##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2435517
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80127217342355	31.0	31.0	33.0	31.0	33.0
2	32.64840606737707	34.0	31.0	34.0	31.0	34.0
3	33.160829097066454	34.0	34.0	34.0	31.0	34.0
4	33.318837027210236	35.0	33.0	35.0	32.0	35.0
5	35.0309884923817	35.0	35.0	35.0	35.0	37.0
6	35.84978014934817	37.0	35.0	37.0	35.0	37.0
7	36.142402619238545	37.0	35.0	37.0	35.0	37.0
8	36.61081569128854	37.0	37.0	37.0	35.0	37.0
9	38.747593632070725	39.0	39.0	39.0	39.0	39.0
10	38.135219750057175	39.0	39.0	39.0	37.0	39.0
11	37.90118894674108	39.0	38.0	39.0	35.0	39.0
12	37.64300228657817	39.0	37.0	39.0	35.0	39.0
13	37.526188074236394	39.0	37.0	39.0	35.0	39.0
14	38.35258181322487	40.0	38.0	40.0	35.0	40.0
15	38.29688809398579	40.0	38.0	40.0	35.0	40.0
16	38.145916041645364	40.0	38.0	40.0	35.0	40.0
17	38.27251667715725	40.0	38.0	40.0	35.0	40.0
18	38.24929409238367	40.0	38.0	40.0	35.0	40.0
19	38.238321473428435	40.0	38.0	40.0	34.0	40.0
20	37.97711081466481	40.0	38.0	40.0	34.0	40.0
21	38.173497043954114	40.0	38.0	40.0	34.0	40.0
22	38.01907069423042	40.0	38.0	40.0	34.0	40.0
23	38.08847115417384	40.0	38.0	40.0	34.0	40.0
24	38.15163720885545	40.0	38.0	40.0	34.0	40.0
25	38.09907095700831	40.0	38.0	40.0	34.0	40.0
26	37.97209052533815	40.0	38.0	40.0	34.0	40.0
27	37.82964191997017	40.0	38.0	40.0	34.0	40.0
28	37.763309802395135	40.0	37.0	40.0	34.0	40.0
29	38.03601822528851	40.0	38.0	40.0	34.0	40.0
30	38.06804222676335	40.0	38.0	40.0	34.0	40.0
31	38.04010154722796	40.0	38.0	40.0	34.0	40.0
32	37.329037736135696	40.0	37.0	40.0	33.0	40.0
33	37.567054962047074	40.0	37.0	40.0	33.0	40.0
34	37.597709233809496	40.0	37.0	40.0	33.0	40.0
35	37.6692086320892	40.0	37.0	40.0	33.0	40.0
36	37.495086669483314	40.0	37.0	40.0	33.0	40.0
37	37.52846931472866	40.0	37.0	40.0	33.0	40.0
38	37.35282282981396	40.0	37.0	40.0	33.0	40.0
39	37.40196352560873	40.0	37.0	40.0	33.0	40.0
40	37.33917890944715	40.0	36.0	40.0	33.0	40.0
41	37.26467727386013	40.0	36.0	40.0	33.0	40.0
42	37.177197285011765	40.0	36.0	40.0	33.0	40.0
43	37.11703757354188	40.0	36.0	40.0	33.0	40.0
44	37.00730686749466	40.0	35.0	40.0	33.0	40.0
45	36.92159529167729	40.0	35.0	40.0	32.0	40.0
46	36.642612636249304	40.0	35.0	40.0	31.0	40.0
47	36.647560251067844	39.0	35.0	40.0	31.0	40.0
48	36.579483124116976	39.0	35.0	40.0	31.0	40.0
49	36.34165312744686	39.0	35.0	40.0	31.0	40.0
50	35.89883544233113	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	7.0
15	12.0
16	35.0
17	84.0
18	161.0
19	361.0
20	661.0
21	1074.0
22	1719.0
23	2760.0
24	4078.0
25	6073.0
26	8539.0
27	12551.0
28	16665.0
29	22734.0
30	30345.0
31	38636.0
32	49497.0
33	68931.0
34	123443.0
35	137158.0
36	212303.0
37	366553.0
38	1044570.0
39	286560.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.71238139581862	0.12112418020485999	0.13734250263907005	0.02915192133744088
8	99.53369243573336	0.23629479900982006	0.11751098432078282	0.11250178093603945
9	97.68287390315896	1.0624027670511025	0.4860159054525179	0.76870742433742
10	39.15238530463963	47.65103261443053	4.247886588350646	8.948695492579194
11	28.079910754061665	22.345563590810492	32.62629659329005	16.948229061837793
12	26.990121604571023	18.33586051749998	28.846811580457043	25.827206297471957
13	23.87957053882194	18.66589311427512	32.54951618075341	24.905020166149527
14	25.662559530481623	22.008345661311335	30.07940408545701	22.24969072275004
15	26.167216241972447	23.18234691032746	29.599177505227843	21.051259342472257
16	28.842828853175735	25.70062126439684	26.121599643935966	19.33495023849146
17	27.4785189345835	23.2016857201161	25.8021192215041	23.517676123796303
18	25.160284243550752	23.402341268814794	28.232034512590136	23.20533997504431
19	25.6592337479065	24.256903154443187	27.99861384666993	22.08524925098039
20	25.81805013062935	25.462643044577394	26.911657771224757	21.807649053568504
21	25.418586690218138	24.102890679884396	29.351221937683047	21.127300692214426
22	25.295286380674	23.239131568369263	29.520385199528477	21.945196851428257
23	23.348594980039145	25.8854690811027	29.12428860073652	21.64164733812164
24	24.075668533621403	24.68666816942768	28.549503041859285	22.68816025509163
25	24.193056340809775	26.948816206168956	27.015249739583012	21.842877713438256
26	24.51196193662372	26.699423572079358	27.092194388296203	21.69642010300072
27	22.139200834976723	25.219039735711146	27.758664792731892	24.883094636580243
28	23.64569822341622	25.965862689523416	27.242265194617815	23.146173892442548
29	25.244783756385196	25.764509137074388	27.175954838336175	21.814752268204245
30	24.351954841620895	25.001303624651356	27.793113330763035	22.85362820296471
31	25.139138835820074	25.875984441906997	26.582076823935125	22.402799898337808
32	26.341142352937798	25.42564884580974	27.130379299343836	21.10282950190863
33	25.3205376928184	26.18318821014183	27.362198662542696	21.13407543449707
34	23.41494639536493	27.18621959937048	28.773685422848622	20.62514858241597
35	23.63350368730746	28.920717859904077	27.551973564545023	19.89380488824344
36	23.15241486715141	27.573570621761213	27.412578109699094	21.861436401388286
37	24.363081842582087	28.11908108216859	25.942500093409326	21.575336981839996
38	23.43186272154947	28.049855533753203	26.773247733438115	21.74503401125921
39	24.801920906320916	26.630403318884653	26.160441499689803	22.407234275104628
40	23.299200949942044	27.718878578962908	26.02334535131555	22.958575119779496
41	22.442093403577147	28.567158430838298	26.58092717069928	22.409820994885273
42	22.085002896715565	27.775950650313668	26.87408874583918	23.264957707131586
43	22.880603994962875	27.74918015353619	26.46013146284752	22.910084388653416
44	22.93250262675235	27.646984192678598	26.719378267530058	22.701134913039
45	23.21268954394488	27.49280748194326	25.715361461242107	23.579141512869754
46	22.65748914912111	28.606492995121773	25.709161545577388	23.026856310179728
47	22.523143956704057	28.52215771846388	27.143189721114652	21.811508603717403
48	22.24841789238178	28.10885738017842	27.052408174527216	22.590316552912583
49	23.582672590665553	28.181449770213057	25.949931780398167	22.28594585872322
50	22.273956617835143	28.90043469210028	26.19238543602857	22.633223254036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	45.5
6	91.0
7	79.0
8	67.0
9	107.0
10	147.0
11	2046.0
12	3945.0
13	9305.5
14	14666.0
15	18102.0
16	21538.0
17	18600.5
18	15663.0
19	15248.0
20	14833.0
21	14475.5
22	14118.0
23	15718.5
24	17319.0
25	18372.0
26	19425.0
27	24907.0
28	30389.0
29	32825.0
30	35261.0
31	40395.0
32	45529.0
33	50937.5
34	56346.0
35	61375.5
36	66405.0
37	73621.0
38	80837.0
39	84870.0
40	88903.0
41	96269.5
42	103636.0
43	110069.5
44	116503.0
45	120075.0
46	123647.0
47	129573.0
48	135499.0
49	140535.5
50	145572.0
51	147274.0
52	148976.0
53	158054.0
54	167132.0
55	166012.0
56	164892.0
57	167977.0
58	171062.0
59	166260.0
60	161458.0
61	151267.5
62	141077.0
63	128761.0
64	116445.0
65	99631.5
66	82818.0
67	69451.5
68	56085.0
69	42697.0
70	29309.0
71	23272.5
72	17236.0
73	16232.5
74	15229.0
75	11023.5
76	6818.0
77	4858.5
78	2899.0
79	2310.0
80	1721.0
81	1776.5
82	1832.0
83	985.0
84	138.0
85	78.5
86	19.0
87	16.0
88	13.0
89	9.5
90	6.0
91	7.5
92	9.0
93	6.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2435517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.0667521586930775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.46379316491982	4.696831538231273
2	10.950936015576621	1.5477510145557172
3	4.710592392034255	0.9986576686539378
4	2.48830049607229	0.7033681160838365
5	1.4383583845996857	0.5082261109672059
6	0.8492698031554073	0.36009475288567916
7	0.5541572836583142	0.27412645263835184
8	0.4023335466033405	0.22745531671790387
9	0.29676373001606565	0.18874401567415577
>10	4.116569959080367	7.940914590333005
>50	3.521779913979053	18.266839231437114
>100	4.025152189966033	52.538185583355116
>500	0.14303807116921485	6.8221812835638165
>1k	0.03773770388294179	4.409818478200886
>5k	0.0012173452865465095	0.5168058467020116
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6522	0.2677870858630837	No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5493	0.22553732944586305	No Hit
GGGTTTGGGAGCTGAACCCACCTCACCCACCCTCTCCTTCGCAGGCAGGG	4628	0.19002125626714986	No Hit
GGGTTTGGGGACTGGAGTTCAGATTTCTTCCAACGAAAGTTTTCTGTCTC	4196	0.1722837491998619	No Hit
GGGTTTGGGAGCTTCTTTGGGTCTGTGGATTGTACCATAGTTATCCTGCA	3910	0.16054086257661104	No Hit
GGGTTTGGGATCTTGACTTGCAAAGTACGGTGTAGAGACCTACTGTGTGC	3670	0.15068669198367327	No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3515	0.14432254014240098	No Hit
GGGTTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACT	2919	0.11985134983660553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.211808827448135E-5	0.0	0.0	0.0	0.0
8	1.642361765489627E-4	0.0	0.0	0.0	0.0
9	4.105904413724068E-4	0.0	0.0	0.0	0.0
10	0.011455473314290148	0.0	0.0	0.0	0.0
11	0.01831233368520934	0.0	0.0	0.0	0.0
12	0.023937422732011313	0.0	0.0	0.0	0.0
13	0.02964462986708777	0.0	0.0	0.0	0.0
14	0.04036104038690758	0.0	0.0	0.0	0.0
15	0.052432399363256346	0.0	0.0	0.0	0.0
16	0.06721365525266298	0.0	0.0	0.0	0.0
17	0.07431686988840562	0.0	0.0	0.0	0.0
18	0.08006513606761932	0.0	0.0	0.0	0.0
19	0.08991930666055709	0.0	0.0	0.0	0.0
20	0.09735099364939764	0.0	0.0	0.0	0.0
21	0.10387938166721891	0.0	0.0	0.0	0.0
22	0.11213224953880428	0.0	0.0	0.0	0.0
23	0.11853746042421383	0.0	0.0	0.0	0.0
24	0.1270777416047599	0.0	0.0	0.0	0.0
25	0.13393460197567908	0.0	0.0	0.0	0.0
26	0.14087358043487275	0.0	0.0	0.0	0.0
27	0.14801785411475263	0.0	0.0	0.0	0.0
28	0.16103357110625793	0.0	0.0	0.0	0.0
29	0.16862949427164745	0.0	0.0	0.0	0.0
30	0.1779498972908011	0.0	0.0	0.0	0.0
31	0.18649017847134716	0.0	0.0	0.0	0.0
32	0.19597481766704974	0.0	0.0	0.0	0.0
33	0.20398133127381168	0.0	0.0	0.0	0.0
34	0.21190572679229913	0.0	0.0	0.0	0.0
35	0.2318604222429981	0.0	0.0	0.0	0.0
36	0.2421662423214455	0.0	0.0	0.0	0.0
37	0.2511581729875012	0.0	0.0	0.0	0.0
38	0.2605606940949293	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCCC	30	2.5288882E-6	44.000004	40
CGAATAT	60	0.0	44.000004	32
CGGTATG	30	2.5288882E-6	44.000004	23
CGCGTAA	30	2.5288882E-6	44.000004	14
TCGTTAC	50	2.7284841E-11	44.0	26
CGACGTA	40	8.3182385E-9	44.0	42
CCGATAG	25	4.4443113E-5	44.0	21
CGTGATA	40	8.3182385E-9	44.0	15
CTATACG	25	4.4443113E-5	44.0	37
ACCGATA	25	4.4443113E-5	44.0	38
CGCGCAA	20	7.8582205E-4	44.0	41
CGCGAAT	25	4.4443113E-5	44.0	13
CGCGAAA	80	0.0	44.0	12
TACGCCG	20	7.8582205E-4	44.0	28
ACTACGG	25	4.4443113E-5	44.0	40
CGTCGAA	45	4.802132E-10	44.0	25
ATCGGTC	20	7.8582205E-4	44.0	16
GCGTCGT	20	7.8582205E-4	44.0	44
TATCGGG	25	4.4443113E-5	44.0	41
CGATATT	35	1.4469697E-7	44.0	31
>>END_MODULE