FastQCFastQC Report
Fri 17 Jun 2016
SRR1527064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604300
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG50890.3172099981300256No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT43010.2680920027426292No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC35460.2210309792432837No Hit
AGGGTCGGGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCCCAGCATAT33620.2095618026553637No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC32510.20264289721373807No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG31530.19653431403104157No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG30860.19235803777348376No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT25010.155893536121673No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC23450.14616966901452347No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC23410.1459203390886991No Hit
AGGGTCGGGACATAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGT22250.13868977123979306No Hit
AGGGTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC21860.13625880446300567No Hit
AGGGTCGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAATC18880.11768372498909181No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT18650.11625007791560182No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG18530.11550208813812878No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG17460.10883251262232749No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA17010.10602755095680358No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16780.1045939038833136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCATA351.01990736E-746.00000440
TAGCGTA351.01990736E-746.00000440
ACGATTG351.01990736E-746.00000441
CTATGCG253.4177654E-546.020
CGTATTG206.3122064E-446.021
ACGCATA206.3122064E-446.032
CACGCAT253.4177654E-546.025
ATCGTAC253.4177654E-546.025
GTAATCG206.3122064E-446.013
AACGATT301.8618666E-646.040
GTATACC206.3122064E-446.036
CGTAACG253.4177654E-546.046
CGCGTTA501.6370905E-1146.026
ATACGCT253.4177654E-546.037
CGTCATA453.110472E-1046.027
TATCGCA206.3122064E-446.019
TCGAATT253.4177654E-546.011
ATACACG253.4177654E-546.027
TGCGTAA453.110472E-1046.017
AGGGTCG1634000.044.8500021
Produced by FastQC (version 0.11.5)