##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527058_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	774824
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39498131188502	33.0	31.0	34.0	31.0	34.0
2	32.2396531341311	33.0	31.0	34.0	31.0	34.0
3	32.775009550556	34.0	31.0	34.0	31.0	34.0
4	36.13764416177093	37.0	35.0	37.0	35.0	37.0
5	36.136301921468615	37.0	37.0	37.0	35.0	37.0
6	36.24912367195647	37.0	37.0	37.0	35.0	37.0
7	36.55600626722972	37.0	37.0	37.0	35.0	37.0
8	36.6613747121927	37.0	37.0	37.0	35.0	37.0
9	38.60897184392843	39.0	39.0	39.0	38.0	39.0
10	38.101086182152336	39.0	39.0	39.0	37.0	39.0
11	37.77328915986082	39.0	38.0	39.0	35.0	39.0
12	37.54318658172695	39.0	37.0	39.0	35.0	39.0
13	37.47384309210866	39.0	37.0	39.0	35.0	39.0
14	38.38714340288892	40.0	38.0	40.0	35.0	40.0
15	38.45979732171435	40.0	38.0	40.0	35.0	40.0
16	38.4998851352049	40.0	38.0	40.0	35.0	40.0
17	38.500072274477816	40.0	38.0	40.0	35.0	40.0
18	38.450409383292204	40.0	38.0	40.0	35.0	40.0
19	38.36190025089569	40.0	38.0	40.0	35.0	40.0
20	38.31725527345565	40.0	38.0	40.0	35.0	40.0
21	33.99570612164827	40.0	35.0	40.0	10.0	40.0
22	34.70117729961901	40.0	35.0	40.0	16.0	40.0
23	36.72468973599166	40.0	35.0	40.0	30.0	40.0
24	37.49686767575604	40.0	37.0	40.0	33.0	40.0
25	37.76065790424664	40.0	37.0	40.0	33.0	40.0
26	37.833622861449825	40.0	38.0	40.0	34.0	40.0
27	37.76498533860593	40.0	37.0	40.0	33.0	40.0
28	37.62945133346412	40.0	37.0	40.0	33.0	40.0
29	37.57050504372606	40.0	37.0	40.0	33.0	40.0
30	37.45952242057551	40.0	37.0	40.0	33.0	40.0
31	37.3153245124054	40.0	36.0	40.0	33.0	40.0
32	37.13732279846778	39.0	36.0	40.0	32.0	40.0
33	36.81415521460357	39.0	35.0	40.0	31.0	40.0
34	36.67209972845446	39.0	35.0	40.0	31.0	40.0
35	36.634913219001994	39.0	35.0	40.0	31.0	40.0
36	36.32311466862152	38.0	35.0	40.0	31.0	40.0
37	36.147729032657736	38.0	35.0	40.0	30.0	40.0
38	36.01616625194883	38.0	35.0	40.0	30.0	40.0
39	35.76463558175792	38.0	35.0	40.0	30.0	40.0
40	30.82621859932062	36.0	29.0	40.0	8.0	40.0
41	31.699536927095703	35.0	29.0	40.0	12.0	40.0
42	33.465935489866084	35.0	30.0	39.0	23.0	40.0
43	34.22825183525549	35.0	33.0	39.0	26.0	40.0
44	34.98867871929625	36.0	34.0	39.0	28.0	40.0
45	35.1949126511311	36.0	34.0	40.0	29.0	40.0
46	35.17767906001879	36.0	34.0	40.0	30.0	40.0
47	35.13468607064314	36.0	34.0	40.0	30.0	40.0
48	34.945604421133055	36.0	34.0	39.0	29.0	40.0
49	34.75645307837651	35.0	34.0	39.0	29.0	40.0
50	34.60946873096342	35.0	34.0	39.0	29.0	40.0
51	34.44198682539518	35.0	34.0	39.0	28.0	40.0
52	33.98788628127162	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	24.0
17	56.0
18	98.0
19	210.0
20	375.0
21	570.0
22	971.0
23	1551.0
24	2311.0
25	3365.0
26	4827.0
27	6710.0
28	9021.0
29	11433.0
30	14442.0
31	19236.0
32	26228.0
33	37610.0
34	59155.0
35	80394.0
36	111375.0
37	130813.0
38	183855.0
39	70186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.67166737220323	0.255025657439625	0.06324016808978555	0.010066802267353618
8	99.25415320124313	0.5872301322622944	0.07588820170774266	0.08272846478684191
9	94.19984409362642	3.8492612515874574	0.6944802948798695	1.2564143599062498
10	48.579677449330426	32.49757364253043	6.06124745748712	12.86150145065202
11	30.630698068206456	20.83686101617916	24.4974342560375	24.035006659576887
12	22.300161068836278	16.50774885651451	33.117585412945395	28.07450466170382
13	22.746714092490684	16.84614828657863	32.9525156680743	27.45462195285639
14	21.81463145178776	18.52317429506572	32.11387360226322	27.548320650883294
15	23.89768515172478	18.072104116547756	29.89259496350139	28.137615768226077
16	28.415614384686073	18.129665575666216	25.381118808916604	28.07360123073111
17	30.87617316964885	17.453770146510692	22.867515719698925	28.80254096414153
18	29.144037871826377	17.589413853984905	24.239827367247273	29.02672090694145
19	29.26780791508781	17.949366565826562	23.78849906559425	28.994326453491375
20	28.829902016457932	18.835116103786152	24.14135339122175	28.193628488534173
21	36.49009323407638	16.665849276738975	22.429997005771632	24.414060483413007
22	32.35405717943688	16.842663624255312	21.94046648012968	28.862812716178126
23	29.27864908676035	19.36839850082083	23.445066234396457	27.907886178022363
24	30.440848502369573	19.39705016881253	22.699090374072046	27.463010954745855
25	28.3870917782619	19.47887520262666	23.489463413626837	28.644569605484598
26	29.88743250080018	17.85218320547634	22.080756404034982	30.179627889688497
27	30.28507119036065	17.71228046627363	23.604715393431285	28.397932949934436
28	30.52706162947973	19.352265804879558	22.8195048165777	27.301167749063016
29	31.17159509772542	18.25743652752109	20.86164083714495	29.709327537608544
30	29.526705419553345	21.14544722414381	20.68301962768319	28.64482772861966
31	32.485699978317655	20.647527696612393	20.17335549750653	26.69341682756342
32	32.26719874448907	19.599289645132316	21.1266042352844	27.006907375094215
33	32.05140780357862	19.516690241913	22.746585030923153	25.685316923585226
34	29.622856287363327	22.091468514139986	23.37459861852498	24.91107657997171
35	32.951225052398996	21.190231588076777	21.77126676509762	24.087276594426605
36	32.96709962520521	20.43315643294477	20.894164352162555	25.705579589687467
37	31.086801647858092	21.874903203824353	21.559476732780606	25.478818415536946
38	32.74369405181047	20.769878062630998	20.384629283553426	26.1017986020051
39	30.398903492922262	21.986283336602895	21.639882089351907	25.974931081122936
40	29.712425015229265	26.541898547282994	20.902295230916955	22.843381206570783
41	28.982969035548717	20.836473831476567	21.449000030974776	28.73155710199994
42	28.247059977491663	20.937399977285164	23.52121255923926	27.294327485983917
43	27.33640155699875	21.07717365492034	25.403317398531794	26.18310738954911
44	29.149845642365236	19.47822989478901	24.123155710199995	27.248768752645763
45	29.1744964017635	20.116310284658194	23.414349581324277	27.294843732254037
46	28.79750756300786	20.43689921840315	23.796629944348652	26.968963274240345
47	29.34576110187604	20.56428298555543	23.420157351863132	26.6697985607054
48	28.76924307971875	21.733451725811282	23.656210958875825	25.84109423559415
49	27.997196782753242	21.723772108246518	24.663794616583896	25.615236492416344
50	26.60591308477796	22.742196937627128	23.43448318585898	27.217406791735932
51	28.1937575501017	22.533117198228243	22.514532332503897	26.75859291916616
52	28.70664821946661	21.613037283305626	23.840510877308912	25.839803619918843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	22.5
6	45.0
7	28.5
8	12.0
9	33.0
10	54.0
11	482.0
12	910.0
13	838.0
14	863.5
15	961.0
16	888.0
17	815.0
18	852.5
19	890.0
20	1078.5
21	1267.0
22	973.5
23	680.0
24	1157.0
25	1634.0
26	2327.0
27	3020.0
28	4126.5
29	5233.0
30	4726.0
31	4219.0
32	3775.0
33	3331.0
34	4691.5
35	6052.0
36	8119.0
37	10186.0
38	9420.5
39	9785.5
40	10916.0
41	12109.0
42	13302.0
43	14616.0
44	15930.0
45	17856.0
46	19782.0
47	20703.0
48	21624.0
49	23154.0
50	24684.0
51	26225.5
52	27767.0
53	31341.0
54	34915.0
55	44537.5
56	54160.0
57	59987.5
58	65815.0
59	74327.0
60	82839.0
61	76955.5
62	71072.0
63	71658.0
64	66469.5
65	60695.0
66	53809.5
67	46924.0
68	38572.0
69	30220.0
70	26316.5
71	22413.0
72	21082.0
73	19751.0
74	19734.0
75	19717.0
76	13441.5
77	7166.0
78	4993.5
79	2821.0
80	1972.0
81	1123.0
82	648.0
83	173.0
84	100.0
85	27.0
86	18.0
87	9.0
88	5.5
89	1.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	774824.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.048415036757667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.84737756766492	10.360439118305813
2	11.793071987476617	3.549340836518175
3	5.416381776935626	2.445238829305749
4	2.8804586140042603	1.7338534687895943
5	1.7968075011202924	1.3519552509008788
6	1.117995930268428	1.009444006085117
7	0.7715203267899386	0.8127110660780932
8	0.5585334658969091	0.6724034725388338
9	0.36950369945890715	0.5004400524367504
>10	3.349480748782321	12.120991045095813
>50	1.6584890400016163	18.159733805100927
>100	1.363399448046713	35.90500874927714
>500	0.057307254089792835	5.639858356875291
>1k	0.01796197516247238	4.425056266997185
>5k	0.001710664301187846	1.313525675694647
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5197	0.6707329664543173	No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5008	0.6463403301911144	No Hit
GAGATCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2952	0.3809897473490754	No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2786	0.3595655271390664	No Hit
GAGATCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2710	0.34975684800677315	No Hit
GAGATCGGGATTTCAGGATAATTATCAGAACCAGATATTTCATGATCAAGAG	2532	0.3267838889864021	No Hit
GAGATCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	2141	0.2763208160821038	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGATCGGGAAG	2026	0.26147873581613373	No Hit
GAGATCGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT	1732	0.2235346349622624	No Hit
GAGATCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1595	0.20585320021062847	No Hit
GAGATCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1568	0.20236853788731377	No Hit
GAGATCGGGAGTTTAAATTACATGTGTGAGCACCATTGTGGCTAGTAAGCCA	1519	0.1960445210783352	No Hit
GAGATCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1396	0.18016994827212374	No Hit
GAGATCGGGGGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACTGCT	1277	0.16481162173603298	No Hit
GAGATCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1221	0.15758417395434318	No Hit
GAGATCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1219	0.15732605081928283	No Hit
GAGATCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1179	0.15216358811807584	No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1175	0.15164734184795514	No Hit
GAGATCGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG	1148	0.14816267952464043	No Hit
GAGATCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT	1097	0.14158053958060154	No Hit
GAGATCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG	1058	0.13654713844692473	No Hit
GAGATCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1034	0.13344966082620052	No Hit
GAGATCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1014	0.13086842947559704	No Hit
GAGATCGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG	977	0.12609315147698058	No Hit
GAGATCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT	934	0.12054350407318308	No Hit
GAGATCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	881	0.11370324099408383	No Hit
GAGATCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	866	0.1117673174811312	No Hit
GAGATCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	863	0.11138013277854067	No Hit
GAGATCGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	844	0.10892796299546735	No Hit
GAGATCGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGT	813	0.10492705440203194	No Hit
GAGATCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	799	0.1031201924566095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.7128070374691543	0.0	0.0	0.0	0.0
3	0.7128070374691543	0.0	0.0	0.0	0.0
4	0.7128070374691543	0.0	0.0	0.0	0.0
5	0.7128070374691543	0.0	0.0	0.0	0.0
6	0.7128070374691543	0.0	0.0	0.0	0.0
7	0.7137104684418655	0.0	0.0	0.0	0.0
8	0.7143557762795164	0.0	0.0	0.0	0.0
9	0.7155173303872879	0.0	0.0	0.0	0.0
10	0.7217122856287363	0.0	0.0	0.0	0.0
11	0.7285525487078356	0.0	0.0	0.0	0.0
12	0.7319081494636202	0.0	0.0	0.0	0.0
13	0.7405552744881418	0.0	0.0	0.0	0.0
14	0.7624957409682716	0.0	0.0	0.0	0.0
15	0.7728206663706855	0.0	0.0	0.0	0.0
16	0.7850815152860521	0.0	0.0	0.0	0.0
17	0.7921799015002117	0.0	0.0	0.0	0.0
18	0.8016013959299144	0.0	0.0	0.0	0.0
19	0.8106357056570266	0.0	0.0	0.0	0.0
20	0.8234128008425139	0.0	0.0	0.0	0.0
21	0.8364480191630616	0.0	0.0	0.0	0.0
22	0.8530969613744541	0.0	0.0	0.0	0.0
23	0.8712946423962087	0.0	0.0	0.0	0.0
24	0.8844589222842865	0.0	0.0	0.0	0.0
25	0.9009788029281488	0.0	0.0	0.0	0.0
26	0.9140140212486965	0.0	0.0	0.0	0.0
27	0.9300176556224381	0.0	0.0	0.0	0.0
28	0.9524743683726885	0.0	0.0	0.0	0.0
29	0.9646061557205249	0.0	0.0	0.0	0.0
30	0.9746729579878786	0.0	0.0	0.0	0.0
31	0.9888697304161977	0.0	0.0	0.0	0.0
32	0.997129670738129	0.0	0.0	0.0	0.0
33	1.005905857330181	0.0	0.0	0.0	0.0
34	1.0154564133274138	0.0	0.0	0.0	0.0
35	1.0379131260776642	0.0	0.0	0.0	0.0
36	1.0479799283450177	0.0	0.0	0.0	0.0
37	1.056239868666949	0.0	0.0	0.0	2.581231350603492E-4
38	1.0650160552590007	0.0	0.0	0.0	2.581231350603492E-4
39	1.0757281653640052	0.0	0.0	0.0	2.581231350603492E-4
40	1.085794967631359	0.0	0.0	0.0	2.581231350603492E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	25	3.4161174E-5	46.0	9
AACGTCG	30	1.8606133E-6	46.0	39
AACGTAT	40	5.6079443E-9	46.0	17
AGCGTAT	20	6.310175E-4	46.0	14
TCGCATA	30	1.8606133E-6	46.0	14
AGCCGAT	20	6.310175E-4	46.0	45
CCTTAAA	95	0.0	46.0	46
ACACGCT	20	6.310175E-4	46.0	45
CATATAA	25	3.4161174E-5	46.0	18
CGTATGA	40	5.6079443E-9	46.0	19
TTTACGG	25	3.4161174E-5	46.0	12
TTTACGA	40	5.6079443E-9	46.0	32
CATGCGA	30	1.8606133E-6	46.0	29
ACGCACC	30	1.8606133E-6	46.0	44
TTTAACG	45	3.092282E-10	46.0	39
CTATATG	45	3.092282E-10	46.0	26
TATGGTA	25	3.4161174E-5	46.0	36
CCGTGTA	20	6.310175E-4	46.0	37
GTCTCAT	30	1.8606133E-6	46.0	30
TAAACGA	30	1.8606133E-6	46.0	40
>>END_MODULE