FastQCFastQC Report
Fri 17 Jun 2016
SRR1527050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239632
Sequences flagged as poor quality0
Sequence length52
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG54470.4394045974934497No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA42300.34123030060534093No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT41780.3370355073118474No Hit
GCCCTCGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAATT36260.2925061631193773No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG35180.28379390012519845No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG32510.262255249945145No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATA32270.2603191915019941No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC31070.2506388992862398No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC28400.22910024910618634No Hit
GCCCTCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA27820.22442144120190508No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC27340.22054932431560334No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT26320.21232107593221217No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC24190.19513855724924817No Hit
GCCCTCGGGCTCTTTCCCACAACTGTGCTGTGTCCTTGTTGCTCTGCTTCCT23750.19158911677013823No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC22050.17787536946448623No Hit
GCCCTCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA21570.1740032525781845No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC21300.17182518682963976No Hit
GCCCTCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG19690.15883746144016933No Hit
GCCCTCGGGGTCCTAAATATCCAAAGACTGTACTGGCCAGTGTCCTTTACTT19220.15504601365566556No Hit
GCCCTCGGAGCTGCCCTCGGGGGAGTGTGTGTGACTAACCCTTACTATTTAA19010.15335196251790856No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18970.15302928611071673No Hit
GCCCTCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC18620.1502058675477884No Hit
GCCCTCGGGCTCGCTACGTCCAACGCGCCATCAGCTGGAGGCGAGTAGATGC18200.14681776527227436No Hit
GCCCTCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG18190.1467370961704764No Hit
GCCCTCGGGGTTGCACTGCGCTATTTCCCGCGCGGGCTAACCTCGTTCCCAG17690.14270364108057876No Hit
GCCCTCGGGATGTTGATAATGCTCGGGATATTTTTCAATGTCCATTCTGCTG17140.13826684048169133No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17030.13737948036191386No Hit
GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT16930.13657278934393433No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA16390.1322166578468449No Hit
GCCCTCGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA16020.12923190108032062No Hit
GCCCTCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTG15430.12447242407424139No Hit
GCCCTCGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAA14950.12060030718793965No Hit
GCCCTCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA13560.1093873020380242No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT13210.10656388347509582No Hit
GCCCTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12810.10333711940317772No Hit
GCCCTCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC12660.10212708287620842No Hit
GCCCTCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC12510.10091704634923913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA351.0196527E-746.0000046
GATGCGT351.0196527E-746.00000418
GTCATAC301.8615228E-646.011
CTATGCG206.311648E-446.030
ATCTCGT206.311648E-446.010
ACACGTA405.6115823E-946.014
ATTAGAC206.311648E-446.038
AATCACG206.311648E-446.022
CAACGTA206.311648E-446.043
CGAAAAT206.311648E-446.046
GACGTAT206.311648E-446.010
CCAATTT206.311648E-446.018
CCAATTA301.8615228E-646.041
TTCACGG1250.046.029
GTGATAG206.311648E-446.040
ATTCGAT253.4173114E-546.028
CGATTCC301.8615228E-646.012
TCGATAG253.4173114E-546.043
TTATAGC253.4173114E-546.036
AAACGTT253.4173114E-546.039
Produced by FastQC (version 0.11.5)