FastQCFastQC Report
Fri 17 Jun 2016
SRR1527048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694812
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTG63580.9150676729820441No Hit
AAGCGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC35820.5155351375623909No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG29720.4277416049233462No Hit
AAGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC29340.42227249961140567No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA23260.33476681462035773No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC21070.3032474971647007No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA18040.2596385784931751No Hit
AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA17740.2553208637732221No Hit
AAGCGGGGGGACGCAGAGCCCTTGTACCCTTTATCTGGCTTTCCCCAGTG14380.20696245890974826No Hit
AAGCGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC14240.20494752537377017No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG13330.19185045738991266No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG12140.174723522334099No Hit
AAGCGGGGGACCCGGTTCCTTCTTTGCTTACAAAGGCTTGCTCCTTCCTC11290.1624899972942321No Hit
AAGCGGGGGGTCTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC11100.15975544463826186No Hit
AAGCGGGGGGAGGGTCTGTGGCTAGGTGCAGACTGGGTGCAGAACATCAC10980.15802835875028065No Hit
AAGCGGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG10670.1535667202063292No Hit
AAGCGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT9860.14190889046245603No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9700.1396061092784811No Hit
AAGCGGGGGGAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAAAA9590.13802294721449831No Hit
AAGCGGGGGAGTGATTTCACAGACACTGCCATGCAGAAACATAAGAAACA9370.13485662308653276No Hit
AAGCGGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG9330.13428092779053902No Hit
AAGCGGGGAGAGGAGCCCGAGGTCCGGGCCTTTGACCCAGACACAGCAGC9300.13384915631854372No Hit
AAGCGGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT9080.13068283219057816No Hit
AAGCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC8970.1290996701265954No Hit
AAGCGGGGGATCGTGTTGAGTGTAAGCTGTTGTAGTGATGTTAGCTATGT8880.1278043557106095No Hit
AAGCGGGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGT8760.1260772698226283No Hit
AAGCGGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCT8590.12363056481465491No Hit
AAGCGGGGGATCTGCCGCCGTAACCTAGATATTGAGCGTCCAACCTATAC8520.12262309804666587No Hit
AAGCGGGGGCAGTATTTTTTCCTGAAAGACTCTAGAGTCTGTGGGGAAAG8420.12118385980668153No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG8050.11585867831873946No Hit
AAGCGGGGGAGATGAGTATCTTGTGCGTGTTTAGGGGGTCCTGCCGTTAC8000.11513905919874728No Hit
AAGCGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT7980.11485121155075041No Hit
AAGCGGGGGATCGCTTTCTTCCTTCTCTGAAGTGTTAACACTTATGGAAG7980.11485121155075041No Hit
AAGCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGC7900.11369982095876295No Hit
AAGCGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC7880.11341197331076608No Hit
AAGCGGGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCC7630.10981387771080522No Hit
AAGCGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGG7200.10362515327887256No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG7140.10276161033488196No Hit
AAGCGGGGGATAATCTTAGAGGCAAACTTTGAGACAGGTGTTTTGAGGCT7030.10117844827089918No Hit
AAGCGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT6950.10002705767891171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT207.8543293E-444.016
GTCATAT207.8543293E-444.024
AACGTCC207.8543293E-444.014
AACCGTT302.5262652E-644.043
CGGGTAT207.8543293E-444.037
TAATAAC254.4410175E-544.020
TGATACG302.5262652E-644.037
GATACCC207.8543293E-444.014
CTCACGA454.783942E-1044.015
TCGTTAT207.8543293E-444.039
AAATCGC207.8543293E-444.036
CAAACGT207.8543293E-444.025
GCCCAAT207.8543293E-444.016
CTCGTTA207.8543293E-444.016
CGTATTG551.8189894E-1244.012
CATATGC207.8543293E-444.023
ATCTATT207.8543293E-444.010
GATGTAG207.8543293E-444.014
CGTATGA254.4410175E-544.020
AGTTTCG207.8543293E-444.013
Produced by FastQC (version 0.11.5)