##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1604300
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60399052546282	31.0	31.0	33.0	30.0	34.0
2	32.33928379978807	33.0	31.0	34.0	31.0	34.0
3	32.892422240229386	34.0	31.0	34.0	31.0	34.0
4	36.41376425855513	37.0	37.0	37.0	35.0	37.0
5	33.62276569220221	35.0	33.0	35.0	32.0	35.0
6	35.243111637474286	35.0	35.0	37.0	35.0	37.0
7	36.18621766502525	37.0	35.0	37.0	35.0	37.0
8	36.59545783207629	37.0	37.0	37.0	35.0	37.0
9	38.61031727233061	39.0	39.0	39.0	38.0	39.0
10	38.03625755781338	39.0	38.0	39.0	37.0	39.0
11	37.60223274948576	39.0	37.0	39.0	35.0	39.0
12	37.23180078538927	39.0	37.0	39.0	34.0	39.0
13	37.11566165929066	39.0	37.0	39.0	34.0	39.0
14	38.0129196534314	40.0	38.0	40.0	34.0	40.0
15	38.0875172972636	40.0	38.0	40.0	34.0	40.0
16	38.14368073302998	40.0	38.0	40.0	34.0	40.0
17	38.08477840802843	40.0	38.0	40.0	34.0	40.0
18	38.043295518294585	40.0	38.0	40.0	34.0	40.0
19	37.97694196845976	40.0	38.0	40.0	34.0	40.0
20	37.88480022439693	40.0	38.0	40.0	34.0	40.0
21	33.54065324440566	39.0	34.0	40.0	10.0	40.0
22	34.14691267219348	39.0	34.0	40.0	16.0	40.0
23	36.15455089447111	39.0	34.0	40.0	30.0	40.0
24	36.96953125973945	39.0	35.0	40.0	31.0	40.0
25	37.28629558062706	39.0	36.0	40.0	33.0	40.0
26	37.340400797855764	39.0	36.0	40.0	33.0	40.0
27	37.28088823786075	39.0	36.0	40.0	33.0	40.0
28	37.153005048931	39.0	36.0	40.0	33.0	40.0
29	37.046816680172036	39.0	35.0	40.0	32.0	40.0
30	36.9327183195163	39.0	35.0	40.0	32.0	40.0
31	36.802734525961476	39.0	35.0	40.0	32.0	40.0
32	36.694248581936044	38.0	35.0	40.0	31.0	40.0
33	36.324178146232	38.0	35.0	40.0	31.0	40.0
34	36.196109829832324	38.0	35.0	40.0	30.0	40.0
35	36.03805896652746	38.0	35.0	40.0	30.0	40.0
36	35.85207442498286	38.0	34.0	40.0	30.0	40.0
37	35.72767063516799	38.0	34.0	40.0	30.0	40.0
38	35.57949884684909	37.0	34.0	40.0	30.0	40.0
39	35.398782646637166	37.0	34.0	40.0	29.0	40.0
40	30.58266596023188	35.0	29.0	39.0	8.0	40.0
41	31.355786324253568	35.0	29.0	39.0	12.0	40.0
42	33.05034843857134	35.0	30.0	39.0	23.0	40.0
43	33.821898647385154	35.0	32.0	39.0	26.0	40.0
44	34.74106962538178	36.0	33.0	39.0	28.0	40.0
45	35.062907186935114	36.0	34.0	39.0	29.0	40.0
46	35.11118057719878	35.0	34.0	39.0	30.0	40.0
47	35.0481119491367	35.0	34.0	39.0	30.0	40.0
48	34.875735211618775	35.0	34.0	39.0	29.0	40.0
49	34.68391572648507	35.0	34.0	39.0	29.0	40.0
50	34.61552826778034	35.0	34.0	39.0	29.0	40.0
51	34.42951069002057	35.0	34.0	39.0	29.0	40.0
52	33.990801595711524	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	6.0
15	21.0
16	43.0
17	94.0
18	226.0
19	435.0
20	698.0
21	1346.0
22	2205.0
23	3495.0
24	5262.0
25	7499.0
26	10502.0
27	14144.0
28	19448.0
29	25849.0
30	35069.0
31	48900.0
32	67371.0
33	95688.0
34	152318.0
35	190980.0
36	236982.0
37	269731.0
38	355018.0
39	60963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.3374057221218	0.5346880259303123	0.1116374742878514	0.016268777660038647
8	99.50595275197905	0.299569905877953	0.08664214922396062	0.1078351929190301
9	96.53381537118992	1.6410895717758525	0.7765380539799289	1.0485570030542917
10	53.27220594651874	33.92538801969706	4.755906002617964	8.046500031166241
11	32.6231378171165	22.04949199027613	25.05161129464564	20.275758897961726
12	26.896652745745808	17.78844355793804	28.715763884560246	26.599139811755908
13	23.583743688836254	17.8422988219161	31.682291342018328	26.89166614722932
14	26.053730599015147	17.153150906937604	31.191485383033097	25.60163311101415
15	25.24141370067942	18.469488250327245	28.98217291030356	27.30692513868977
16	29.456211431777103	18.86442685283301	25.429720127158262	26.249641588231626
17	30.24465498971514	19.402605497724863	23.949947017390762	26.40279249516923
18	29.345321947266722	19.128342579318083	24.025369319952627	27.50096615346257
19	29.168422364894347	19.03403353487502	24.511874337717384	27.285669762513248
20	29.29664027924952	19.31683600324129	24.946144736021942	26.44037898148725
21	35.469862245215985	18.212242099357976	24.04282241476033	22.275073240665712
22	30.845103783581624	18.216231378171166	24.821043445739573	26.117621392507633
23	28.37854515988282	20.74468615595587	24.883001932306925	25.99376675185439
24	28.300193230692518	19.673003802281368	24.636913295518294	27.389889671507824
25	28.27046063703796	20.178956554260424	24.451598828149347	27.098983980552266
26	27.991335785077602	21.149971950383346	24.314654366390325	26.544037898148726
27	28.478713457582742	18.630742379854144	23.28878638658605	29.60175777597706
28	28.890294832637288	19.936233871470424	24.31664900579692	26.856822290095366
29	29.82708969644082	19.93486255687839	23.0614598267157	27.176587919965094
30	28.320451287165742	19.320575952128653	24.643146543663903	27.715826217041702
31	29.421928566976252	20.774543414573333	22.628311413077355	27.17521660537306
32	30.30393317957988	20.80327868852459	23.9073116000748	24.985476531820733
33	30.71807018637412	20.08365019011407	23.111575141806394	26.086704481705418
34	29.53618400548526	22.475347503584118	24.239917721124478	23.748550769806144
35	28.39200897587733	23.422489559309355	24.516798603752417	23.6687028610609
36	29.831764632550023	22.64401919840429	22.69943277441875	24.82478339462694
37	30.596459515053294	23.06738141245403	21.497974194352675	24.838184878139998
38	30.522096864676186	23.290781025992644	22.74400049865985	23.443121610671323
39	29.551891790812192	22.896216418375616	22.070685033971202	25.48120675684099
40	28.378919154771552	27.91921710403291	21.490992956429594	22.21087078476594
41	26.82603004425606	22.724365767001185	23.555382409773735	26.89422177896902
42	27.580190737393256	20.56036900829022	23.811007916225144	28.048432338091377
43	27.01358848095743	20.151218600012466	25.61272829271333	27.22246462631677
44	26.90313532381724	20.402106837873216	25.846537430655115	26.848220407654427
45	27.52166053730599	20.70853331671134	25.279062519478902	26.49074362650377
46	27.339151031602565	21.376799850402044	24.091317085333166	27.19273203266222
47	27.061148164308417	21.797855762637912	24.60144611356978	26.53954995948389
48	28.661409960730538	20.081842548151844	25.749735086953812	25.50701240416381
49	26.62120551019136	22.31889297512934	24.961353861497226	26.098547653182074
50	24.728230380851464	23.016144112697127	25.862681543352238	26.39294396309917
51	27.407654428722804	22.400486193355356	23.789440877641336	26.402418500280493
52	27.848906064950445	22.49635354983482	23.38359409088076	26.27114629433398
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	2.0
10	0.0
11	1.0
12	2.0
13	88.0
14	756.0
15	1338.0
16	1734.5
17	2131.0
18	2296.0
19	2461.0
20	3009.5
21	3558.0
22	3658.5
23	3759.0
24	3888.0
25	4017.0
26	3932.5
27	3848.0
28	5620.5
29	7393.0
30	8654.0
31	9915.0
32	9685.5
33	9456.0
34	10961.5
35	12467.0
36	14826.5
37	17186.0
38	19789.0
39	23800.5
40	25209.0
41	28837.5
42	32466.0
43	35885.5
44	39305.0
45	40072.5
46	40840.0
47	45108.0
48	49376.0
49	51112.5
50	52849.0
51	59224.0
52	65599.0
53	69678.0
54	73757.0
55	78729.5
56	83702.0
57	92850.5
58	101999.0
59	113988.5
60	125978.0
61	139734.0
62	153490.0
63	144952.5
64	135367.0
65	134319.0
66	125246.0
67	116173.0
68	103213.5
69	90254.0
70	78381.5
71	66509.0
72	54749.5
73	42990.0
74	36448.5
75	29907.0
76	26920.5
77	23934.0
78	18485.5
79	13037.0
80	8475.0
81	3913.0
82	2772.5
83	1632.0
84	998.0
85	364.0
86	248.0
87	132.0
88	81.0
89	20.5
90	11.0
91	6.5
92	2.0
93	3.0
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1604300.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.609015089973168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72437407839364	6.988096079281379
2	8.786311909524407	1.6885560744766197
3	3.640209343302244	1.0493647953135776
4	1.9517285421409774	0.7501675605185594
5	1.1074786561404295	0.5320889559338295
6	0.7086418536377558	0.40856101589950505
7	0.47399929427470855	0.3188266459925615
8	0.32587128150724526	0.25050416491136107
9	0.2526001145892779	0.218451448153559
>10	4.220761045110338	11.31533672607247
>50	3.142400980596853	21.80479726477186
>100	2.531108708984909	42.265846332701685
>500	0.10013834278239675	6.552289409354224
>1k	0.033628547949312346	5.4916812296205135
>5k	7.473010655402743E-4	0.3654322969982974
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5089	0.3172099981300256	No Hit
AGGGTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4301	0.2680920027426292	No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3546	0.2210309792432837	No Hit
AGGGTCGGGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCCCAGCATAT	3362	0.2095618026553637	No Hit
AGGGTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3251	0.20264289721373807	No Hit
AGGGTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3153	0.19653431403104157	No Hit
AGGGTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3086	0.19235803777348376	No Hit
AGGGTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2501	0.155893536121673	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2345	0.14616966901452347	No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2341	0.1459203390886991	No Hit
AGGGTCGGGACATAGTATAAAAATTCGCCATTTTAAAAAGTGTAAAATTTGT	2225	0.13868977123979306	No Hit
AGGGTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	2186	0.13625880446300567	No Hit
AGGGTCGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAATC	1888	0.11768372498909181	No Hit
AGGGTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1865	0.11625007791560182	No Hit
AGGGTCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1853	0.11550208813812878	No Hit
AGGGTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1746	0.10883251262232749	No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1701	0.10602755095680358	No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1678	0.1045939038833136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.986598516486941E-4	0.0	0.0	0.0	0.0
8	6.856572960169544E-4	0.0	0.0	0.0	0.0
9	9.349872218413015E-4	0.0	0.0	0.0	0.0
10	0.006420245589976937	0.0	0.0	0.0	0.0
11	0.009287539736956929	0.0	0.0	0.0	0.0
12	0.01234183132830518	0.0	0.0	0.0	0.0
13	0.014772798105092563	0.0	0.0	0.0	0.0
14	0.018824409399738205	0.0	0.0	0.0	0.0
15	0.026615969581749048	0.0	0.0	0.0	0.0
16	0.03384653743065511	0.0	0.0	0.0	0.0
17	0.03640216917035467	0.0	0.0	0.0	0.0
18	0.04039144798354422	0.0	0.0	0.0	0.0
19	0.04512871657420682	0.0	0.0	0.0	0.0
20	0.04855700305429159	0.0	0.0	0.0	0.0
21	0.052172286978744625	0.0	0.0	0.0	0.0
22	0.056971888050863305	0.0	0.0	0.0	0.0
23	0.06127282927133329	0.0	0.0	0.0	0.0
24	0.06613476282490806	0.0	0.0	0.0	0.0
25	0.07049803652683413	0.0	0.0	0.0	0.0
26	0.07498597519167238	0.0	0.0	0.0	0.0
27	0.07841426167175715	0.0	0.0	0.0	0.0
28	0.08377485507698061	0.0	0.0	0.0	0.0
29	0.08726547403852147	0.0	0.0	0.0	0.0
30	0.09256373496228885	0.0	0.0	0.0	0.0
31	0.09854765318207317	0.0	0.0	0.0	0.0
32	0.10571588854952316	0.0	0.0	0.0	0.0
33	0.11051548962164184	0.0	0.0	0.0	0.0
34	0.11519042573084834	0.0	0.0	0.0	0.0
35	0.12316898335722745	0.0	0.0	0.0	0.0
36	0.13039955120613353	0.0	0.0	0.0	0.0
37	0.1361341395000935	0.0	0.0	0.0	0.0
38	0.14105840553512436	0.0	0.0	0.0	0.0
39	0.14722932119927695	0.0	0.0	0.0	0.0
40	0.154459889048183	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCATA	35	1.01990736E-7	46.000004	40
TAGCGTA	35	1.01990736E-7	46.000004	40
ACGATTG	35	1.01990736E-7	46.000004	41
CTATGCG	25	3.4177654E-5	46.0	20
CGTATTG	20	6.3122064E-4	46.0	21
ACGCATA	20	6.3122064E-4	46.0	32
CACGCAT	25	3.4177654E-5	46.0	25
ATCGTAC	25	3.4177654E-5	46.0	25
GTAATCG	20	6.3122064E-4	46.0	13
AACGATT	30	1.8618666E-6	46.0	40
GTATACC	20	6.3122064E-4	46.0	36
CGTAACG	25	3.4177654E-5	46.0	46
CGCGTTA	50	1.6370905E-11	46.0	26
ATACGCT	25	3.4177654E-5	46.0	37
CGTCATA	45	3.110472E-10	46.0	27
TATCGCA	20	6.3122064E-4	46.0	19
TCGAATT	25	3.4177654E-5	46.0	11
ATACACG	25	3.4177654E-5	46.0	27
TGCGTAA	45	3.110472E-10	46.0	17
AGGGTCG	163400	0.0	44.850002	1
>>END_MODULE