##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143552
Sequences flagged as poor quality	0
Sequence length	50
%GC	62
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27710502014775	33.0	31.0	34.0	31.0	34.0
2	32.37364719750392	34.0	31.0	34.0	31.0	34.0
3	32.85191840860757	34.0	31.0	34.0	31.0	34.0
4	36.48846051600627	37.0	37.0	37.0	35.0	37.0
5	36.07652996977838	37.0	35.0	37.0	35.0	37.0
6	36.3033827932617	37.0	37.0	37.0	35.0	37.0
7	36.38467861540184	37.0	37.0	37.0	35.0	37.0
8	36.57178510465637	37.0	37.0	37.0	35.0	37.0
9	38.69369998041191	39.0	39.0	39.0	39.0	39.0
10	38.11228435611149	39.0	39.0	39.0	37.0	39.0
11	37.66271232090889	39.0	37.0	39.0	35.0	39.0
12	37.03645483546004	39.0	37.0	39.0	34.0	39.0
13	36.79561664008283	39.0	37.0	39.0	33.0	39.0
14	37.57698032096485	40.0	37.0	40.0	33.0	40.0
15	37.611932819845535	40.0	37.0	40.0	33.0	40.0
16	37.48551530669353	40.0	37.0	40.0	33.0	40.0
17	37.549599843295276	40.0	37.0	40.0	33.0	40.0
18	37.54205055826058	40.0	37.0	40.0	33.0	40.0
19	37.379643426796505	39.0	36.0	40.0	33.0	40.0
20	37.12628721737184	39.0	36.0	40.0	32.0	40.0
21	37.23007523925453	39.0	36.0	40.0	32.0	40.0
22	37.10056123376987	39.0	36.0	40.0	32.0	40.0
23	37.08022547291247	39.0	36.0	40.0	32.0	40.0
24	37.08709442159167	39.0	36.0	40.0	32.0	40.0
25	36.99218924893665	39.0	35.0	40.0	32.0	40.0
26	36.73906215021267	39.0	35.0	40.0	31.0	40.0
27	36.63854813773226	38.0	35.0	40.0	31.0	40.0
28	36.333031641761806	38.0	35.0	40.0	30.0	40.0
29	36.788086593351245	39.0	35.0	40.0	31.0	40.0
30	36.84350427440116	39.0	35.0	40.0	32.0	40.0
31	36.76091161573763	39.0	35.0	40.0	31.0	40.0
32	36.021773386780836	39.0	35.0	40.0	30.0	40.0
33	36.17094106783076	39.0	35.0	40.0	30.0	40.0
34	36.08255418205731	38.0	35.0	40.0	30.0	40.0
35	36.07389956906201	38.0	35.0	40.0	30.0	40.0
36	35.80643643664652	38.0	35.0	40.0	30.0	40.0
37	35.63632436478621	38.0	35.0	40.0	29.0	40.0
38	35.39741524653011	38.0	34.0	40.0	28.0	40.0
39	35.29991115401835	37.0	34.0	40.0	28.0	40.0
40	35.14221041107007	37.0	34.0	40.0	28.0	40.0
41	34.99891303587419	37.0	34.0	40.0	27.0	40.0
42	34.89044485952541	37.0	34.0	40.0	27.0	40.0
43	34.73295398897471	36.0	34.0	40.0	27.0	40.0
44	34.591249895063804	36.0	34.0	40.0	27.0	40.0
45	34.42755816963286	35.0	33.0	40.0	26.0	40.0
46	34.117642223528094	35.0	33.0	39.0	25.0	40.0
47	34.00818327456906	35.0	33.0	39.0	25.0	40.0
48	33.83436258254981	35.0	33.0	39.0	25.0	40.0
49	33.52572860700694	35.0	33.0	39.0	24.0	40.0
50	33.08407051012984	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	30.0
16	69.0
17	128.0
18	217.0
19	458.0
20	757.0
21	1376.0
22	2247.0
23	3244.0
24	4798.0
25	6639.0
26	8901.0
27	12176.0
28	16598.0
29	20561.0
30	25541.0
31	30897.0
32	38695.0
33	53426.0
34	88285.0
35	100168.0
36	141262.0
37	207597.0
38	289832.0
39	89642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.71846667226326	0.5205709928363554	0.24336453436310723	0.5175978005372733
8	98.63915239534363	0.6670444369823147	0.359931161853593	0.33387200582046117
9	97.4253903626595	1.2758492836355495	0.38327946608462055	0.9154808876203269
10	62.71949154913813	25.599448035594357	4.128802188269532	7.552258226997985
11	34.142828660174615	25.714702960599954	20.106387816207747	20.036080563017684
12	31.798641426012985	14.643496753973583	27.763407348332215	25.794454471681217
13	22.83053153682561	16.12694481755093	31.438185583165435	29.604338062458023
14	24.016048242668457	17.408915379449294	31.324067466980075	27.250968910902174
15	24.598444145959256	17.469341140586522	29.47806483657936	28.45414987687486
16	27.968120382807253	18.883618759794047	25.754578716140585	27.393682141258115
17	30.816088817998654	18.34407180434296	22.89629155473472	27.943547822923666
18	29.56402507275576	18.739943614282517	23.545496837922546	28.150534475039173
19	29.240122005820464	18.15492430602194	23.665386445041413	28.939567243116187
20	30.59222492724424	19.44039274121334	22.75462768636669	27.212754645175735
21	30.38917338258339	18.26204667562122	25.54120844526528	25.80757149653011
22	31.366828967987466	17.461383478844862	23.952037161405865	27.21975039176181
23	28.59030459480636	19.971719694425786	24.21052999776136	27.227445713006492
24	28.997544492948286	19.907796059995523	23.263743144168345	27.830916302887843
25	28.43753497873293	20.16576421535706	21.945919381016342	29.450781424893663
26	28.96772512312514	20.30751553055742	22.205636473024402	28.51912287329304
27	30.906421395791362	18.022442355048128	22.382978648981418	28.688157600179093
28	29.657505736512203	20.544846233490038	21.090514467203942	28.707133562793825
29	30.459218295276475	18.681704023953436	21.854624888068056	29.004452792702036
30	28.573689696664427	19.57138809603761	22.311622033803445	29.543300173494515
31	30.65448707186031	19.046269867920305	21.99217875531677	28.307064304902617
32	32.14458109469442	19.911031592791584	22.754452792702036	25.189934519811953
33	32.93964769420192	19.796651136109244	21.306770483546003	25.956930686142826
34	31.12853634989926	20.963366773002015	22.538459116856952	25.369637760241776
35	30.269546115961493	22.34310289344079	23.38678083725095	24.000570153346764
36	31.26731447280054	21.86957829639579	22.49228718938885	24.37082004141482
37	30.15280459480636	22.02724493507947	21.447909670920083	26.372040799194092
38	33.44788868368032	21.370781564808595	20.965552943810163	24.215776807700916
39	31.052545052608018	21.28718239310499	21.618605887620326	26.041666666666668
40	30.95836481419297	21.038658495634653	21.476067550929034	26.526909139243337
41	28.274184295948064	20.783401192075218	24.067466980076112	26.874947531900606
42	27.76952862659503	20.43238960711887	22.8894707018133	28.9086110644728
43	27.076162693082605	18.650485504813076	25.753879141481978	28.51947266062234
44	27.39726746138348	19.60820321244683	24.909492528542646	28.085036797627044
45	28.27811940340273	17.95344680434296	26.221369907096488	27.547063885157826
46	29.20147050593239	19.241626091336467	24.353855355943583	27.203048046787554
47	27.305972968435192	20.535926656592792	25.33544604880233	26.822654326169687
48	29.574081458473252	19.68279536042086	24.57535818222521	26.16776499888068
49	28.21603215245131	21.75598486120439	24.241748516901723	25.786234469442583
50	25.674477417730017	22.006694929482876	23.794982650548466	28.52384500223864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	41.5
12	81.0
13	47.5
14	14.0
15	148.5
16	283.0
17	415.0
18	547.0
19	741.0
20	935.0
21	1218.5
22	1502.0
23	1487.0
24	1472.0
25	1749.5
26	2027.0
27	2084.0
28	2141.0
29	2434.0
30	2727.0
31	3629.0
32	4531.0
33	5215.5
34	5900.0
35	6523.5
36	7147.0
37	7740.5
38	8334.0
39	8946.0
40	9558.0
41	11418.0
42	13278.0
43	14687.0
44	16096.0
45	19807.5
46	23519.0
47	23103.5
48	22688.0
49	25683.5
50	28679.0
51	30653.5
52	32628.0
53	37041.0
54	41454.0
55	45105.5
56	48757.0
57	55825.0
58	62893.0
59	67756.0
60	72619.0
61	85813.5
62	99008.0
63	106909.5
64	114811.0
65	113437.0
66	112063.0
67	110747.0
68	109431.0
69	102562.0
70	95693.0
71	79107.0
72	62521.0
73	54546.0
74	46571.0
75	40062.5
76	33554.0
77	28106.5
78	22659.0
79	22567.0
80	22475.0
81	15339.5
82	8204.0
83	5922.5
84	3641.0
85	3072.5
86	2504.0
87	1422.5
88	341.0
89	232.0
90	123.0
91	110.5
92	98.0
93	63.0
94	28.0
95	18.5
96	9.0
97	7.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1143552.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.041223165790765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64099992073238	7.093220448586816
2	11.34341282644941	2.278034793041438
3	4.537089881167763	1.3667379606016983
4	2.2422615297754582	0.9006019366617108
5	1.31846189570815	0.6619485065198542
6	0.7707194199453804	0.4643379416328225
7	0.5678896843094766	0.3991614937591345
8	0.35360743290672525	0.28405209175190504
9	0.2821803788049616	0.2550092540929195
>10	2.9893481516282274	7.743426219355171
>50	2.300651716659746	16.906315288229102
>100	2.490241643482991	44.443682184418705
>500	0.11375395609394681	7.440868385818904
>1k	0.048499748722225386	9.27799740669081
>5k	8.818136131313706E-4	0.48460608883901785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5473	0.4785965133199015	No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4617	0.4037420248488919	No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3986	0.3485630736512201	No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3660	0.32005540631296175	No Hit
GCCCTCGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAA	3633	0.31769434184016115	No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	3443	0.3010794436982315	No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3422	0.2992430602193866	No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAA	3160	0.27633199014998877	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3078	0.26916134989926127	No Hit
GCCCTCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	2965	0.2592798578464294	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2797	0.24458879001567047	No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2779	0.24301474703380344	No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	2669	0.2333955954779494	No Hit
GCCCTCGGGCTCTTTCCCACAACTGTGCTGTGTCCTTGTTGCTCTGCTTC	2591	0.22657474255652565	No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2282	0.19955367136780836	No Hit
GCCCTCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG	2176	0.1902843071412581	No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2150	0.18801068950078353	No Hit
GCCCTCGGAGCTGCCCTCGGGGGAGTGTGTGTGACTAACCCTTACTATTT	2095	0.1832011137228565	No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	2090	0.18276387956122678	No Hit
GCCCTCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	2041	0.17847898477725543	No Hit
GCCCTCGGGGTTGCACTGCGCTATTTCCCGCGCGGGCTAACCTCGTTCCC	1913	0.16728579023953435	No Hit
GCCCTCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA	1858	0.16247621446160734	No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1822	0.15932812849787328	No Hit
GCCCTCGGGGTCCTAAATATCCAAAGACTGTACTGGCCAGTGTCCTTTAC	1785	0.1560925957018133	No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1769	0.15469344638459817	No Hit
GCCCTCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1746	0.15268216924110142	No Hit
GCCCTCGGGCTCGCTACGTCCAACGCGCCATCAGCTGGAGGCGAGTAGAT	1721	0.15049599843295275	No Hit
GCCCTCGGGATGTTGATAATGCTCGGGATATTTTTCAATGTCCATTCTGC	1632	0.14271323035594358	No Hit
GCCCTCGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACAC	1620	0.14166386836803224	No Hit
GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	1606	0.140439612715469	No Hit
GCCCTCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTC	1603	0.14017727221849116	No Hit
GCCCTCGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCA	1579	0.13807854824266846	No Hit
GCCCTCGGGGGATATTTATGGCCTGAATACTGCTCTCCTACAGAGAATTC	1415	0.12373726774121334	No Hit
GCCCTCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1384	0.12102641593910901	No Hit
GCCCTCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1354	0.11840301096933065	No Hit
GCCCTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1350	0.11805322364002686	No Hit
GCCCTCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAAC	1343	0.11744109581374525	No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1274	0.11140726438325498	No Hit
GCCCTCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1265	0.11062024289232146	No Hit
GCCCTCCCCTTTCCTTTGATCTTGACCACTCATGGAAGCAGGACCAGTAA	1244	0.10878385941347661	No Hit
GCCCTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	1240	0.10843407208417281	No Hit
GCCCTCGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	1237	0.108171731587195	No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1232	0.10773449742556525	No Hit
GCCCTCGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	1211	0.10589811394672038	No Hit
GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1161	0.1015257723304231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.7489366465189165E-4	0.0	0.0	0.0	0.0
8	3.497873293037833E-4	0.0	0.0	0.0	0.0
9	5.24680993955675E-4	0.0	0.0	0.0	0.0
10	0.0034104264607118873	0.0	0.0	0.0	0.0
11	0.008482342735616745	0.0	0.0	0.0	0.0
12	0.011630428699350794	0.0	0.0	0.0	0.0
13	0.01591532348332214	0.0	0.0	0.0	0.0
14	0.02929468882919185	0.0	0.0	0.0	0.0
15	0.040138096037609136	0.0	0.0	0.0	0.0
16	0.05001958809044101	0.0	0.0	0.0	0.0
17	0.058064696664428025	0.0	0.0	0.0	0.0
18	0.06331150660398478	0.0	0.0	0.0	0.0
19	0.07135661517797179	0.0	0.0	0.0	0.0
20	0.0765159782852026	0.0	0.0	0.0	0.0
21	0.08062597940452206	0.0	0.0	0.0	0.0
22	0.08543555518244907	0.0	0.0	0.0	0.0
23	0.09269364226550257	0.0	0.0	0.0	0.0
24	0.10126343183344527	0.0	0.0	0.0	0.0
25	0.10563577344974255	0.0	0.0	0.0	0.0
26	0.11271896686814417	0.0	0.0	0.0	0.0
27	0.12190088426236848	0.0	0.0	0.0	0.0
28	0.172707493843743	0.0	0.0	0.0	0.0
29	0.17891621893888515	0.0	0.0	0.0	0.0
30	0.19212069062010298	0.0	0.0	0.0	0.0
31	0.20016579919409	0.0	0.0	0.0	0.0
32	0.20908537609133646	0.0	0.0	0.0	0.0
33	0.21398239870158944	0.0	0.0	0.0	0.0
34	0.21957899597044997	0.0	0.0	0.0	0.0
35	0.25315857958361315	0.0	0.0	0.0	0.0
36	0.26216560331318556	0.0	0.0	0.0	0.0
37	0.2707353928811283	0.0	0.0	0.0	0.0
38	0.2780809267965077	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.8564644E-4	44.000004	11
CTAGACG	20	7.8564644E-4	44.000004	42
CGGGTAT	40	8.312782E-9	44.000004	6
CTATGCG	20	7.8564644E-4	44.000004	30
TGATACG	40	8.312782E-9	44.000004	14
GATACGG	40	8.312782E-9	44.000004	15
ATCTACG	40	8.312782E-9	44.000004	26
ACGCATT	20	7.8564644E-4	44.000004	13
GCGAATA	20	7.8564644E-4	44.000004	38
CTATACA	20	7.8564644E-4	44.000004	38
ATTACCT	20	7.8564644E-4	44.000004	20
ATTCGAT	20	7.8564644E-4	44.000004	28
TACGGTG	40	8.312782E-9	44.000004	29
CAATTAA	20	7.8564644E-4	44.000004	36
TTATAGC	40	8.312782E-9	44.000004	36
CGTTAAC	65	0.0	44.000004	12
GTTACGA	20	7.8564644E-4	44.000004	44
CCGTAAA	20	7.8564644E-4	44.000004	30
GCCGAAT	20	7.8564644E-4	44.000004	43
TCTACGA	20	7.8564644E-4	44.000004	25
>>END_MODULE