FastQCFastQC Report
Fri 17 Jun 2016
SRR1527051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143552
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT54730.4785965133199015No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC46170.4037420248488919No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC39860.3485630736512201No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG36600.32005540631296175No Hit
GCCCTCGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAA36330.31769434184016115No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC34430.3010794436982315No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC34220.2992430602193866No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAA31600.27633199014998877No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC30780.26916134989926127No Hit
GCCCTCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG29650.2592798578464294No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG27970.24458879001567047No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA27790.24301474703380344No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT26690.2333955954779494No Hit
GCCCTCGGGCTCTTTCCCACAACTGTGCTGTGTCCTTGTTGCTCTGCTTC25910.22657474255652565No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC22820.19955367136780836No Hit
GCCCTCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG21760.1902843071412581No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG21500.18801068950078353No Hit
GCCCTCGGAGCTGCCCTCGGGGGAGTGTGTGTGACTAACCCTTACTATTT20950.1832011137228565No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT20900.18276387956122678No Hit
GCCCTCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT20410.17847898477725543No Hit
GCCCTCGGGGTTGCACTGCGCTATTTCCCGCGCGGGCTAACCTCGTTCCC19130.16728579023953435No Hit
GCCCTCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA18580.16247621446160734No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT18220.15932812849787328No Hit
GCCCTCGGGGTCCTAAATATCCAAAGACTGTACTGGCCAGTGTCCTTTAC17850.1560925957018133No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA17690.15469344638459817No Hit
GCCCTCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT17460.15268216924110142No Hit
GCCCTCGGGCTCGCTACGTCCAACGCGCCATCAGCTGGAGGCGAGTAGAT17210.15049599843295275No Hit
GCCCTCGGGATGTTGATAATGCTCGGGATATTTTTCAATGTCCATTCTGC16320.14271323035594358No Hit
GCCCTCGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACAC16200.14166386836803224No Hit
GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG16060.140439612715469No Hit
GCCCTCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTC16030.14017727221849116No Hit
GCCCTCGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCA15790.13807854824266846No Hit
GCCCTCGGGGGATATTTATGGCCTGAATACTGCTCTCCTACAGAGAATTC14150.12373726774121334No Hit
GCCCTCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT13840.12102641593910901No Hit
GCCCTCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC13540.11840301096933065No Hit
GCCCTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG13500.11805322364002686No Hit
GCCCTCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC13430.11744109581374525No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC12740.11140726438325498No Hit
GCCCTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG12650.11062024289232146No Hit
GCCCTCCCCTTTCCTTTGATCTTGACCACTCATGGAAGCAGGACCAGTAA12440.10878385941347661No Hit
GCCCTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT12400.10843407208417281No Hit
GCCCTCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC12370.108171731587195No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG12320.10773449742556525No Hit
GCCCTCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG12110.10589811394672038No Hit
GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA11610.1015257723304231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.8564644E-444.00000411
CTAGACG207.8564644E-444.00000442
CGGGTAT408.312782E-944.0000046
CTATGCG207.8564644E-444.00000430
TGATACG408.312782E-944.00000414
GATACGG408.312782E-944.00000415
ATCTACG408.312782E-944.00000426
ACGCATT207.8564644E-444.00000413
GCGAATA207.8564644E-444.00000438
CTATACA207.8564644E-444.00000438
ATTACCT207.8564644E-444.00000420
ATTCGAT207.8564644E-444.00000428
TACGGTG408.312782E-944.00000429
CAATTAA207.8564644E-444.00000436
TTATAGC408.312782E-944.00000436
CGTTAAC650.044.00000412
GTTACGA207.8564644E-444.00000444
CCGTAAA207.8564644E-444.00000430
GCCGAAT207.8564644E-444.00000443
TCTACGA207.8564644E-444.00000425