##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800345
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37779957393374	33.0	31.0	34.0	31.0	34.0
2	32.193206679619415	33.0	31.0	34.0	31.0	34.0
3	32.79420874747765	34.0	31.0	34.0	31.0	34.0
4	36.13624999219087	37.0	35.0	37.0	35.0	37.0
5	36.125569598110815	37.0	37.0	37.0	35.0	37.0
6	36.24948366017155	37.0	37.0	37.0	35.0	37.0
7	36.55212064797056	37.0	37.0	37.0	35.0	37.0
8	36.635434718777525	37.0	37.0	37.0	35.0	37.0
9	38.66349261880814	39.0	39.0	39.0	38.0	39.0
10	38.10225465268103	39.0	39.0	39.0	37.0	39.0
11	37.840176423917185	39.0	38.0	39.0	35.0	39.0
12	37.57668505457022	39.0	37.0	39.0	35.0	39.0
13	37.57017411241402	39.0	37.0	39.0	35.0	39.0
14	38.45242988961011	40.0	38.0	40.0	35.0	40.0
15	38.33382603752132	40.0	38.0	40.0	35.0	40.0
16	33.2048479093391	40.0	35.0	40.0	3.0	40.0
17	34.95135972611811	40.0	35.0	40.0	17.0	40.0
18	36.99129750295185	40.0	35.0	40.0	31.0	40.0
19	37.82469560002249	40.0	37.0	40.0	34.0	40.0
20	38.0758647833122	40.0	38.0	40.0	34.0	40.0
21	38.298017729854	40.0	38.0	40.0	35.0	40.0
22	38.280783911937974	40.0	38.0	40.0	35.0	40.0
23	38.18235011151441	40.0	38.0	40.0	34.0	40.0
24	38.139923408030285	40.0	38.0	40.0	34.0	40.0
25	38.065671679088396	40.0	38.0	40.0	34.0	40.0
26	37.95039014425029	40.0	38.0	40.0	34.0	40.0
27	37.80480792658166	40.0	37.0	40.0	34.0	40.0
28	37.655703477875164	40.0	37.0	40.0	33.0	40.0
29	37.431785042700334	40.0	37.0	40.0	33.0	40.0
30	37.39508337029656	40.0	37.0	40.0	33.0	40.0
31	37.34963546970369	40.0	36.0	40.0	33.0	40.0
32	37.15209440928599	39.0	36.0	40.0	32.0	40.0
33	36.803237353891134	39.0	35.0	40.0	31.0	40.0
34	36.663645053070866	39.0	35.0	40.0	31.0	40.0
35	36.63535975110734	39.0	35.0	40.0	31.0	40.0
36	36.37447850614423	38.0	35.0	40.0	31.0	40.0
37	35.98331344607638	38.0	35.0	40.0	30.0	40.0
38	35.91594000087462	38.0	35.0	40.0	30.0	40.0
39	35.45115044137216	38.0	34.0	40.0	29.0	40.0
40	35.28711618114688	37.0	34.0	40.0	28.0	40.0
41	35.27002480180422	37.0	34.0	40.0	28.0	40.0
42	35.212684529796526	37.0	34.0	40.0	28.0	40.0
43	35.00704446207573	37.0	34.0	40.0	28.0	40.0
44	35.29291867881976	37.0	34.0	40.0	29.0	40.0
45	35.304126345513495	37.0	34.0	40.0	30.0	40.0
46	35.19853937989242	36.0	34.0	40.0	30.0	40.0
47	34.90439497966502	36.0	34.0	40.0	29.0	40.0
48	34.806336017592415	35.0	34.0	39.0	28.0	40.0
49	34.68341652662289	35.0	34.0	39.0	28.0	40.0
50	34.15257420237523	35.0	33.0	39.0	27.0	40.0
51	34.22526785323829	35.0	33.0	39.0	27.0	40.0
52	33.81918922464687	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	3.0
16	19.0
17	42.0
18	113.0
19	173.0
20	343.0
21	572.0
22	921.0
23	1438.0
24	2242.0
25	3262.0
26	4591.0
27	6335.0
28	8846.0
29	10858.0
30	13925.0
31	17598.0
32	23554.0
33	33399.0
34	50181.0
35	73977.0
36	119550.0
37	171585.0
38	189885.0
39	66931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.65989666956125	0.2646358757785705	0.06397241189736926	0.011495042762808539
8	99.24307642329245	0.5859972886692614	0.08521325178516764	0.08571303625311584
9	94.2489801273201	3.8497148104879773	0.6604651743935428	1.2408398877983868
10	48.61353541285321	32.45725280972581	6.0635101112645176	12.86570166615647
11	30.588808576301467	20.871998950452618	24.530171363599447	24.009021109646465
12	22.351985706164218	16.446157594537354	33.13920871624112	28.062647983057303
13	22.66897400496036	16.806127357577044	32.96053576894964	27.564362868512955
14	21.81996514003336	18.55337385752394	32.1312683905066	27.495392611936104
15	24.08598791771049	18.213520419319167	29.760790659028295	27.93970100394205
16	27.209515896269732	26.34713779682512	22.269021484484817	24.17432482242033
17	30.846072631177808	17.539810956525	22.87276112176624	28.741355290530958
18	29.228520200663468	17.647264617133864	24.231175305649437	28.893039876553235
19	29.326228064147337	17.884662239409256	23.834471384215558	28.954638312227853
20	28.815698230138253	18.85461894557972	24.089111570635165	28.240571253646863
21	32.26083751382217	16.817622400339854	23.420399952520476	27.50114013331751
22	32.314564344126595	16.769518145299838	22.001886686366504	28.91403082420706
23	29.37683124152709	19.276437036527998	23.503489120316864	27.84324260162805
24	30.231712573952485	19.454235361000567	22.706957624524424	27.607094440522523
25	28.418244632002448	19.500590370402765	23.403782118961196	28.67738287863359
26	29.881988392505733	17.86154720776665	22.164066746215696	30.09239765351192
27	30.32717140733059	17.680875122603375	23.592075917260683	28.399877552805354
28	30.406512191617363	19.357152228101633	22.89762539904666	27.338710181234344
29	31.291505538236635	18.288113251160436	20.889866245181764	29.530514965421162
30	29.56350074030574	21.106147973686348	20.643222610249328	28.68712867575858
31	32.38778276868101	20.666462588008923	20.263511360725687	26.682243282584384
32	32.27970437748721	19.627410679144617	21.18348962010133	26.909395323266843
33	31.967214138902598	19.482348237322654	22.777427234505122	25.77301038926963
34	29.675327515009155	21.96540242020629	23.35930130131381	24.999968763470754
35	32.957037277674	21.061667156038958	21.843579956143913	24.137715610143125
36	32.91155689109072	20.606363505738152	20.84301145131162	25.63906815185951
37	31.313621000943343	21.8678195028394	21.458870861940788	25.359688634276466
38	32.8364642747815	20.780663338935085	20.23839719121129	26.144475195072125
39	30.629416064322264	21.92342052489864	21.706264173575146	25.74089923720396
40	29.63097164347875	21.896307217512447	22.74606575914137	25.72665537986743
41	29.04110102518289	20.823145018710683	21.393274150522586	28.74247980558384
42	28.265060692576327	20.971705951808282	23.471128076017216	27.29210527959817
43	27.206892027813005	21.02768181221848	25.538361581567948	26.227064578400565
44	29.231893745822113	19.36564856405675	24.097482960473297	27.304974729647842
45	29.219274188006423	20.08508830566818	23.47200269883613	27.223634807489272
46	28.86955000655967	20.404700472921053	23.844592019691508	26.88115750082777
47	29.435930754861968	20.531145943311945	23.486246556172652	26.546676745653436
48	28.705495754955674	21.648788959761102	23.76537618152172	25.8803391037615
49	28.02304006397241	21.676776889966202	24.73583267216013	25.564350373901256
50	26.555797812193493	22.803166134604453	23.436642947728792	27.20439310547326
51	28.13111845516621	22.460813774059936	22.50716878346213	26.900898987311724
52	28.776465149404316	21.51971962091348	23.892696274731524	25.81111895495068
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	19.5
6	39.0
7	23.5
8	8.0
9	33.0
10	58.0
11	520.5
12	983.0
13	935.0
14	975.0
15	1063.0
16	969.5
17	876.0
18	890.0
19	904.0
20	1048.5
21	1193.0
22	955.0
23	717.0
24	1266.0
25	1815.0
26	2569.5
27	3324.0
28	4417.0
29	5510.0
30	5016.0
31	4522.0
32	4018.0
33	3514.0
34	5162.0
35	6810.0
36	8730.5
37	10651.0
38	9677.5
39	9835.5
40	10967.0
41	12254.0
42	13541.0
43	15026.5
44	16512.0
45	18170.5
46	19829.0
47	21082.0
48	22335.0
49	23811.0
50	25287.0
51	27161.5
52	29036.0
53	32692.0
54	36348.0
55	46248.5
56	56149.0
57	62696.0
58	69243.0
59	77328.0
60	85413.0
61	79439.0
62	73465.0
63	73976.5
64	68551.5
65	62615.0
66	55505.0
67	48395.0
68	39493.0
69	30591.0
70	26589.5
71	22588.0
72	21766.0
73	20944.0
74	20441.0
75	19938.0
76	13546.5
77	7155.0
78	4861.5
79	2568.0
80	1843.0
81	1118.0
82	656.0
83	194.0
84	114.5
85	35.0
86	22.0
87	9.0
88	5.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	800345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.49804406654363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46767985579346	9.646738920271329
2	10.135003685942015	2.7360545273485486
3	4.098673665066024	1.6597223323632928
4	2.217771448110351	1.197423069444632
5	1.3882517559266818	0.9369341688477463
6	0.9952857131175173	0.8060646248676933
7	0.7157930361276554	0.6763264160923305
8	0.5718964166416365	0.6175586426661764
9	0.4731981569042149	0.5748524616690288
>10	4.407291987568921	13.635096960888248
>50	1.8531288411166376	18.05848517142855
>100	1.5837840817308446	37.46414461705133
>500	0.07102584408460981	6.366207746194563
>1k	0.020293098309888517	4.9847938300961365
>5k	9.224135595403871E-4	0.6395965107704029
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5137	0.6418482029624725	No Hit
GAGATCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4953	0.6188581174368554	No Hit
GAGATCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2810	0.35109858873360866	No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2770	0.34610074405412666	No Hit
GAGATCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2735	0.34172762995957995	No Hit
GAGATCGGGATTTCAGGATAATTATCAGAACCAGATATTTCATGATCAAGAG	2682	0.3351054857592663	No Hit
GAGATCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	2166	0.2706332893939489	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG	2011	0.2512666412609562	No Hit
GAGATCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1791	0.22377849552380535	No Hit
GAGATCGGGAGTTTAAATTACATGTGTGAGCACCATTGTGGCTAGTAAGCCA	1717	0.2145324828667637	No Hit
GAGATCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT	1689	0.21103399159112632	No Hit
GAGATCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1584	0.19791464930748615	No Hit
GAGATCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1422	0.17767337835558414	No Hit
GAGATCGGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCT	1328	0.16592844335880153	No Hit
GAGATCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1302	0.16267984431713822	No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1253	0.1565574845847728	No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1202	0.1501852326184333	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG	1168	0.1459370646408736	No Hit
GAGATCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG	1155	0.14431276512004199	No Hit
GAGATCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT	1113	0.13906502820658592	No Hit
GAGATCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1100	0.13744072868575427	No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1072	0.1339422374101169	No Hit
GAGATCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG	1013	0.12657041650788098	No Hit
GAGATCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	975	0.12182246406237311	No Hit
GAGATCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	959	0.11982332619058032	No Hit
GAGATCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	945	0.11807408055276161	No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT	934	0.11669967326590408	No Hit
GAGATCGGAAAATGCAGCTCCTCCACCCCAGCCCACTGTTCGCTCTTGTAGT	891	0.11132699023546094	No Hit
GAGATCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	875	0.10932785236366815	No Hit
GAGATCGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	861	0.10757860672584948	No Hit
GAGATCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	852	0.10645409167296604	No Hit
GAGATCGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGT	849	0.1060792533220049	No Hit
GAGATCGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG	832	0.10395516933322504	No Hit
GAGATCGGGTCGCTTTTCTCTTGAAGGTGGTAGCCATTAGCTTAAGCTGTAG	813	0.1015811931104711	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.6857042900249267	0.0	0.0	0.0	0.0
3	0.6857042900249267	0.0	0.0	0.0	0.0
4	0.6857042900249267	0.0	0.0	0.0	0.0
5	0.6857042900249267	0.0	0.0	0.0	0.0
6	0.6857042900249267	0.0	0.0	0.0	0.0
7	0.686329020609862	0.0	0.0	0.0	0.0
8	0.6874535356627455	0.0	0.0	0.0	0.0
9	0.6880782662476806	0.0	0.0	0.0	0.0
10	0.6945754643310073	0.0	0.0	0.0	0.0
11	0.6999481473614504	0.0	0.0	0.0	0.0
12	0.704321261455997	0.0	0.0	0.0	0.0
13	0.7105685673053496	0.0	0.0	0.0	0.0
14	0.7321842455441091	0.0	0.0	0.0	0.0
15	0.7415552043181378	0.0	0.0	0.0	0.0
16	0.7534250854319074	0.0	0.0	0.0	0.0
17	0.7639205592588196	0.0	0.0	0.0	0.0
18	0.7727917335649002	0.0	0.0	0.0	0.0
19	0.7815379617539936	0.0	0.0	0.0	0.0
20	0.7914087049959705	0.0	0.0	0.0	0.0
21	0.8076517002042869	0.0	0.0	0.0	0.0
22	0.8232699648276681	0.0	0.0	0.0	0.0
23	0.8371389838132306	0.0	0.0	0.0	0.0
24	0.8491338110439873	0.0	0.0	0.0	0.0
25	0.8650019679013425	0.0	0.0	0.0	0.0
26	0.881369909226646	0.0	0.0	0.0	0.0
27	0.8954888204461826	0.0	0.0	0.0	0.0
28	0.9212277205455147	0.0	0.0	0.0	0.0
29	0.9324728710743492	0.0	0.0	0.0	0.0
30	0.9437180216031836	0.0	0.0	0.0	0.0
31	0.9582117711736814	0.0	0.0	0.0	0.0
32	0.96758272994771	0.0	0.0	0.0	0.0
33	0.9782031498916093	0.0	0.0	0.0	0.0
34	0.9878240008996121	0.0	0.0	0.0	0.0
35	1.0115637631271515	0.0	0.0	0.0	0.0
36	1.0214345063691284	0.0	0.0	0.0	0.0
37	1.0326796568979628	0.0	0.0	0.0	0.0
38	1.0424254540229525	0.0	0.0	0.0	0.0
39	1.0531708200838388	0.0	0.0	0.0	0.0
40	1.0616671560389581	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	6.310299E-4	46.000004	31
TTAGGTA	40	5.6079443E-9	46.000004	14
TTAGGGT	20	6.310299E-4	46.000004	14
ACACGCT	20	6.310299E-4	46.000004	45
ACACGAT	20	6.310299E-4	46.000004	28
CGTATCT	20	6.310299E-4	46.000004	33
GTCGACA	40	5.6079443E-9	46.000004	10
AAATAGG	40	5.6079443E-9	46.000004	24
CGACGTC	20	6.310299E-4	46.000004	19
GGTCGAA	20	6.310299E-4	46.000004	18
CATGCGA	40	5.6079443E-9	46.000004	29
CCCTAAT	20	6.310299E-4	46.000004	16
CGGTTAG	20	6.310299E-4	46.000004	14
TTATGCG	20	6.310299E-4	46.000004	14
TATATGA	80	0.0	46.000004	20
ATTCGGT	40	5.6079443E-9	46.000004	10
GTCTAAC	20	6.310299E-4	46.000004	20
GCGTTTA	20	6.310299E-4	46.000004	42
TAACGAA	20	6.310299E-4	46.000004	45
CCTAACA	20	6.310299E-4	46.000004	23
>>END_MODULE