##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527061_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2507342
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76709359951694	31.0	31.0	33.0	31.0	33.0
2	32.57991450707562	34.0	31.0	34.0	31.0	34.0
3	33.05124550220911	34.0	31.0	34.0	31.0	34.0
4	33.200929909043126	35.0	33.0	35.0	30.0	35.0
5	34.897374191474476	35.0	35.0	35.0	33.0	37.0
6	35.91746598589263	37.0	35.0	37.0	35.0	37.0
7	36.12802840617674	37.0	35.0	37.0	35.0	37.0
8	36.577791940628764	37.0	37.0	37.0	35.0	37.0
9	38.65125100604545	39.0	39.0	39.0	39.0	39.0
10	38.05652320265843	39.0	38.0	39.0	37.0	39.0
11	37.77727848853487	39.0	38.0	39.0	35.0	39.0
12	37.59123087317167	39.0	37.0	39.0	35.0	39.0
13	37.461370247856095	39.0	37.0	39.0	35.0	39.0
14	38.328035425562206	40.0	38.0	40.0	35.0	40.0
15	38.36991363762901	40.0	38.0	40.0	35.0	40.0
16	38.396661085723444	40.0	38.0	40.0	35.0	40.0
17	38.4147140677259	40.0	38.0	40.0	35.0	40.0
18	38.419316551152576	40.0	38.0	40.0	35.0	40.0
19	38.39353745918985	40.0	38.0	40.0	35.0	40.0
20	38.35083127870071	40.0	38.0	40.0	35.0	40.0
21	34.09439039429005	40.0	35.0	40.0	10.0	40.0
22	34.886498132285105	40.0	35.0	40.0	17.0	40.0
23	36.963369975057255	40.0	35.0	40.0	31.0	40.0
24	37.73508400529325	40.0	37.0	40.0	34.0	40.0
25	37.97320549011663	40.0	38.0	40.0	34.0	40.0
26	38.04558771798981	40.0	38.0	40.0	34.0	40.0
27	37.99689751138855	40.0	38.0	40.0	34.0	40.0
28	37.975765172840404	40.0	38.0	40.0	34.0	40.0
29	37.96922278652055	40.0	38.0	40.0	34.0	40.0
30	37.874410431444936	40.0	37.0	40.0	34.0	40.0
31	37.74331782421385	40.0	37.0	40.0	34.0	40.0
32	37.73310382069937	40.0	37.0	40.0	34.0	40.0
33	37.41156730912656	40.0	37.0	40.0	33.0	40.0
34	37.363552718376674	40.0	36.0	40.0	33.0	40.0
35	37.36125187549205	40.0	36.0	40.0	33.0	40.0
36	37.267788359146856	40.0	36.0	40.0	33.0	40.0
37	37.236469137437176	39.0	36.0	40.0	33.0	40.0
38	37.186193586674655	39.0	36.0	40.0	33.0	40.0
39	37.10783849989351	39.0	35.0	40.0	33.0	40.0
40	32.16508836847945	38.0	33.0	40.0	9.0	40.0
41	33.168324464712036	38.0	32.0	40.0	15.0	40.0
42	35.06896227160076	38.0	33.0	40.0	26.0	40.0
43	36.001715761152646	38.0	35.0	40.0	30.0	40.0
44	36.67164152317474	39.0	35.0	40.0	32.0	40.0
45	36.88875909229774	39.0	35.0	40.0	33.0	40.0
46	36.93043549703231	39.0	35.0	40.0	33.0	40.0
47	36.930826349177735	39.0	35.0	40.0	33.0	40.0
48	36.82570347403745	39.0	35.0	40.0	32.0	40.0
49	36.73893389892564	39.0	35.0	40.0	32.0	40.0
50	36.629381632023076	39.0	35.0	40.0	32.0	40.0
51	36.46255317383907	39.0	35.0	40.0	32.0	40.0
52	36.09897891871153	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	1.0
15	10.0
16	33.0
17	87.0
18	164.0
19	315.0
20	542.0
21	1091.0
22	1716.0
23	2999.0
24	4540.0
25	6943.0
26	9900.0
27	14635.0
28	20684.0
29	27800.0
30	37494.0
31	51037.0
32	69982.0
33	89443.0
34	163880.0
35	227143.0
36	336333.0
37	314092.0
38	862440.0
39	264036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.71511664543569	0.11227028462810419	0.14361822200561392	0.02899484793059742
8	99.52515452618749	0.2510227962519672	0.10923918635750526	0.11458349120303493
9	97.61001889650474	1.0704164011132107	0.5383788888791398	0.7811858135029046
10	39.29320371931711	47.52231646101728	4.250836144411093	8.933643675254514
11	28.19419927556751	22.418561169557243	32.38732490422128	16.99991465065396
12	27.136545393488404	18.29459244091951	28.766997082966743	25.801865082625348
13	24.186369470140093	18.511276084395348	32.44324069073944	24.859113754725122
14	25.761344084692077	21.83375861769156	30.072443248667312	22.332454048949046
15	26.219518518016287	23.103031018504854	29.58012907692688	21.09732138655197
16	28.766358957015036	25.639262613556507	26.161369290667167	19.433009138761285
17	27.544985885451606	23.15061128477886	25.76190244489982	23.54250038486971
18	25.33116742749892	23.24712783497425	28.149929287667973	23.271775449858858
19	25.72748352637973	24.105287591401574	28.034627904769273	22.132600977449428
20	25.78607146532065	25.48100737753366	26.929752702263993	21.803168454881703
21	30.73234524847428	22.779979755454182	27.29994551999687	19.187729476074665
22	25.289769006382055	23.197314127869273	29.505308809089463	22.007608056659205
23	23.48877815631055	25.774385783830052	29.010242719182305	21.7265933406771
24	24.364566142153723	24.6326588076138	28.367211174223538	22.635563876008938
25	24.25357210942903	26.85684681228169	26.931667080119105	21.957913998170174
26	24.522382666584775	26.58073768955332	27.019728461454402	21.877151182407506
27	22.21424121639569	25.202505282486392	27.6397475892798	24.943505911838113
28	23.75703035325855	25.960239967264137	27.112017427219744	23.17071225225757
29	25.45273042129873	25.78662982552839	27.000026322695508	21.760613430477374
30	24.46028503490948	24.927632528789452	27.64138278703105	22.970699649270024
31	25.318684088568695	25.833930911698523	26.416141076885403	22.431243922847383
32	26.24552215054827	25.20880677625948	27.311511552871526	21.234159520320723
33	25.636949406981575	26.146054267826248	27.18404589401845	21.03295043117373
34	23.613212716893027	27.06431751232979	28.603078479122512	20.719391291654667
35	23.742114159137444	28.931832992866553	27.399572934206823	19.926479913789183
36	23.309265349521525	27.461550917266177	27.32255910841042	21.906624624801882
37	24.394956890603677	28.186541764147055	25.817140222594286	21.601361122654986
38	23.413718591241242	28.043960496812957	26.716498985778564	21.825821926167233
39	24.975093146447513	26.58420749941572	26.08371733891906	22.356982015217707
40	24.599316726637213	31.405727659011017	23.530495640403267	20.464459973948507
41	22.47495555053918	28.59741511130113	26.473333115306968	22.454296222852726
42	22.110386217755696	27.742725164736203	26.804480601369896	23.342408016138204
43	22.949840907223663	27.72784885348708	26.332347162852138	22.98996307643712
44	22.99965461432864	27.586543838056393	26.63250565738539	22.781295890229575
45	23.176973863158675	27.40515653628424	25.73366537153687	23.684204229020214
46	22.32595314081605	28.606229226009056	25.756318842822402	23.31149879035249
47	22.45593142060397	28.45359747493561	27.123344162862505	21.967126941597915
48	22.290377619008495	28.15714808749664	26.827572784247224	22.72490150924764
49	23.5421015561499	28.04858690996282	25.93224219113308	22.4770693427542
50	22.354668808642778	28.870971730222685	26.09815493857639	22.67620452255815
51	21.242973635028648	29.156373562122756	26.38244005006098	23.21821275278761
52	21.53208457402301	28.13046644614097	27.500436717448196	22.83701226238782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	38.0
6	76.0
7	70.5
8	65.0
9	118.5
10	172.0
11	1796.5
12	3421.0
13	8814.5
14	17408.5
15	20609.0
16	18260.0
17	15911.0
18	15706.0
19	15501.0
20	14601.5
21	13702.0
22	14441.0
23	15180.0
24	16121.5
25	17063.0
26	21318.0
27	25573.0
28	28216.5
29	30860.0
30	34996.5
31	39133.0
32	44500.0
33	49867.0
34	54871.0
35	59875.0
36	61852.0
37	63829.0
38	71065.0
39	90894.0
40	103487.0
41	102823.5
42	102160.0
43	108970.5
44	115781.0
45	115289.5
46	114798.0
47	125491.5
48	136185.0
49	140390.0
50	144595.0
51	146277.5
52	147960.0
53	156520.5
54	165081.0
55	166440.0
56	167799.0
57	170788.5
58	173778.0
59	168703.5
60	163629.0
61	152108.5
62	140588.0
63	132759.0
64	108205.0
65	91480.0
66	74680.0
67	57880.0
68	48020.5
69	38161.0
70	29243.5
71	20326.0
72	18347.5
73	16369.0
74	13852.0
75	11335.0
76	7226.0
77	3117.0
78	2390.0
79	1663.0
80	1825.0
81	1987.0
82	1416.5
83	846.0
84	441.5
85	37.0
86	23.5
87	10.0
88	7.5
89	4.0
90	3.0
91	3.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2507342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.159980414539238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.05009500545825	5.716074032811521
2	9.72245468108251	1.5867007955775725
3	4.097260630368034	1.0030069949119753
4	2.119102105606602	0.6916732671263494
5	1.1757976202756613	0.47972427764556197
6	0.6963089208366499	0.34091202938976095
7	0.5017179903328036	0.28658062823163655
8	0.33292593852794655	0.2173335310304111
9	0.2715649951879018	0.1994368537806949
>10	4.104423534720918	9.37030230942823
>50	3.3873971214477017	20.14069030431632
>100	3.406375747085681	50.000486763685394
>500	0.10697043541413756	5.853998928038725
>1k	0.026455053919625417	3.59329531289379
>5k	0.0011502197356358878	0.519783971132072
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5924	0.23626613361878834	No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5152	0.20547655644902052	No Hit
GGGTTTGGGAGCTGAACCCACCTCACCCACCCTCTCCTTCGCAGGCAGGGAC	4286	0.17093798931298562	No Hit
GGGTTTGGGAGCTTCTTTGGGTCTGTGGATTGTACCATAGTTATCCTGCACT	3837	0.15303057979326315	No Hit
GGGTTTGGGGACTGGAGTTCAGATTTCTTCCAACGAAAGTTTTCTGTCTCTA	3817	0.15223292235363187	No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3238	0.12914073947630597	No Hit
GGGTTTGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGC	2748	0.10959813220533934	No Hit
GGGTTTGGGATCTTGACTTGCAAAGTACGGTGTAGAGACCTACTGTGTGCTT	2619	0.10445324171971754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.988287198156454E-5	0.0	0.0	0.0	0.0
8	7.976574396312908E-5	0.0	0.0	0.0	0.0
9	5.982430797234681E-4	0.0	0.0	0.0	0.0
10	0.012323807442303443	0.0	0.0	0.0	0.0
11	0.019462841527003494	0.0	0.0	0.0	0.0
12	0.02520597509234879	0.0	0.0	0.0	0.0
13	0.029632973882302453	0.0	0.0	0.0	0.0
14	0.03900544879797012	0.0	0.0	0.0	0.0
15	0.05037206731271601	0.0	0.0	0.0	0.0
16	0.062376811779166944	0.0	0.0	0.0	0.0
17	0.06915690001603292	0.0	0.0	0.0	0.0
18	0.07545839378912012	0.0	0.0	0.0	0.0
19	0.08507016593667717	0.0	0.0	0.0	0.0
20	0.0931265060769532	0.0	0.0	0.0	0.0
21	0.0998667114418376	0.0	0.0	0.0	0.0
22	0.1083218803019293	0.0	0.0	0.0	0.0
23	0.11621868895427907	0.0	0.0	0.0	0.0
24	0.12535186663805736	0.0	0.0	0.0	0.0
25	0.13253078359473897	0.0	0.0	0.0	0.0
26	0.1392311060876418	0.0	0.0	0.0	0.0
27	0.14692850038008376	0.0	0.0	0.0	0.0
28	0.15789629017501403	0.0	0.0	0.0	0.0
29	0.16451684692395374	0.0	0.0	0.0	0.0
30	0.17201482685648786	0.0	0.0	0.0	0.0
31	0.17967233827694826	0.0	0.0	0.0	0.0
32	0.18764891267326117	0.0	0.0	0.0	0.0
33	0.1941099379342746	0.0	0.0	0.0	0.0
34	0.20240557530644004	0.0	0.0	0.0	0.0
35	0.21815930973915804	0.0	0.0	0.0	0.0
36	0.2270930730630285	0.0	0.0	0.0	0.0
37	0.23415234140376542	0.0	0.0	0.0	0.0
38	0.2428468074957465	0.0	0.0	0.0	0.0
39	0.25022513881233593	0.0	0.0	0.0	0.0
40	0.25939819936809577	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGTA	35	1.0202166E-7	46.000004	13
CGCGAAA	155	0.0	46.0	12
ACCCGAT	20	6.312888E-4	46.0	10
ATACGGC	20	6.312888E-4	46.0	37
GCGTATC	20	6.312888E-4	46.0	34
ATCGCGA	20	6.312888E-4	46.0	16
TTGGGAT	45495	0.0	44.038464	5
GTTTGGG	254195	0.0	44.031116	3
GGTTTGG	260325	0.0	43.983826	2
TTTGGGA	121385	0.0	43.94983	4
GGGTTTG	262355	0.0	43.831104	1
TTGGGAC	20420	0.0	43.75857	5
TTTGGGG	101825	0.0	43.52664	4
TGGGATC	13100	0.0	43.36641	6
TTTGGGC	22770	0.0	43.303032	4
TTGGGGT	28215	0.0	42.910507	5
TTGGGAG	36565	0.0	42.703953	5
CTTCGCA	550	0.0	42.65455	37
TTGGGGC	19590	0.0	42.595203	5
TGGGATT	17410	0.0	42.55198	6
>>END_MODULE