FastQCFastQC Report
Fri 17 Jun 2016
SRR1527061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507342
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC59240.23626613361878834No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT51520.20547655644902052No Hit
GGGTTTGGGAGCTGAACCCACCTCACCCACCCTCTCCTTCGCAGGCAGGGAC42860.17093798931298562No Hit
GGGTTTGGGAGCTTCTTTGGGTCTGTGGATTGTACCATAGTTATCCTGCACT38370.15303057979326315No Hit
GGGTTTGGGGACTGGAGTTCAGATTTCTTCCAACGAAAGTTTTCTGTCTCTA38170.15223292235363187No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32380.12914073947630597No Hit
GGGTTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGC27480.10959813220533934No Hit
GGGTTTGGGATCTTGACTTGCAAAGTACGGTGTAGAGACCTACTGTGTGCTT26190.10445324171971754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA351.0202166E-746.00000413
CGCGAAA1550.046.012
ACCCGAT206.312888E-446.010
ATACGGC206.312888E-446.037
GCGTATC206.312888E-446.034
ATCGCGA206.312888E-446.016
TTGGGAT454950.044.0384645
GTTTGGG2541950.044.0311163
GGTTTGG2603250.043.9838262
TTTGGGA1213850.043.949834
GGGTTTG2623550.043.8311041
TTGGGAC204200.043.758575
TTTGGGG1018250.043.526644
TGGGATC131000.043.366416
TTTGGGC227700.043.3030324
TTGGGGT282150.042.9105075
TTGGGAG365650.042.7039535
CTTCGCA5500.042.6545537
TTGGGGC195900.042.5952035
TGGGATT174100.042.551986