FastQCFastQC Report
Fri 17 Jun 2016
SRR1527063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435517
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC65220.2677870858630837No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG54930.22553732944586305No Hit
GGGTTTGGGAGCTGAACCCACCTCACCCACCCTCTCCTTCGCAGGCAGGG46280.19002125626714986No Hit
GGGTTTGGGGACTGGAGTTCAGATTTCTTCCAACGAAAGTTTTCTGTCTC41960.1722837491998619No Hit
GGGTTTGGGAGCTTCTTTGGGTCTGTGGATTGTACCATAGTTATCCTGCA39100.16054086257661104No Hit
GGGTTTGGGATCTTGACTTGCAAAGTACGGTGTAGAGACCTACTGTGTGC36700.15068669198367327No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35150.14432254014240098No Hit
GGGTTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACT29190.11985134983660553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC302.5288882E-644.00000440
CGAATAT600.044.00000432
CGGTATG302.5288882E-644.00000423
CGCGTAA302.5288882E-644.00000414
TCGTTAC502.7284841E-1144.026
CGACGTA408.3182385E-944.042
CCGATAG254.4443113E-544.021
CGTGATA408.3182385E-944.015
CTATACG254.4443113E-544.037
ACCGATA254.4443113E-544.038
CGCGCAA207.8582205E-444.041
CGCGAAT254.4443113E-544.013
CGCGAAA800.044.012
TACGCCG207.8582205E-444.028
ACTACGG254.4443113E-544.040
CGTCGAA454.802132E-1044.025
ATCGGTC207.8582205E-444.016
GCGTCGT207.8582205E-444.044
TATCGGG254.4443113E-544.041
CGATATT351.4469697E-744.031
Produced by FastQC (version 0.11.5)