FastQCFastQC Report
Fri 17 Jun 2016
SRR1527062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581562
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC62970.24392209057926945No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT52180.20212568979555787No Hit
GGGTTTGGGAGCTGAACCCACCTCACCCACCCTCTCCTTCGCAGGCAGGGAC48160.18655372212637156No Hit
GGGTTTGGGGACTGGAGTTCAGATTTCTTCCAACGAAAGTTTTCTGTCTCTA44300.17160153426491404No Hit
GGGTTTGGGAGCTTCTTTGGGTCTGTGGATTGTACCATAGTTATCCTGCACT38060.1474301217634905No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35130.13608040403445668No Hit
GGGTTTGGGATCTTGACTTGCAAAGTACGGTGTAGAGACCTACTGTGTGCTT30210.11702217494679577No Hit
GGGTTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGC27820.10776421406884669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC253.418348E-546.014
ATCGTTC206.312923E-446.041
CGAATAT253.418348E-546.032
CGCGTAC253.418348E-546.038
TCGCGTA253.418348E-546.037
ACGACCG301.8623123E-646.031
TTGGGAT470450.044.151985
GTTTGGG2617000.044.0568243
TTTGGGA1256400.044.044894
GGTTTGG2679550.043.994032
TTGGGAC210750.043.915545
GGGTTTG2703000.043.803811
TTTGGGG1044700.043.4043274
TGGGATC134800.043.3724066
TTTGGGC234300.043.3495524
CGATTTA850.043.29411729
TTGGGAG371100.042.963085
TTGGGGT284700.042.9058655
CGCGAAA1450.042.82758312
TTGGGGC196900.042.7293055