
22	0.06028064353695525	0.0	0.0	0.0	0.0
23	0.0648164825817207	0.0	0.0	0.0	0.0
24	0.06915511123323548	0.0	0.0	0.0	0.0
25	0.07237621432299644	0.0	0.0	0.0	0.0
26	0.07566305421050763	0.0	0.0	0.0	0.0
27	0.08039610364852375	0.0	0.0	0.0	0.0
28	0.08512915308653987	0.0	0.0	0.0	0.0
29	0.08854746656955151	0.0	0.0	0.0	0.0
30	0.09354346319856852	0.0	0.0	0.0	0.0
31	0.10084024774884336	0.0	0.0	0.0	0.0
32	0.10669082274861329	0.0	0.0	0.0	0.0
33	0.11089797780462761	0.0	0.0	0.0	0.0
34	0.11595971123139484	0.0	0.0	0.0	0.0
35	0.12667480926468133	0.0	0.0	0.0	0.0
36	0.13561501375871177	0.0	0.0	0.0	0.0
37	0.1414655887584817	0.0	0.0	0.0	0.0
38	0.14685600617400005	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	30	2.5282588E-6	44.000004	11
CCTTACG	20	7.857286E-4	44.0	29
CTCGTAA	20	7.857286E-4	44.0	34
CAACGTC	40	8.3164196E-9	44.0	14
CGACGTA	20	7.857286E-4	44.0	16
ACGTTAT	20	7.857286E-4	44.0	16
TAGACGT	20	7.857286E-4	44.0	16
GACGTTA	20	7.857286E-4	44.0	15
TAGCGAT	45	4.802132E-10	44.0	26
AAGCGTA	25	4.443521E-5	44.0	15
TAACGCG	20	7.857286E-4	44.0	34
GCGCATA	40	8.3164196E-9	44.0	9
ATCACGA	25	4.443521E-5	44.0	31
GTCCGAT	40	8.3164196E-9	44.0	44
AATTCGG	20	7.857286E-4	44.0	22
AATTCGC	165	0.0	44.0	22
CATACGC	20	7.857286E-4	44.0	15
CGTAACT	25	4.443521E-5	44.0	19
CGTAAAC	25	4.443521E-5	44.0	12
CCGGTTA	20	7.857286E-4	44.0	24
>>END_MODULE