FastQCFastQC Report
Fri 17 Jun 2016
SRR1527065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649189
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG52030.3154884006623862No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT42340.2567322483960298No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC36370.2205326375570053No Hit
AGGGTCGGGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCCCAGCATAT34590.2097394537557551No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC34080.20664702468910476No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG32750.1985824547701931No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG32340.19609638434406246No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC25830.1566224368462317No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT25150.15249919809069792No Hit
AGGGTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC23770.14413144885152643No Hit
AGGGTCGGGACATAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGT22830.1384316776306415No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC22240.1348541616515754No Hit
AGGGTCGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAATC21010.12739595037318344No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT18980.11508686997063405No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA18750.1136922450974388No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG18520.11229762022424356No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG17810.10799247387655386No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG17790.10787120214844995No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16720.10138316469488942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA351.01992555E-746.0000049
CTAGCGT351.01992555E-746.00000422
TAATACG206.312258E-446.013
CGTATAT600.046.046
GTCGACA405.6134013E-946.010
TAGCGTA453.110472E-1046.040
TAGCGAT253.4178072E-546.026
CCAATCG301.8618994E-646.034
CGACCAA253.4178072E-546.018
ATTCGTT253.4178072E-546.039
TTATCGA206.312258E-446.020
TACGATA301.8618994E-646.038
AACGCGT253.4178072E-546.035
TCGTATT501.6370905E-1146.043
ACGTCGT600.046.016
CATCGTA405.6134013E-946.038
TTACGAC206.312258E-446.015
CCGGTTA253.4178072E-546.024
ACGTAAA206.312258E-446.011
TGTACGG253.4178072E-546.039
Produced by FastQC (version 0.11.5)