FastQCFastQC Report
Fri 17 Jun 2016
SRR1527058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774824
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC51970.6707329664543173No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC50080.6463403301911144No Hit
GAGATCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT29520.3809897473490754No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG27860.3595655271390664No Hit
GAGATCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG27100.34975684800677315No Hit
GAGATCGGGATTTCAGGATAATTATCAGAACCAGATATTTCATGATCAAGAG25320.3267838889864021No Hit
GAGATCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC21410.2763208160821038No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG20260.26147873581613373No Hit
GAGATCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT17320.2235346349622624No Hit
GAGATCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC15950.20585320021062847No Hit
GAGATCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC15680.20236853788731377No Hit
GAGATCGGGAGTTTAAATTACATGTGTGAGCACCATTGTGGCTAGTAAGCCA15190.1960445210783352No Hit
GAGATCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13960.18016994827212374No Hit
GAGATCGGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCT12770.16481162173603298No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA12210.15758417395434318No Hit
GAGATCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT12190.15732605081928283No Hit
GAGATCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA11790.15216358811807584No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT11750.15164734184795514No Hit
GAGATCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG11480.14816267952464043No Hit
GAGATCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT10970.14158053958060154No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG10580.13654713844692473No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA10340.13344966082620052No Hit
GAGATCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG10140.13086842947559704No Hit
GAGATCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG9770.12609315147698058No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT9340.12054350407318308No Hit
GAGATCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA8810.11370324099408383No Hit
GAGATCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT8660.1117673174811312No Hit
GAGATCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8630.11138013277854067No Hit
GAGATCGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC8440.10892796299546735No Hit
GAGATCGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGT8130.10492705440203194No Hit
GAGATCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG7990.1031201924566095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA253.4161174E-546.09
AACGTCG301.8606133E-646.039
AACGTAT405.6079443E-946.017
AGCGTAT206.310175E-446.014
TCGCATA301.8606133E-646.014
AGCCGAT206.310175E-446.045
CCTTAAA950.046.046
ACACGCT206.310175E-446.045
CATATAA253.4161174E-546.018
CGTATGA405.6079443E-946.019
TTTACGG253.4161174E-546.012
TTTACGA405.6079443E-946.032
CATGCGA301.8606133E-646.029
ACGCACC301.8606133E-646.044
TTTAACG453.092282E-1046.039
CTATATG453.092282E-1046.026
TATGGTA253.4161174E-546.036
CCGTGTA206.310175E-446.037
GTCTCAT301.8606133E-646.030
TAAACGA301.8606133E-646.040
Produced by FastQC (version 0.11.5)