FastQCFastQC Report
Fri 17 Jun 2016
SRR1527060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744093
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC53150.714292433875873No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC52570.7064977092917148No Hit
GAGATCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC30840.41446432099213404No Hit
GAGATCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA28560.38382298986820196No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT28460.3824790718364506No Hit
GAGATCGGGATTTCAGGATAATTATCAGAACCAGATATTTCATGATCAAG27190.36541131283320766No Hit
GAGATCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT22000.2956619669853096No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGA21970.2952587915757842No Hit
GAGATCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA18420.24754970144860924No Hit
GAGATCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC17610.23666396539142287No Hit
GAGATCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC16490.22161208343580707No Hit
GAGATCGGGAGTTTAAATTACATGTGTGAGCACCATTGTGGCTAGTAAGC15720.21126391459132124No Hit
GAGATCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15410.2070977686928919No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC14220.19110514411505014No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC13540.18196650149914056No Hit
GAGATCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGA13250.17806913920706147No Hit
GAGATCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA12880.1730966424895813No Hit
GAGATCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG12680.17040880642607847No Hit
GAGATCGGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTG12620.1696024556070276No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT12050.1619421228260446No Hit
GAGATCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAG11650.15656645069903896No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA11590.15576009987998812No Hit
GAGATCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGA10950.14715902447677912No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC10470.14070821792437235No Hit
GAGATCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG10140.13627328841959271No Hit
GAGATCGGGAACGTTACTGTGGATAATAGAGTTAAATGTGAACGGAGGCA9970.13398862776561532No Hit
GAGATCGGCGTCTGTGCTTACTTGGTTCATGCTTCTTGTGTGACACTTCT9720.1306288326862368No Hit
GAGATCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG9630.12941930645766053No Hit
GAGATCGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATG9590.12888173924495996No Hit
GAGATCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT9230.1240436343306549No Hit
GAGATCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT9130.12269971629890349No Hit
GAGATCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA9090.12216214908620295No Hit
GAGATCGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA8890.11947431302270012No Hit
GAGATCGGGATTGTTTGTAGTCATTTTAAATAGCATGAGCCATGTCCCTG8850.11893674580999956No Hit
GAGATCGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTG8660.11638330154967189No Hit
GAGATCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA8590.1154425589274459No Hit
GAGATCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT8430.11329229007664364No Hit
GAGATCGGGTCGCTTTTCTCTTGAAGGTGGTAGCCATTAGCTTAAGCTGT8240.11073884581631598No Hit
GAGATCGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAG8060.10831979335916343No Hit
GAGATCGGGGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTG8030.10791661794963801No Hit
GAGATCGGAAAATGCAGCTCCTCCACCCCAGCCCACTGTTCGCTCTTGTA8020.10778222614646288No Hit
GAGATCGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGC8000.1075134425401126No Hit
GAGATCGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTC7980.10724465893376231No Hit
GAGATCGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTC7940.10670709172106174No Hit
GAGATCGGGGTCACTGGTATCTTGCTAGTGATCATTTCTTATATCGTCCC7870.10576634909883577No Hit
GAGATCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAA7840.10536317368931034No Hit
GAGATCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC7740.10401925565755893No Hit
GAGATCGGACTTGAAGCTGAGCCTCTATGTGGGACTTTTAAAAAAAAAAA7600.10213777041310695No Hit
GAGATCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG7550.10146581139723126No Hit
GAGATCGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG7510.10092824418453068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGT302.5265072E-644.00000437
AGCGTAT302.5265072E-644.00000414
CGTATTG302.5265072E-644.00000416
GGTGTAC302.5265072E-644.00000441
CATGCGA302.5265072E-644.00000429
CCCTAGT302.5265072E-644.00000438
TAGCGTA302.5265072E-644.00000413
ACATTCG302.5265072E-644.00000437
GCACGTA302.5265072E-644.00000430
TAAACGA302.5265072E-644.00000440
GTTAGTC302.5265072E-644.00000410
ATTCGTC302.5265072E-644.00000439
TTACTCG302.5265072E-644.00000443
CCCACGT302.5265072E-644.00000411
TAGTTAT302.5265072E-644.00000429
CTAACTA302.5265072E-644.00000441
CTAAATG302.5265072E-644.00000410
GTTCGCC302.5265072E-644.00000427
CGCTATC302.5265072E-644.00000443
ACTACAC302.5265072E-644.00000426
Produced by FastQC (version 0.11.5)