##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744093
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46165331484102	33.0	31.0	34.0	31.0	34.0
2	32.31161158618614	34.0	31.0	34.0	31.0	34.0
3	32.84299543202261	34.0	31.0	34.0	31.0	34.0
4	36.1904976931647	37.0	37.0	37.0	35.0	37.0
5	36.153913556504364	37.0	37.0	37.0	35.0	37.0
6	36.26068918804504	37.0	37.0	37.0	35.0	37.0
7	36.554898379638026	37.0	37.0	37.0	35.0	37.0
8	36.67244282636713	37.0	37.0	37.0	35.0	37.0
9	38.70291751165513	39.0	39.0	39.0	39.0	39.0
10	38.211817608820404	39.0	39.0	39.0	37.0	39.0
11	37.95018364639904	39.0	38.0	39.0	35.0	39.0
12	37.61911481494921	39.0	37.0	39.0	35.0	39.0
13	37.566796085973124	39.0	37.0	39.0	35.0	39.0
14	38.441692100315414	40.0	38.0	40.0	35.0	40.0
15	38.42366209600144	40.0	38.0	40.0	35.0	40.0
16	38.281292795389824	40.0	38.0	40.0	35.0	40.0
17	38.380064051133395	40.0	38.0	40.0	35.0	40.0
18	38.31803685829594	40.0	38.0	40.0	35.0	40.0
19	38.23858308034077	40.0	38.0	40.0	35.0	40.0
20	37.99893292908279	40.0	38.0	40.0	34.0	40.0
21	38.19715411917596	40.0	38.0	40.0	34.0	40.0
22	37.93833700894915	40.0	38.0	40.0	34.0	40.0
23	37.929003498218634	40.0	38.0	40.0	34.0	40.0
24	37.95178156493879	40.0	38.0	40.0	34.0	40.0
25	37.86166782915576	40.0	38.0	40.0	34.0	40.0
26	37.70613888317724	40.0	37.0	40.0	33.0	40.0
27	37.528615374691064	40.0	37.0	40.0	33.0	40.0
28	37.33669984800287	40.0	37.0	40.0	33.0	40.0
29	37.58027692774962	40.0	37.0	40.0	33.0	40.0
30	37.609001831760274	40.0	37.0	40.0	33.0	40.0
31	37.595838154639274	40.0	37.0	40.0	33.0	40.0
32	36.777932328351426	40.0	36.0	40.0	31.0	40.0
33	36.96074281037451	40.0	36.0	40.0	31.0	40.0
34	36.89331978663957	39.0	35.0	40.0	31.0	40.0
35	36.93354728508399	39.0	35.0	40.0	32.0	40.0
36	36.543112218499566	39.0	35.0	40.0	31.0	40.0
37	36.459559490547555	39.0	35.0	40.0	31.0	40.0
38	36.19256598301556	39.0	35.0	40.0	30.0	40.0
39	36.07000872202803	38.0	35.0	40.0	30.0	40.0
40	35.8356428564709	38.0	35.0	40.0	30.0	40.0
41	35.736745272432344	38.0	35.0	40.0	30.0	40.0
42	35.60590141286103	38.0	35.0	40.0	29.0	40.0
43	35.43730420794175	37.0	35.0	40.0	29.0	40.0
44	35.253386337460505	37.0	34.0	40.0	29.0	40.0
45	35.07645684074437	37.0	34.0	40.0	28.0	40.0
46	34.717156323201536	36.0	34.0	40.0	27.0	40.0
47	34.634330654904694	36.0	34.0	40.0	27.0	40.0
48	34.46463681287151	35.0	34.0	39.0	27.0	40.0
49	34.097004003531815	35.0	33.0	39.0	26.0	40.0
50	33.64669201296075	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	3.0
16	28.0
17	45.0
18	96.0
19	237.0
20	462.0
21	632.0
22	913.0
23	1403.0
24	2075.0
25	2923.0
26	3899.0
27	5372.0
28	7651.0
29	9711.0
30	12428.0
31	15446.0
32	19324.0
33	25816.0
34	39293.0
35	52973.0
36	87506.0
37	143974.0
38	220020.0
39	91860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.65541941665894	0.262601583404225	0.07069008847012404	0.011288911466711822
8	99.24901860385731	0.5842011684023368	0.0805006901019093	0.08627953763844036
9	94.32893468961541	3.844680705234426	0.6370171470501671	1.1893674580999956
10	48.50227054951464	32.64108115517818	6.030966559287616	12.825681736019556
11	30.57279130431277	20.79269661184825	24.630926510530273	24.003585573308715
12	22.23606457794926	16.518499703666073	33.0415687286401	28.203866989744565
13	22.60483568586185	16.821418828022843	32.9167187434904	27.657026742624915
14	21.798081691401478	18.647534649566655	32.16506538833184	27.389318270700038
15	23.906151516006734	18.181195092548915	29.87072852452583	28.041924866918517
16	28.498319430501297	18.353485384219447	25.18018581010707	27.968009375172187
17	30.759192735316688	17.50842972585416	22.935977088885394	28.79640044994376
18	28.892087413804457	17.59282777824815	24.34077460747514	29.174310200472252
19	29.243387587304277	18.141011943399548	23.784526934133233	28.831073535162943
20	28.904585851499746	18.962549036209182	24.054923242121617	28.077941870169454
21	32.31907839477054	16.844265434562615	23.483892470430444	27.352763700236395
22	32.36544356686597	17.036445713103067	21.97615083060854	28.621959889422428
23	29.141115425087992	19.458454789925455	23.631185886710398	27.76924389827616
24	30.206036073447805	19.464771204674687	22.68829299563361	27.640899726243894
25	28.212199281541423	19.61394610619909	23.452847963896986	28.7210066483625
26	29.674247708283772	18.104591764739087	22.22222222222222	29.99893830475492
27	30.300782294686286	17.723725394540736	23.617881098196058	28.357611212576924
28	30.299572768457704	19.42122826044594	22.851444644688232	27.42775432640812
29	31.140999848137263	18.419606151381615	20.882470336369245	29.556923664111878
30	29.532867531343527	21.154479345995732	20.63263597426666	28.680017148394082
31	32.20175435059865	20.756814000400485	20.310498822055845	26.73093282694502
32	32.17689186701125	20.09963808287405	21.129751254211506	26.5937187959032
33	31.83607425415909	19.5295480538051	22.781157731627633	25.853219960408175
34	29.725047809883982	21.976554006018066	23.43940878357947	24.85898940051848
35	32.94010291724287	21.201516477107027	21.76959062912835	24.088789976521753
36	32.76794701737551	20.65911115949216	20.967943523188634	25.604998299943688
37	30.94142802042218	21.887855415922473	21.71475877343289	25.45595779022246
38	32.83433656814403	20.952622857626668	20.244781230303204	25.968259343926096
39	30.402382497886688	21.790421358620495	21.766768401261672	26.04042774223115
40	29.70999592792836	21.717581001299568	22.71073642676386	25.86168664400821
41	28.95068223998882	20.903704241270916	21.467209071984282	28.678404446755984
42	28.326566706043465	21.055566978858824	23.502169755662266	27.11569655943545
43	27.381792329722227	20.906795252743944	25.58349561143567	26.127916806098163
44	29.384230197031826	19.370965726058436	24.08247356177252	27.162330515137224
45	29.157645616878536	20.10904550909631	23.276122742721675	27.45718613130348
46	29.025404082554196	20.527272800577347	23.549744453986264	26.897578662882193
47	29.368909531469857	20.48346107274225	23.368046736093472	26.779582659694416
48	28.695740989365575	21.658179824296155	23.72714163417745	25.91893755216082
49	28.0463597964233	21.746475238982224	24.741799748149763	25.46536521644472
50	26.67112847453208	22.77013760376727	23.445053239312827	27.11368068238782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	19.5
6	39.0
7	26.5
8	14.0
9	29.5
10	45.0
11	495.5
12	946.0
13	869.5
14	793.0
15	938.5
16	1084.0
17	973.5
18	863.0
19	822.5
20	782.0
21	1065.5
22	1349.0
23	1060.5
24	772.0
25	1322.5
26	1873.0
27	3079.0
28	4285.0
29	5349.0
30	6413.0
31	4724.5
32	3036.0
33	3989.0
34	4942.0
35	7183.0
36	9424.0
37	8938.0
38	8452.0
39	9233.5
40	10015.0
41	11274.0
42	12533.0
43	14446.0
44	16359.0
45	17965.5
46	19572.0
47	20492.5
48	21413.0
49	22779.0
50	24145.0
51	25438.5
52	26732.0
53	30850.5
54	34969.0
55	44053.5
56	53138.0
57	64861.5
58	76585.0
59	74537.5
60	72490.0
61	73859.0
62	75228.0
63	72307.5
64	69387.0
65	62704.0
66	56021.0
67	48328.5
68	40636.0
69	32448.5
70	24261.0
71	23044.0
72	21827.0
73	19801.5
74	17776.0
75	17475.5
76	17175.0
77	11211.0
78	5247.0
79	3688.0
80	2129.0
81	1531.5
82	934.0
83	598.5
84	263.0
85	180.0
86	97.0
87	69.5
88	42.0
89	24.5
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	744093.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.324256510946885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.16097219420149	8.171693592064432
2	11.006016875734307	2.4926991652925103
3	4.260470194510046	1.4473997201962656
4	2.0720838327617104	0.938592353375183
5	1.2164295123601105	0.6887579912725963
6	0.6871343294209795	0.466877124230439
7	0.4272337799508681	0.3386673440013547
8	0.3334797004616498	0.3021127735377164
9	0.288382801466836	0.2939148735440328
>10	3.0381068796506177	9.332838771497649
>50	2.2678993152391915	18.89347164937716
>100	2.1195542527562514	42.79021573916164
>500	0.09256731898935476	7.1980249780605385
>1k	0.027295491496861023	5.223943781220896
>5k	0.002373520999727045	1.420790143167588
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5315	0.714292433875873	No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	5257	0.7064977092917148	No Hit
GAGATCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3084	0.41446432099213404	No Hit
GAGATCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2856	0.38382298986820196	No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2846	0.3824790718364506	No Hit
GAGATCGGGATTTCAGGATAATTATCAGAACCAGATATTTCATGATCAAG	2719	0.36541131283320766	No Hit
GAGATCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	2200	0.2956619669853096	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGA	2197	0.2952587915757842	No Hit
GAGATCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1842	0.24754970144860924	No Hit
GAGATCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC	1761	0.23666396539142287	No Hit
GAGATCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	1649	0.22161208343580707	No Hit
GAGATCGGGAGTTTAAATTACATGTGTGAGCACCATTGTGGCTAGTAAGC	1572	0.21126391459132124	No Hit
GAGATCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1541	0.2070977686928919	No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1422	0.19110514411505014	No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1354	0.18196650149914056	No Hit
GAGATCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGA	1325	0.17806913920706147	No Hit
GAGATCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1288	0.1730966424895813	No Hit
GAGATCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1268	0.17040880642607847	No Hit
GAGATCGGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTG	1262	0.1696024556070276	No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	1205	0.1619421228260446	No Hit
GAGATCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAG	1165	0.15656645069903896	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA	1159	0.15576009987998812	No Hit
GAGATCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGA	1095	0.14715902447677912	No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC	1047	0.14070821792437235	No Hit
GAGATCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1014	0.13627328841959271	No Hit
GAGATCGGGAACGTTACTGTGGATAATAGAGTTAAATGTGAACGGAGGCA	997	0.13398862776561532	No Hit
GAGATCGGCGTCTGTGCTTACTTGGTTCATGCTTCTTGTGTGACACTTCT	972	0.1306288326862368	No Hit
GAGATCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	963	0.12941930645766053	No Hit
GAGATCGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATG	959	0.12888173924495996	No Hit
GAGATCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	923	0.1240436343306549	No Hit
GAGATCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	913	0.12269971629890349	No Hit
GAGATCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	909	0.12216214908620295	No Hit
GAGATCGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA	889	0.11947431302270012	No Hit
GAGATCGGGATTGTTTGTAGTCATTTTAAATAGCATGAGCCATGTCCCTG	885	0.11893674580999956	No Hit
GAGATCGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTG	866	0.11638330154967189	No Hit
GAGATCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	859	0.1154425589274459	No Hit
GAGATCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	843	0.11329229007664364	No Hit
GAGATCGGGTCGCTTTTCTCTTGAAGGTGGTAGCCATTAGCTTAAGCTGT	824	0.11073884581631598	No Hit
GAGATCGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAG	806	0.10831979335916343	No Hit
GAGATCGGGGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTG	803	0.10791661794963801	No Hit
GAGATCGGAAAATGCAGCTCCTCCACCCCAGCCCACTGTTCGCTCTTGTA	802	0.10778222614646288	No Hit
GAGATCGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGC	800	0.1075134425401126	No Hit
GAGATCGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTC	798	0.10724465893376231	No Hit
GAGATCGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTC	794	0.10670709172106174	No Hit
GAGATCGGGGTCACTGGTATCTTGCTAGTGATCATTTCTTATATCGTCCC	787	0.10576634909883577	No Hit
GAGATCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAA	784	0.10536317368931034	No Hit
GAGATCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	774	0.10401925565755893	No Hit
GAGATCGGACTTGAAGCTGAGCCTCTATGTGGGACTTTTAAAAAAAAAAA	760	0.10213777041310695	No Hit
GAGATCGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTG	755	0.10146581139723126	No Hit
GAGATCGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG	751	0.10092824418453068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.6805600912789127	0.0	0.0	0.0	0.0
3	0.6805600912789127	0.0	0.0	0.0	0.0
4	0.6805600912789127	0.0	0.0	0.0	0.0
5	0.6805600912789127	0.0	0.0	0.0	0.0
6	0.6805600912789127	0.0	0.0	0.0	0.0
7	0.681769617507489	0.0	0.0	0.0	0.0
8	0.682710360129715	0.0	0.0	0.0	0.0
9	0.6837854945551161	0.0	0.0	0.0	0.0
10	0.691311435532924	0.0	0.0	0.0	0.0
11	0.6988373765107319	0.0	0.0	0.0	0.0
12	0.7036754814250369	0.0	0.0	0.0	0.0
13	0.7114702060091951	0.0	0.0	0.0	0.0
14	0.7344512043521442	0.0	0.0	0.0	0.0
15	0.7449337649998051	0.0	0.0	0.0	0.0
16	0.7575665944982684	0.0	0.0	0.0	0.0
17	0.7671084125237033	0.0	0.0	0.0	0.0
18	0.7763814469427881	0.0	0.0	0.0	0.0
19	0.7835042125110705	0.0	0.0	0.0	0.0
20	0.7951962993873077	0.0	0.0	0.0	0.0
21	0.8123984501937258	0.0	0.0	0.0	0.0
22	0.8253000632985393	0.0	0.0	0.0	0.0
23	0.8420990386954319	0.0	0.0	0.0	0.0
24	0.8545974763907199	0.0	0.0	0.0	0.0
25	0.8722028026066634	0.0	0.0	0.0	0.0
26	0.8879266435781549	0.0	0.0	0.0	0.0
27	0.9029785255337707	0.0	0.0	0.0	0.0
28	0.9238092550259175	0.0	0.0	0.0	0.0
29	0.9355013419021547	0.0	0.0	0.0	0.0
30	0.9458495107466406	0.0	0.0	0.0	0.0
31	0.9604982172927309	0.0	0.0	0.0	0.0
32	0.9735342222007195	0.0	0.0	0.0	0.0
33	0.9838823910452054	0.0	0.0	0.0	0.0
34	0.9943649516928663	0.0	0.0	0.0	0.0
35	1.0196306106897928	0.0	0.0	0.0	0.0
36	1.030247563140629	0.0	0.0	0.0	0.0
37	1.0401925565755894	0.0	0.0	0.0	0.0
38	1.0500031582073746	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGT	30	2.5265072E-6	44.000004	37
AGCGTAT	30	2.5265072E-6	44.000004	14
CGTATTG	30	2.5265072E-6	44.000004	16
GGTGTAC	30	2.5265072E-6	44.000004	41
CATGCGA	30	2.5265072E-6	44.000004	29
CCCTAGT	30	2.5265072E-6	44.000004	38
TAGCGTA	30	2.5265072E-6	44.000004	13
ACATTCG	30	2.5265072E-6	44.000004	37
GCACGTA	30	2.5265072E-6	44.000004	30
TAAACGA	30	2.5265072E-6	44.000004	40
GTTAGTC	30	2.5265072E-6	44.000004	10
ATTCGTC	30	2.5265072E-6	44.000004	39
TTACTCG	30	2.5265072E-6	44.000004	43
CCCACGT	30	2.5265072E-6	44.000004	11
TAGTTAT	30	2.5265072E-6	44.000004	29
CTAACTA	30	2.5265072E-6	44.000004	41
CTAAATG	30	2.5265072E-6	44.000004	10
GTTCGCC	30	2.5265072E-6	44.000004	27
CGCTATC	30	2.5265072E-6	44.000004	43
ACTACAC	30	2.5265072E-6	44.000004	26
>>END_MODULE