##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1649189
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.652832392163663	31.0	31.0	33.0	31.0	34.0
2	32.34711788642782	33.0	31.0	34.0	31.0	34.0
3	32.92380557959094	34.0	31.0	34.0	31.0	34.0
4	36.41190851988462	37.0	37.0	37.0	35.0	37.0
5	33.60084926591191	35.0	33.0	35.0	32.0	35.0
6	35.237744733926796	35.0	35.0	37.0	35.0	37.0
7	36.17417530677199	37.0	35.0	37.0	35.0	37.0
8	36.5622599956706	37.0	37.0	37.0	35.0	37.0
9	38.66151726697183	39.0	39.0	39.0	38.0	39.0
10	38.026282008914684	39.0	38.0	39.0	35.0	39.0
11	37.64485756332355	39.0	37.0	39.0	35.0	39.0
12	37.23839353767215	39.0	37.0	39.0	34.0	39.0
13	37.17765641172722	39.0	37.0	39.0	34.0	39.0
14	38.059081160497676	40.0	38.0	40.0	34.0	40.0
15	37.939660645323244	40.0	38.0	40.0	34.0	40.0
16	32.91671057713822	39.0	34.0	40.0	3.0	40.0
17	34.478690435116896	39.0	34.0	40.0	16.0	40.0
18	36.39970797767872	39.0	34.0	40.0	30.0	40.0
19	37.32223717233137	39.0	36.0	40.0	33.0	40.0
20	37.57318900380733	39.0	37.0	40.0	33.0	40.0
21	37.757272210765414	40.0	37.0	40.0	33.0	40.0
22	37.757682715565046	40.0	37.0	40.0	33.0	40.0
23	37.68662718463439	40.0	37.0	40.0	33.0	40.0
24	37.63047837452226	40.0	37.0	40.0	33.0	40.0
25	37.56765355577802	40.0	37.0	40.0	33.0	40.0
26	37.42285632513921	39.0	36.0	40.0	33.0	40.0
27	37.27273405291935	39.0	36.0	40.0	33.0	40.0
28	37.136746000609996	39.0	36.0	40.0	32.0	40.0
29	36.86186301266865	39.0	35.0	40.0	32.0	40.0
30	36.816910008495086	39.0	35.0	40.0	31.0	40.0
31	36.77380094094734	39.0	35.0	40.0	32.0	40.0
32	36.63284014142709	38.0	35.0	40.0	31.0	40.0
33	36.239372806876595	38.0	35.0	40.0	31.0	40.0
34	36.127649408284924	38.0	35.0	40.0	30.0	40.0
35	35.96730635482046	38.0	35.0	40.0	30.0	40.0
36	35.84876505967478	38.0	34.0	40.0	30.0	40.0
37	35.51487428063127	37.0	34.0	40.0	29.0	40.0
38	35.42222995666355	37.0	34.0	40.0	29.0	40.0
39	35.02711999655588	37.0	34.0	40.0	28.0	40.0
40	34.925706513928965	36.0	33.0	40.0	28.0	40.0
41	34.85199088764235	36.0	33.0	39.0	28.0	40.0
42	34.785100434213426	36.0	33.0	39.0	28.0	40.0
43	34.58066419312765	36.0	33.0	39.0	28.0	40.0
44	35.025180861623504	36.0	34.0	39.0	29.0	40.0
45	35.15436071911709	36.0	34.0	40.0	29.0	40.0
46	35.098020906033206	35.0	34.0	39.0	30.0	40.0
47	34.79096089047405	35.0	34.0	39.0	29.0	40.0
48	34.70092208958464	35.0	34.0	39.0	29.0	40.0
49	34.554131151735795	35.0	33.0	39.0	28.0	40.0
50	34.11990135757636	35.0	33.0	39.0	27.0	40.0
51	34.17227134064076	35.0	33.0	38.0	27.0	40.0
52	33.78768049022884	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	2.0
14	5.0
15	15.0
16	38.0
17	94.0
18	201.0
19	383.0
20	722.0
21	1304.0
22	2179.0
23	3445.0
24	4944.0
25	7235.0
26	10119.0
27	13808.0
28	18615.0
29	24764.0
30	33104.0
31	44338.0
32	61559.0
33	89492.0
34	138281.0
35	187089.0
36	269957.0
37	327576.0
38	352165.0
39	57752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.33330867474862	0.5335349677932608	0.11514750583468603	0.018008851623434307
8	99.47501468903806	0.30736319487942254	0.10932646288569715	0.1082956531968137
9	96.55230540586919	1.6451722634579784	0.7433956932771199	1.059126637395714
10	53.26727258064419	33.938863283710965	4.7381470528847816	8.055717082760072
11	32.56503651188554	22.08558267124023	25.068442731548657	20.280938085325577
12	26.946214169509986	17.768369786604204	28.71387087835294	26.57154516553288
13	23.36342287027139	17.85398762664558	31.793445141824257	26.989144361258777
14	25.91698101309189	17.270913157921864	31.201639108677053	25.610466720309194
15	25.311835089853258	18.616604888827176	28.90523766530094	27.166322356018625
16	28.16196324375193	26.85398702028694	22.41974691803062	22.56430281793051
17	30.152274845393706	19.521110072890373	23.978573711078596	26.348041370637326
18	29.387535327970294	19.090352894665198	24.1005124336871	27.421599343677407
19	29.05064246729756	19.097386654895224	24.58244628117214	27.269524596635076
20	29.27705678366761	19.328651840389426	24.97075835456094	26.423533021382024
21	31.205155988792065	18.638433799885885	25.426194329455264	24.730215881866783
22	30.72625393450963	18.26200635585127	24.87556004799935	26.13617966163975
23	28.38704357111283	20.75292765110609	24.930253597374225	25.929775180406857
24	28.13388883869587	19.681431297443773	24.709235872904802	27.475443990955554
25	28.241214318067847	20.198291402622743	24.34548132445705	27.21501295485235
26	27.923664298027695	21.117046014738154	24.353000171599497	26.606289515634657
27	28.50097835966648	18.61248165007164	23.317945972232412	29.568594018029472
28	28.873464472537712	20.016808261515205	24.30594674109517	26.803780524851913
29	29.913975899669477	19.98461061770361	22.98693479037272	27.114478692254192
30	28.263225136718717	19.337989763453432	24.679948750567704	27.71883634926015
31	29.369223297026597	20.78839963157649	22.611901971211303	27.230475100185608
32	30.427197853005328	20.677557272089494	23.89604830010387	24.99919657480131
33	30.722070059890044	20.026267456307313	23.208316330026456	26.043346153776188
34	29.494982079070382	22.414532233722152	24.304915931406285	23.785569755801184
35	28.362789225492048	23.533991555849575	24.477546236362237	23.625672982296148
36	29.709390494358136	22.72280496656235	22.69776235470889	24.87004218437062
37	30.682292933071952	23.0447814046783	21.46697558618206	24.805950076067692
38	30.487651809465138	23.358390093555077	22.744633877621062	23.409324219358727
39	29.83060158659802	22.91544510665545	21.98328996858456	25.27066333816197
40	28.02898879388597	23.53732652837243	23.49639732013735	24.93728735760425
41	26.815543882477993	22.717772189846038	23.545148554835134	26.921535372840832
42	27.562092640685815	20.613465163786564	23.776535012057444	28.047907183470176
43	26.98029152510719	20.113279921221885	25.69935889700938	27.20706965666155
44	26.954885098069415	20.364130490804875	25.8325152544675	26.84846915665821
45	27.482538387049633	20.767237715022354	25.343608282616486	26.406615615311523
46	27.42808738113097	21.371777279620467	24.101179428191674	27.098955911056887
47	27.23053573604966	21.846252915827115	24.542244703305684	26.380966644817544
48	28.72878730090972	20.041729601640565	25.698873810096963	25.53060928735275
49	26.574819502191684	22.311815080018118	25.00750368817643	26.105861729613768
50	24.768780291403836	23.029864982121513	25.74526024609672	26.456094480377928
51	27.361691109994062	22.319152019568406	23.89101552338756	26.428141347049973
52	27.833862583366738	22.498755448890336	23.446736547478793	26.220645420264145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	103.0
14	826.5
15	1448.0
16	1935.0
17	2422.0
18	2537.0
19	2652.0
20	3240.5
21	3829.0
22	3837.5
23	3846.0
24	3958.0
25	4070.0
26	4080.0
27	4090.0
28	5827.0
29	7564.0
30	8833.5
31	10103.0
32	10041.5
33	9980.0
34	11532.5
35	13085.0
36	15524.5
37	17964.0
38	20736.0
39	24975.5
40	26443.0
41	30571.0
42	34699.0
43	37632.5
44	40566.0
45	41164.0
46	41762.0
47	46486.5
48	51211.0
49	53454.5
50	55698.0
51	61592.0
52	67486.0
53	71493.0
54	75500.0
55	80627.5
56	85755.0
57	95382.5
58	105010.0
59	118708.5
60	132407.0
61	144445.0
62	156483.0
63	147976.0
64	138026.5
65	136584.0
66	127520.5
67	118457.0
68	105296.0
69	92135.0
70	79623.0
71	67111.0
72	55370.5
73	43630.0
74	37159.0
75	30688.0
76	27540.0
77	24392.0
78	18699.5
79	13007.0
80	8395.0
81	3783.0
82	2666.0
83	1549.0
84	977.0
85	405.0
86	263.5
87	122.0
88	83.5
89	29.0
90	13.0
91	11.5
92	10.0
93	5.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1649189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.057509951721055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7417145388855	7.316005178800931
2	9.60628254230644	1.9323056453658252
3	3.700525244706775	1.1165420842570015
4	1.920382066834753	0.772570469931887
5	1.1136788534770994	0.5600418075933611
6	0.679083041747495	0.4097930670252261
7	0.4263885488103321	0.300188495107187
8	0.32761142756593825	0.26359641544335727
9	0.24013426123433618	0.21736374499021677
>10	3.8422827628572818	10.802495434220171
>50	2.86019930417139	21.013044718314568
>100	2.403276668550305	42.21498522605996
>500	0.10638795695058104	7.230099513323443
>1k	0.03137080781876108	5.494096452141817
>5k	6.819740830165451E-4	0.35687174742504935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5203	0.3154884006623862	No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4234	0.2567322483960298	No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3637	0.2205326375570053	No Hit
AGGGTCGGGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCCCAGCATAT	3459	0.2097394537557551	No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3408	0.20664702468910476	No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3275	0.1985824547701931	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3234	0.19609638434406246	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2583	0.1566224368462317	No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2515	0.15249919809069792	No Hit
AGGGTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	2377	0.14413144885152643	No Hit
AGGGTCGGGACATAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGT	2283	0.1384316776306415	No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2224	0.1348541616515754	No Hit
AGGGTCGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAATC	2101	0.12739595037318344	No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1898	0.11508686997063405	No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1875	0.1136922450974388	No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1852	0.11229762022424356	No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1781	0.10799247387655386	No Hit
AGGGTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1779	0.10787120214844995	No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1672	0.10138316469488942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.819075921559021E-4	0.0	0.0	0.0	0.0
8	6.063586405196736E-4	0.0	0.0	0.0	0.0
9	7.276303686236084E-4	0.0	0.0	0.0	0.0
10	0.004305146347689683	0.0	0.0	0.0	0.0
11	0.006063586405196736	0.0	0.0	0.0	0.0
12	0.008549656831327399	0.0	0.0	0.0	0.0
13	0.011096363121510027	0.0	0.0	0.0	0.0
14	0.015886596381615447	0.0	0.0	0.0	0.0
15	0.021707639330604316	0.0	0.0	0.0	0.0
16	0.03147001344297106	0.0	0.0	0.0	0.0
17	0.036017703246868614	0.0	0.0	0.0	0.0
18	0.04044412132266223	0.0	0.0	0.0	0.0
19	0.046143892543547164	0.0	0.0	0.0	0.0
20	0.05014585957097701	0.0	0.0	0.0	0.0
21	0.05475418523892653	0.0	0.0	0.0	0.0
22	0.05978696195523982	0.0	0.0	0.0	0.0
23	0.06330384207025393	0.0	0.0	0.0	0.0
24	0.06785153187415148	0.0	0.0	0.0	0.0
25	0.07148968371726952	0.0	0.0	0.0	0.0
26	0.07464274864797182	0.0	0.0	0.0	0.0
27	0.07828090049108986	0.0	0.0	0.0	0.0
28	0.08313176961524725	0.0	0.0	0.0	0.0
29	0.08725500837078103	0.0	0.0	0.0	0.0
30	0.0930760513197699	0.0	0.0	0.0	0.0
31	0.09913963772496663	0.0	0.0	0.0	0.0
32	0.10677975659551452	0.0	0.0	0.0	0.0
33	0.11047854430268453	0.0	0.0	0.0	0.0
34	0.11520814169873798	0.0	0.0	0.0	0.0
35	0.12472797235489687	0.0	0.0	0.0	0.0
36	0.13303508573001638	0.0	0.0	0.0	0.0
37	0.13934121559142099	0.0	0.0	0.0	0.0
38	0.1454654378606697	0.0	0.0	0.0	0.0
39	0.1526811056828538	0.0	0.0	0.0	0.0
40	0.1591691431364143	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGA	35	1.01992555E-7	46.000004	9
CTAGCGT	35	1.01992555E-7	46.000004	22
TAATACG	20	6.312258E-4	46.0	13
CGTATAT	60	0.0	46.0	46
GTCGACA	40	5.6134013E-9	46.0	10
TAGCGTA	45	3.110472E-10	46.0	40
TAGCGAT	25	3.4178072E-5	46.0	26
CCAATCG	30	1.8618994E-6	46.0	34
CGACCAA	25	3.4178072E-5	46.0	18
ATTCGTT	25	3.4178072E-5	46.0	39
TTATCGA	20	6.312258E-4	46.0	20
TACGATA	30	1.8618994E-6	46.0	38
AACGCGT	25	3.4178072E-5	46.0	35
TCGTATT	50	1.6370905E-11	46.0	43
ACGTCGT	60	0.0	46.0	16
CATCGTA	40	5.6134013E-9	46.0	38
TTACGAC	20	6.312258E-4	46.0	15
CCGGTTA	25	3.4178072E-5	46.0	24
ACGTAAA	20	6.312258E-4	46.0	11
TGTACGG	25	3.4178072E-5	46.0	39
>>END_MODULE