##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2581562
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.780428670704016	31.0	31.0	33.0	31.0	33.0
2	32.57855282964345	34.0	31.0	34.0	31.0	34.0
3	33.09211438656131	34.0	31.0	34.0	31.0	34.0
4	33.17755529404291	35.0	33.0	35.0	30.0	35.0
5	34.87846389124104	35.0	35.0	35.0	33.0	37.0
6	35.916277432035336	37.0	35.0	37.0	35.0	37.0
7	36.132372183972336	37.0	35.0	37.0	35.0	37.0
8	36.56077173432209	37.0	37.0	37.0	35.0	37.0
9	38.709699011683625	39.0	39.0	39.0	39.0	39.0
10	38.06355105939737	39.0	38.0	39.0	37.0	39.0
11	37.8537280917522	39.0	38.0	39.0	35.0	39.0
12	37.630723182321404	39.0	37.0	39.0	35.0	39.0
13	37.55818337890006	39.0	37.0	39.0	35.0	39.0
14	38.401305101330124	40.0	38.0	40.0	35.0	40.0
15	38.26755080838655	40.0	38.0	40.0	35.0	40.0
16	33.14256020192426	40.0	35.0	40.0	3.0	40.0
17	34.937960428608726	40.0	35.0	40.0	17.0	40.0
18	37.034405139214165	40.0	35.0	40.0	32.0	40.0
19	37.88983258972669	40.0	37.0	40.0	34.0	40.0
20	38.1223170313167	40.0	38.0	40.0	34.0	40.0
21	38.32871765233607	40.0	38.0	40.0	35.0	40.0
22	38.372563200109084	40.0	38.0	40.0	35.0	40.0
23	38.33010053603206	40.0	38.0	40.0	35.0	40.0
24	38.324485330974035	40.0	38.0	40.0	35.0	40.0
25	38.2536278423683	40.0	38.0	40.0	35.0	40.0
26	38.16192057366819	40.0	38.0	40.0	35.0	40.0
27	38.036029349672795	40.0	38.0	40.0	34.0	40.0
28	38.00825004396563	40.0	38.0	40.0	34.0	40.0
29	37.82876181164737	40.0	38.0	40.0	34.0	40.0
30	37.81461495017358	40.0	37.0	40.0	34.0	40.0
31	37.77546927015504	40.0	37.0	40.0	34.0	40.0
32	37.73294966380819	40.0	37.0	40.0	34.0	40.0
33	37.3916260775453	40.0	37.0	40.0	33.0	40.0
34	37.365220358837014	40.0	37.0	40.0	33.0	40.0
35	37.369140466120896	40.0	36.0	40.0	33.0	40.0
36	37.34345446671434	40.0	36.0	40.0	33.0	40.0
37	37.09944676904912	39.0	36.0	40.0	33.0	40.0
38	37.11952492328288	39.0	35.0	40.0	33.0	40.0
39	36.80801119632223	39.0	35.0	40.0	32.0	40.0
40	36.83320059715785	39.0	35.0	40.0	32.0	40.0
41	36.824187836666326	39.0	35.0	40.0	32.0	40.0
42	36.779201506684714	39.0	35.0	40.0	32.0	40.0
43	36.73491514052345	39.0	35.0	40.0	32.0	40.0
44	36.97401340738669	39.0	35.0	40.0	33.0	40.0
45	37.01976981377941	40.0	35.0	40.0	33.0	40.0
46	36.97135803827295	40.0	35.0	40.0	33.0	40.0
47	36.7226074756291	39.0	35.0	40.0	32.0	40.0
48	36.71701241341482	39.0	35.0	40.0	32.0	40.0
49	36.69541696073927	39.0	35.0	40.0	32.0	40.0
50	36.19789801678209	39.0	35.0	40.0	31.0	40.0
51	36.29697369267134	39.0	35.0	40.0	31.0	40.0
52	35.992541337376366	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	11.0
16	31.0
17	52.0
18	135.0
19	318.0
20	553.0
21	976.0
22	1647.0
23	2696.0
24	4364.0
25	6466.0
26	9507.0
27	13795.0
28	19379.0
29	26014.0
30	34201.0
31	45151.0
32	61604.0
33	88913.0
34	141118.0
35	177622.0
36	294286.0
37	504843.0
38	894213.0
39	253659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.71191859812005	0.11361338600428732	0.14603561719610064	0.028432398679559122
8	99.50832093128113	0.24934516389689654	0.12535046611315165	0.11698343870881273
9	97.65843315016257	1.0783006567341789	0.48544253440358975	0.7778236586996554
10	39.20823904287404	47.59908923357254	4.238906522485224	8.95376520106819
11	28.1314568466688	22.45710155324567	32.38895676338589	17.022484836699643
12	27.152902002740976	18.275214773071497	28.746123470983846	25.82575975320368
13	23.948407979355135	18.587816213594717	32.52747755041327	24.936298256636874
14	25.732599100854443	21.822369557655406	30.053703920339704	22.39132742115045
15	26.211611419752845	23.22888235881997	29.51364329038001	21.04586293104717
16	27.472359757387192	32.71023512121731	22.939174034944735	16.87823108645076
17	27.501450672112465	23.16880245370826	25.749410628139085	23.580336246040186
18	25.29642906116529	23.246739764530155	28.167713965420937	23.289117208883614
19	25.707846644783274	24.05400296409693	28.130023605863425	22.108126785256367
20	25.859770170152803	25.41941661676148	26.897204095814857	21.823609117270863
21	25.58195387133836	24.06721202124915	29.260734392588674	21.090099714823815
22	25.143304712418296	23.190804636882632	29.61269185090267	22.0531987997964
23	23.45413358269141	25.754407602838903	29.08374852124411	21.707710293225574
24	24.16269684787737	24.64562152681206	28.489379685632187	22.70230193967838
25	24.256361071320388	26.837511553082976	26.992766394919045	21.91336098067759
26	24.524803200542927	26.577668868692676	27.032199885185793	21.865328045578607
27	22.160188289105587	25.188393693430566	27.698346969780314	24.953071047683533
28	23.72613944580839	25.981905528513355	27.17606627305484	23.115888752623412
29	25.561423665207343	25.69180984225829	26.991759252731484	21.75500723980288
30	24.46445214176533	24.918634532116602	27.652328319056448	22.964585007061615
31	25.250642827869328	25.812589432289446	26.518712314482475	22.418055425358755
32	26.24046991705022	25.161355799318397	27.36808180473682	21.230092478894562
33	25.680576333243206	25.99356513614626	27.26353269842057	21.06232583218997
34	23.573944766772982	26.99621392009954	28.684261698924914	20.745579614202565
35	23.6952666641359	28.913425282832645	27.476620743565327	19.914687309466128
36	23.241161746260598	27.44233917295033	27.37451202024201	21.941987060547063
37	24.537005115507586	28.12913267238982	25.81049767543836	21.523364536664236
38	23.46505720180263	28.035933283802596	26.744273428257774	21.754736086136997
39	25.1771214481775	26.54966256863093	26.024786543960595	22.248429439230975
40	23.337033935268646	27.759472753317567	26.012119794140137	22.89137351727365
41	22.445209528184872	28.52052362097056	26.53680213762056	22.49746471322401
42	22.139464401784657	27.695635433121495	26.82949315182049	23.33540701327336
43	22.937585849187432	27.68974752494807	26.42295633418837	22.949710291676126
44	22.997936907965023	27.50997264446874	26.630776250967436	22.861314196598805
45	23.203277705513173	27.362116424087436	25.731204596287053	23.70340127411234
46	22.33535355726494	28.569873588160966	25.788572964739952	23.30619988983414
47	22.700984907586957	28.352020985744293	27.099523466800335	21.847470639868423
48	22.31184066080923	28.089234347267272	26.95348784960423	22.645437142319263
49	23.571814273683916	28.01412478181814	25.976676136385645	22.4373848081123
50	22.448618317127384	28.76037840656161	26.036601096545425	22.754402179765584
51	21.232222971983628	29.089055385847796	26.45987196898622	23.21884967318236
52	21.487533516529915	28.147493649193784	27.499087761595497	22.865885072680804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	40.0
6	80.0
7	82.0
8	84.0
9	111.5
10	139.0
11	1904.5
12	3670.0
13	9523.5
14	18874.0
15	22371.0
16	19252.5
17	16134.0
18	15793.0
19	15452.0
20	14505.5
21	13559.0
22	14468.0
23	15377.0
24	16572.5
25	17768.0
26	22530.0
27	27292.0
28	29967.0
29	32642.0
30	36727.0
31	40812.0
32	46393.5
33	51975.0
34	57215.0
35	62455.0
36	64974.0
37	67493.0
38	74449.0
39	94734.5
40	108064.0
41	106441.5
42	104819.0
43	111864.0
44	118909.0
45	118743.0
46	118577.0
47	129823.0
48	141069.0
49	145278.5
50	149488.0
51	150668.0
52	151848.0
53	161034.5
54	170221.0
55	171670.5
56	173120.0
57	175454.0
58	177788.0
59	172984.5
60	168181.0
61	156371.5
62	144562.0
63	135530.0
64	109462.5
65	92427.0
66	75392.5
67	58358.0
68	48078.0
69	37798.0
70	29113.0
71	20428.0
72	18428.0
73	16428.0
74	13787.5
75	11147.0
76	7121.5
77	3096.0
78	2454.0
79	1812.0
80	1889.5
81	1967.0
82	1393.0
83	819.0
84	422.5
85	26.0
86	19.0
87	12.0
88	9.5
89	5.0
90	3.0
91	2.0
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2581562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.805861502104464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.20509920551768	5.4020541963767075
2	10.098579106551501	1.5765621974757373
3	4.168057197908767	0.9760583165917636
4	2.203431300999314	0.6879871826001
5	1.239123733965577	0.4836214125652915
6	0.7759584570693083	0.3634214548361816
7	0.49294619882271007	0.26935088291992476
8	0.3374472119646109	0.21072529606936327
9	0.2691021070694446	0.18905163999377142
>10	4.065829025739944	8.722180876648935
>50	3.3820246188311254	19.30194945024116
>100	3.613583558924369	51.21646558787748
>500	0.11779052783900393	6.163902133595713
>1k	0.029878572915259534	3.9202039022680784
>5k	0.0011491758813561358	0.5164654699397845
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6297	0.24392209057926945	No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5218	0.20212568979555787	No Hit
GGGTTTGGGAGCTGAACCCACCTCACCCACCCTCTCCTTCGCAGGCAGGGAC	4816	0.18655372212637156	No Hit
GGGTTTGGGGACTGGAGTTCAGATTTCTTCCAACGAAAGTTTTCTGTCTCTA	4430	0.17160153426491404	No Hit
GGGTTTGGGAGCTTCTTTGGGTCTGTGGATTGTACCATAGTTATCCTGCACT	3806	0.1474301217634905	No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3513	0.13608040403445668	No Hit
GGGTTTGGGATCTTGACTTGCAAAGTACGGTGTAGAGACCTACTGTGTGCTT	3021	0.11702217494679577	No Hit
GGGTTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGC	2782	0.10776421406884669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	7.747247596610115E-5	0.0	0.0	0.0	0.0
9	4.260986178135563E-4	0.0	0.0	0.0	0.0
10	0.009335433353915188	0.0	0.0	0.0	0.0
11	0.015920593811033786	0.0	0.0	0.0	0.0
12	0.021188722176728663	0.0	0.0	0.0	0.0
13	0.025410972116881175	0.0	0.0	0.0	0.0
14	0.03652827241801669	0.0	0.0	0.0	0.0
15	0.04745189152923695	0.0	0.0	0.0	0.0
16	0.0637985839580843	0.0	0.0	0.0	0.0
17	0.0713134141267961	0.0	0.0	0.0	0.0
18	0.0778211021079486	0.0	0.0	0.0	0.0
19	0.0880087326974909	0.0	0.0	0.0	0.0
20	0.09509746424838915	0.0	0.0	0.0	0.0
21	0.10121778984971114	0.0	0.0	0.0	0.0
22	0.10873262001842296	0.0	0.0	0.0	0.0
23	0.11586008780730427	0.0	0.0	0.0	0.0
24	0.1250793124472703	0.0	0.0	0.0	0.0
25	0.13193562657027025	0.0	0.0	0.0	0.0
26	0.1389468856452024	0.0	0.0	0.0	0.0
27	0.14739138552550743	0.0	0.0	0.0	0.0
28	0.1596320367281514	0.0	0.0	0.0	0.0
29	0.16761170175265983	0.0	0.0	0.0	0.0
30	0.1758625204430496	0.0	0.0	0.0	0.0
31	0.1831449331838631	0.0	0.0	0.0	0.0
32	0.19131827939828677	0.0	0.0	0.0	0.0
33	0.1974773412375918	0.0	0.0	0.0	0.0
34	0.20534079754815107	0.0	0.0	0.0	0.0
35	0.22238474226069332	0.0	0.0	0.0	0.0
36	0.2320300655184729	0.0	0.0	0.0	0.0
37	0.23954489568718473	0.0	0.0	0.0	0.0
38	0.2501586248945406	0.0	0.0	0.0	0.0
39	0.2585256522988795	0.0	0.0	0.0	0.0
40	0.26611795494355744	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	25	3.418348E-5	46.0	14
ATCGTTC	20	6.312923E-4	46.0	41
CGAATAT	25	3.418348E-5	46.0	32
CGCGTAC	25	3.418348E-5	46.0	38
TCGCGTA	25	3.418348E-5	46.0	37
ACGACCG	30	1.8623123E-6	46.0	31
TTGGGAT	47045	0.0	44.15198	5
GTTTGGG	261700	0.0	44.056824	3
TTTGGGA	125640	0.0	44.04489	4
GGTTTGG	267955	0.0	43.99403	2
TTGGGAC	21075	0.0	43.91554	5
GGGTTTG	270300	0.0	43.80381	1
TTTGGGG	104470	0.0	43.404327	4
TGGGATC	13480	0.0	43.372406	6
TTTGGGC	23430	0.0	43.349552	4
CGATTTA	85	0.0	43.294117	29
TTGGGAG	37110	0.0	42.96308	5
TTGGGGT	28470	0.0	42.905865	5
CGCGAAA	145	0.0	42.827583	12
TTGGGGC	19690	0.0	42.729305	5
>>END_MODULE