SRR1527059_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800345
Sequences flagged as poor quality	0
Sequence length	52
%GC	58

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5137	0.6418482029624725	No Hit
GAGATCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4953	0.6188581174368554	No Hit
GAGATCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2810	0.35109858873360866	No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2770	0.34610074405412666	No Hit
GAGATCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2735	0.34172762995957995	No Hit
GAGATCGGGATTTCAGGATAATTATCAGAACCAGATATTTCATGATCAAGAG	2682	0.3351054857592663	No Hit
GAGATCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	2166	0.2706332893939489	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG	2011	0.2512666412609562	No Hit
GAGATCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1791	0.22377849552380535	No Hit
GAGATCGGGAGTTTAAATTACATGTGTGAGCACCATTGTGGCTAGTAAGCCA	1717	0.2145324828667637	No Hit
GAGATCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT	1689	0.21103399159112632	No Hit
GAGATCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1584	0.19791464930748615	No Hit
GAGATCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1422	0.17767337835558414	No Hit
GAGATCGGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCT	1328	0.16592844335880153	No Hit
GAGATCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1302	0.16267984431713822	No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1253	0.1565574845847728	No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1202	0.1501852326184333	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG	1168	0.1459370646408736	No Hit
GAGATCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG	1155	0.14431276512004199	No Hit
GAGATCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT	1113	0.13906502820658592	No Hit
GAGATCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1100	0.13744072868575427	No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1072	0.1339422374101169	No Hit
GAGATCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG	1013	0.12657041650788098	No Hit
GAGATCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	975	0.12182246406237311	No Hit
GAGATCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	959	0.11982332619058032	No Hit
GAGATCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	945	0.11807408055276161	No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT	934	0.11669967326590408	No Hit
GAGATCGGAAAATGCAGCTCCTCCACCCCAGCCCACTGTTCGCTCTTGTAGT	891	0.11132699023546094	No Hit
GAGATCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	875	0.10932785236366815	No Hit
GAGATCGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	861	0.10757860672584948	No Hit
GAGATCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	852	0.10645409167296604	No Hit
GAGATCGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGT	849	0.1060792533220049	No Hit
GAGATCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG	832	0.10395516933322504	No Hit
GAGATCGGGTCGCTTTTCTCTTGAAGGTGGTAGCCATTAGCTTAAGCTGTAG	813	0.1015811931104711	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	6.310299E-4	46.000004	31
TTAGGTA	40	5.6079443E-9	46.000004	14
TTAGGGT	20	6.310299E-4	46.000004	14
ACACGCT	20	6.310299E-4	46.000004	45
ACACGAT	20	6.310299E-4	46.000004	28
CGTATCT	20	6.310299E-4	46.000004	33
GTCGACA	40	5.6079443E-9	46.000004	10
AAATAGG	40	5.6079443E-9	46.000004	24
CGACGTC	20	6.310299E-4	46.000004	19
GGTCGAA	20	6.310299E-4	46.000004	18
CATGCGA	40	5.6079443E-9	46.000004	29
CCCTAAT	20	6.310299E-4	46.000004	16
CGGTTAG	20	6.310299E-4	46.000004	14
TTATGCG	20	6.310299E-4	46.000004	14
TATATGA	80	0.0	46.000004	20
ATTCGGT	40	5.6079443E-9	46.000004	10
GTCTAAC	20	6.310299E-4	46.000004	20
GCGTTTA	20	6.310299E-4	46.000004	42
TAACGAA	20	6.310299E-4	46.000004	45
CCTAACA	20	6.310299E-4	46.000004	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content