##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239632
Sequences flagged as poor quality	0
Sequence length	52
%GC	62
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35698174942241	33.0	31.0	34.0	31.0	34.0
2	32.41893642629425	34.0	31.0	34.0	31.0	34.0
3	32.88661070382178	34.0	31.0	34.0	31.0	34.0
4	36.43952479445513	37.0	37.0	37.0	35.0	37.0
5	36.08916436490829	37.0	37.0	37.0	35.0	37.0
6	36.33102969268299	37.0	37.0	37.0	35.0	37.0
7	36.39476070317642	37.0	37.0	37.0	35.0	37.0
8	36.549525988357836	37.0	37.0	37.0	35.0	37.0
9	38.664527859881	39.0	39.0	39.0	38.0	39.0
10	38.043381422873885	39.0	38.0	39.0	37.0	39.0
11	37.66090420382823	39.0	37.0	39.0	35.0	39.0
12	37.120665649160394	39.0	37.0	39.0	34.0	39.0
13	36.95517298682189	39.0	37.0	39.0	33.0	39.0
14	37.781675529511986	40.0	38.0	40.0	33.0	40.0
15	37.716919214734695	40.0	37.0	40.0	33.0	40.0
16	32.72518941105102	39.0	33.0	40.0	3.0	40.0
17	34.34189178724009	39.0	34.0	40.0	17.0	40.0
18	36.33832298617654	39.0	34.0	40.0	30.0	40.0
19	37.149783161454366	39.0	36.0	40.0	32.0	40.0
20	37.431232010790296	39.0	36.0	40.0	33.0	40.0
21	37.57649286239787	40.0	37.0	40.0	33.0	40.0
22	37.64710252720162	40.0	37.0	40.0	33.0	40.0
23	37.582530137976434	40.0	37.0	40.0	33.0	40.0
24	37.524704912425626	40.0	36.0	40.0	33.0	40.0
25	37.4569767479381	40.0	36.0	40.0	33.0	40.0
26	37.23332488996734	39.0	36.0	40.0	33.0	40.0
27	37.15920369916233	39.0	35.0	40.0	32.0	40.0
28	36.93252755656518	39.0	35.0	40.0	32.0	40.0
29	36.76129690101578	39.0	35.0	40.0	31.0	40.0
30	36.68514768899157	39.0	35.0	40.0	31.0	40.0
31	36.57533606747809	39.0	35.0	40.0	31.0	40.0
32	36.41961808020445	38.0	35.0	40.0	31.0	40.0
33	36.087432399292695	38.0	35.0	40.0	30.0	40.0
34	35.9401290060276	38.0	35.0	40.0	30.0	40.0
35	35.82700430450327	38.0	34.0	40.0	30.0	40.0
36	35.65891006363179	38.0	34.0	40.0	30.0	40.0
37	35.245634994901714	37.0	34.0	40.0	28.0	40.0
38	35.17649270105967	37.0	34.0	40.0	28.0	40.0
39	34.75909786130077	37.0	33.0	40.0	27.0	40.0
40	34.65763952527847	36.0	33.0	40.0	26.0	40.0
41	34.585860158498654	36.0	33.0	39.0	26.0	40.0
42	34.52669824593105	36.0	33.0	39.0	27.0	40.0
43	34.36070704854344	35.0	33.0	39.0	26.0	40.0
44	34.776821669656805	36.0	34.0	39.0	28.0	40.0
45	34.8598930973063	35.0	34.0	40.0	28.0	40.0
46	34.772049285594434	35.0	34.0	39.0	29.0	40.0
47	34.50594127934742	35.0	34.0	39.0	28.0	40.0
48	34.43016233850046	35.0	34.0	39.0	28.0	40.0
49	34.34312360442454	35.0	33.0	39.0	28.0	40.0
50	33.8658246963615	35.0	33.0	39.0	26.0	40.0
51	33.90033574480168	35.0	33.0	38.0	26.0	40.0
52	33.48063618880442	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	14.0
16	35.0
17	96.0
18	198.0
19	405.0
20	745.0
21	1283.0
22	2235.0
23	3317.0
24	5041.0
25	7212.0
26	9893.0
27	13839.0
28	17101.0
29	21183.0
30	26893.0
31	34451.0
32	46817.0
33	66912.0
34	100463.0
35	127373.0
36	179818.0
37	240602.0
38	248710.0
39	84992.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.7544690682396	0.512652141925991	0.23377905701046764	0.4990997328239348
8	98.64233901674045	0.6758457348632497	0.36494701653393913	0.31686823186235913
9	97.50377531396414	1.2422234985866774	0.37285258851013847	0.8811485989390401
10	62.819449643119896	25.479416471985232	4.115253559120771	7.585880325774101
11	34.226609187242666	25.70391858228894	19.964311989364585	20.10516024110381
12	31.72812576635647	14.630470978483936	27.68878183202757	25.952621423132026
13	22.90599145552874	16.00652451695342	31.359306632936224	29.728177394581618
14	24.129096376989303	17.324012287517583	31.243062457245372	27.30382887824774
15	24.608028834363747	17.483172425364945	29.42357086619255	28.48522787407876
16	26.642987596318907	26.781819120513184	22.91615576235528	23.65903752081263
17	30.980726538198432	18.29236418550021	22.612517263187783	28.11439201311357
18	29.81247660596048	18.657230532932353	23.425581140209353	28.10471172089781
19	29.491494249906424	18.062215238070653	23.63443344476425	28.81185706725867
20	30.586657975915433	19.54120255043432	22.74788001600475	27.124259457645493
21	30.452263252320044	18.243962724421444	25.563231668753307	25.74054235450521
22	31.191434232094682	17.470184699975476	23.974292370639027	27.36408869729081
23	28.842027311331105	19.919056623255933	24.136275927049315	27.102640138363643
24	28.84460872258864	20.00190379080243	23.180750416252565	27.972737070356363
25	28.604134130128944	20.115969900744737	21.84785484724499	29.43204112188133
26	28.991749164268104	20.226405901106133	22.192069904616854	28.58977503000891
27	30.952895698078137	17.925723117828518	22.18593905288021	28.935442131213136
28	29.82901377182906	20.434854860152047	21.081175703757243	28.65495566426165
29	30.870935890651417	18.617379996644164	21.75798946784207	28.75369464486235
30	28.63083560282406	19.541041212230724	22.186503736592794	29.64161944835241
31	30.83713553699808	18.857451243594873	21.90997005562941	28.39544316377764
32	32.23795448971953	19.51248435019425	22.825241684628985	25.42431947545723
33	33.20412832195361	19.73359835822244	21.384330188313953	25.677943131509995
34	31.144646153051873	20.82400260722537	22.62736037791861	25.40399086180415
35	30.478480710404376	22.31565496857132	23.365724666675273	23.84013965434903
36	31.316874685390506	21.80042141538779	22.424154910489566	24.45854898873214
37	30.29310311447268	22.078891154794324	21.337622778372936	26.29038295236005
38	33.49550511764782	21.297852911186546	20.90918917872401	24.29745279244163
39	31.2853330665875	21.253404236095875	21.510577332627747	25.950685364688873
40	30.977338435922917	21.020109193696193	21.445316029273204	26.557236341107682
41	28.395281825574042	20.688962530815598	24.04600720213741	26.86974844147295
42	27.75735056855583	20.32708094014998	22.85541192870142	29.06015656259277
43	27.067145733572545	18.59987480155401	25.689640151270698	28.643339313602745
44	27.469684551544326	19.383010441808537	24.924735728022508	28.222569278624626
45	28.261693792996635	17.78963434309537	26.2494030486467	27.699268815261302
46	28.775071956838804	19.197874853182235	24.58310208190818	27.44395110807078
47	27.489609819688425	20.52496224686036	25.352201298449863	26.633226635001357
48	29.700588561766715	19.56467725905753	24.40845347651561	26.326280702660142
49	28.17150573718652	21.781867521974263	24.21823573447604	25.828391006363177
50	25.594047265640125	22.157704867250928	23.699856086322395	28.54839178078656
51	26.675819920750676	22.290324870606764	24.28430372884856	26.749551479794004
52	28.55976612414007	22.81249596654491	21.985234327606904	26.64250358170812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	44.5
12	89.0
13	57.5
14	150.5
15	275.0
16	425.0
17	575.0
18	758.0
19	941.0
20	1256.0
21	1571.0
22	1497.0
23	1423.0
24	1669.0
25	1915.0
26	1947.5
27	1980.0
28	2051.0
29	2122.0
30	3364.5
31	4607.0
32	4654.5
33	4702.0
34	5912.0
35	7122.0
36	7733.0
37	8344.0
38	7767.0
39	10511.0
40	13832.0
41	13660.5
42	13489.0
43	16154.5
44	18820.0
45	20285.0
46	21750.0
47	23748.0
48	25746.0
49	28579.0
50	31412.0
51	32210.5
52	33009.0
53	38046.0
54	43083.0
55	47381.0
56	51679.0
57	59648.5
58	67618.0
59	73447.0
60	79276.0
61	88057.5
62	96839.0
63	109514.0
64	117723.0
65	113257.0
66	116502.5
67	119748.0
68	111638.5
69	103529.0
70	89575.0
71	75621.0
72	63673.0
73	51725.0
74	44078.5
75	36432.0
76	34198.0
77	31964.0
78	28426.5
79	24889.0
80	18426.5
81	11964.0
82	8520.0
83	5076.0
84	4072.0
85	3068.0
86	1768.0
87	468.0
88	316.0
89	113.5
90	63.0
91	45.0
92	27.0
93	19.0
94	11.0
95	6.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1239632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.394467392482955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63192562417156	8.276021081778701
2	10.127518217751206	2.307953521978864
3	4.007331746347589	1.3698423274385814
4	2.0771144094335865	0.9467044963498996
5	1.2292820315798547	0.7003507012500929
6	0.8083138129642037	0.5526183230828511
7	0.5651770565375827	0.4507924079187887
8	0.43813571051603056	0.399385845356581
9	0.3300876967611222	0.3385056147663968
>10	3.488083619784446	9.891453288320394
>50	2.083179635175513	17.39123726353152
>100	2.082424312610185	41.75984879745197
>500	0.09290467553538365	6.876678239794091
>1k	0.037766128266416114	8.269812126323007
>5k	7.553225653283222E-4	0.46879596465824475
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5447	0.4394045974934497	No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4230	0.34123030060534093	No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4178	0.3370355073118474	No Hit
GCCCTCGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAATT	3626	0.2925061631193773	No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3518	0.28379390012519845	No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3251	0.262255249945145	No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATA	3227	0.2603191915019941	No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3107	0.2506388992862398	No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2840	0.22910024910618634	No Hit
GCCCTCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	2782	0.22442144120190508	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2734	0.22054932431560334	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2632	0.21232107593221217	No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2419	0.19513855724924817	No Hit
GCCCTCGGGCTCTTTCCCACAACTGTGCTGTGTCCTTGTTGCTCTGCTTCCT	2375	0.19158911677013823	No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2205	0.17787536946448623	No Hit
GCCCTCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA	2157	0.1740032525781845	No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2130	0.17182518682963976	No Hit
GCCCTCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	1969	0.15883746144016933	No Hit
GCCCTCGGGGTCCTAAATATCCAAAGACTGTACTGGCCAGTGTCCTTTACTT	1922	0.15504601365566556	No Hit
GCCCTCGGAGCTGCCCTCGGGGGAGTGTGTGTGACTAACCCTTACTATTTAA	1901	0.15335196251790856	No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1897	0.15302928611071673	No Hit
GCCCTCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	1862	0.1502058675477884	No Hit
GCCCTCGGGCTCGCTACGTCCAACGCGCCATCAGCTGGAGGCGAGTAGATGC	1820	0.14681776527227436	No Hit
GCCCTCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG	1819	0.1467370961704764	No Hit
GCCCTCGGGGTTGCACTGCGCTATTTCCCGCGCGGGCTAACCTCGTTCCCAG	1769	0.14270364108057876	No Hit
GCCCTCGGGATGTTGATAATGCTCGGGATATTTTTCAATGTCCATTCTGCTG	1714	0.13826684048169133	No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1703	0.13737948036191386	No Hit
GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1693	0.13657278934393433	No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1639	0.1322166578468449	No Hit
GCCCTCGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA	1602	0.12923190108032062	No Hit
GCCCTCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTG	1543	0.12447242407424139	No Hit
GCCCTCGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAA	1495	0.12060030718793965	No Hit
GCCCTCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	1356	0.1093873020380242	No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1321	0.10656388347509582	No Hit
GCCCTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1281	0.10333711940317772	No Hit
GCCCTCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC	1266	0.10212708287620842	No Hit
GCCCTCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	1251	0.10091704634923913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.226764071918118E-4	0.0	0.0	0.0	0.0
8	4.033455089897647E-4	0.0	0.0	0.0	0.0
9	5.646837125856706E-4	0.0	0.0	0.0	0.0
10	0.0029040876647263058	0.0	0.0	0.0	0.0
11	0.008712262994178918	0.0	0.0	0.0	0.0
12	0.0109709978445216	0.0	0.0	0.0	0.0
13	0.015246460239813105	0.0	0.0	0.0	0.0
14	0.02904087664726306	0.0	0.0	0.0	0.0
15	0.03855983065942151	0.0	0.0	0.0	0.0
16	0.04961149760574106	0.0	0.0	0.0	0.0
17	0.05509699652800186	0.0	0.0	0.0	0.0
18	0.058727106108909743	0.0	0.0	0.0	0.0
19	0.06437394323476645	0.0	0.0	0.0	0.0
20	0.07018211856421906	0.0	0.0	0.0	0.0
21	0.07469958826490443	0.0	0.0	0.0	0.0
22	0.08042709449255908	0.0	0.0	0.0	0.0
23	0.08558991700762807	0.0	0.0	0.0	0.0
24	0.09397950359461518	0.0	0.0	0.0	0.0
25	0.09946500251687597	0.0	0.0	0.0	0.0
26	0.10519250874453064	0.0	0.0	0.0	0.0
27	0.11511480826567885	0.0	0.0	0.0	0.0
28	0.1674690553325503	0.0	0.0	0.0	0.0
29	0.17319656156020496	0.0	0.0	0.0	0.0
30	0.18602294874607947	0.0	0.0	0.0	0.0
31	0.19433186623126864	0.0	0.0	0.0	0.0
32	0.2048188494650025	0.0	0.0	0.0	0.0
33	0.2098203337764756	0.0	0.0	0.0	0.0
34	0.21482181808794867	0.0	0.0	0.0	0.0
35	0.24434670934599945	0.0	0.0	0.0	0.0
36	0.2531396414419763	0.0	0.0	0.0	0.0
37	0.260641867909186	0.0	0.0	0.0	0.0
38	0.2671760651548201	0.0	0.0	0.0	0.0
39	0.27451695341843385	0.0	0.0	0.0	0.0
40	0.2829872091072189	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	35	1.0196527E-7	46.000004	6
GATGCGT	35	1.0196527E-7	46.000004	18
GTCATAC	30	1.8615228E-6	46.0	11
CTATGCG	20	6.311648E-4	46.0	30
ATCTCGT	20	6.311648E-4	46.0	10
ACACGTA	40	5.6115823E-9	46.0	14
ATTAGAC	20	6.311648E-4	46.0	38
AATCACG	20	6.311648E-4	46.0	22
CAACGTA	20	6.311648E-4	46.0	43
CGAAAAT	20	6.311648E-4	46.0	46
GACGTAT	20	6.311648E-4	46.0	10
CCAATTT	20	6.311648E-4	46.0	18
CCAATTA	30	1.8615228E-6	46.0	41
TTCACGG	125	0.0	46.0	29
GTGATAG	20	6.311648E-4	46.0	40
ATTCGAT	25	3.4173114E-5	46.0	28
CGATTCC	30	1.8615228E-6	46.0	12
TCGATAG	25	3.4173114E-5	46.0	43
TTATAGC	25	3.4173114E-5	46.0	36
AAACGTT	25	3.4173114E-5	46.0	39
>>END_MODULE