FastQCFastQC Report
Fri 17 Jun 2016
SRR1527049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196894
Sequences flagged as poor quality0
Sequence length52
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG53200.4444838055834518No Hit
GCCCTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT43330.36202036270546933No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA40880.34155071376412616No Hit
GCCCTCGGGATCAGTGGTAGCTTAACAACCTCTGGATTGATTGTGTATAATT35090.2931755025925437No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG34250.28615733724122605No Hit
GCCCTCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG31530.2634318494369593No Hit
GCCCTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC31140.2601734155238476No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATA27740.23176655576851415No Hit
GCCCTCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA27720.2315994565934828No Hit
GCCCTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC27530.23001201443068475No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT26280.21956831599122395No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC25310.21146400600220236No Hit
GCCCTCGGGCTCTTTCCCACAACTGTGCTGTGTCCTTGTTGCTCTGCTTCCT24070.20110385715025725No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC23750.1984302703497553No Hit
GCCCTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC21410.17887966687108464No Hit
GCCCTCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC20120.1681017700815611No Hit
GCCCTCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC19570.16350654276819837No Hit
GCCCTCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA19220.16058230720514935No Hit
GCCCTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18990.15866066669228854No Hit
GCCCTCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG18590.1553186831916611No Hit
GCCCTCGGAGCTGCCCTCGGGGGAGTGTGTGTGACTAACCCTTACTATTTAA18540.15490093525408263No Hit
GCCCTCGGGGTTGCACTGCGCTATTTCCCGCGCGGGCTAACCTCGTTCCCAG17410.1454598318648101No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17210.14378884011449636No Hit
GCCCTCGGGGTCCTAAATATCCAAAGACTGTACTGGCCAGTGTCCTTTACTT16530.13810746816342967No Hit
GCCCTCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG16510.13794036898839832No Hit
GCCCTCGGGCTCGCTACGTCCAACGCGCCATCAGCTGGAGGCGAGTAGATGC15980.13351224085006694No Hit
GCCCTCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT15660.13083865404956496No Hit
GCCCTCGGGATGTTGATAATGCTCGGGATATTTTTCAATGTCCATTCTGCTG15630.13058800528701792No Hit
GCCCTCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA15300.12783086889900025No Hit
GCCCTCGGGGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTCAAA15140.12649407549874928No Hit
GCCCTCGGGAGCCTCGCTTGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTG14660.12248369529799631No Hit
GCCCTCGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA14480.12097980272271396No Hit
GCCCTCGGGGGATATTTATGGCCTGAATACTGCTCTCCTACAGAGAATTCGC12860.10744476954517275No Hit
GCCCTCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA12750.1065257240825002No Hit
GCCCTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG12570.10502183150721785No Hit
GCCCTCGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC12010.10034305460633942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC206.3115585E-446.00000418
ACACGAA351.0196163E-746.00000420
CAACGTT405.6115823E-946.00000419
CCGATTT206.3115585E-446.00000443
TATGGTA206.3115585E-446.00000438
TTATGAC206.3115585E-446.00000433
ATTACGC206.3115585E-446.00000431
ATTCGGC206.3115585E-446.00000425
ATTCGAT206.3115585E-446.00000428
CGATTCC206.3115585E-446.00000412
TTATCGA405.6115823E-946.00000414
ACGATTA206.3115585E-446.00000440
ACGATAA206.3115585E-446.00000442
AATTGCG206.3115585E-446.00000420
TAATTCG206.3115585E-446.00000423
GGTATAC206.3115585E-446.0000049
CGAATGT206.3115585E-446.00000416
GCGATTA206.3115585E-446.00000430
CGTACTC206.3115585E-446.00000435
CGTACTA351.0196163E-746.00000428
Produced by FastQC (version 0.11.5)