##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	694812
Sequences flagged as poor quality	0
Sequence length	50
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36855293230399	33.0	31.0	34.0	31.0	34.0
2	32.95906950369309	34.0	31.0	34.0	31.0	34.0
3	32.959004737972286	34.0	31.0	34.0	31.0	34.0
4	36.27808673425329	37.0	37.0	37.0	35.0	37.0
5	36.28791817067063	37.0	37.0	37.0	35.0	37.0
6	36.32174026931026	37.0	37.0	37.0	35.0	37.0
7	36.61165466341974	37.0	37.0	37.0	35.0	37.0
8	36.655056331784714	37.0	37.0	37.0	35.0	37.0
9	38.60204199121489	39.0	39.0	39.0	38.0	39.0
10	35.76833301670092	35.0	35.0	39.0	33.0	39.0
11	35.03378611768363	35.0	35.0	39.0	31.0	39.0
12	34.78680276103464	35.0	34.0	39.0	28.0	39.0
13	34.98269172092595	35.0	34.0	39.0	30.0	39.0
14	35.644444828241305	35.0	35.0	40.0	31.0	40.0
15	35.776290564929795	35.0	35.0	40.0	32.0	40.0
16	35.71518050926006	35.0	35.0	40.0	32.0	40.0
17	35.756262125582175	35.0	35.0	40.0	32.0	40.0
18	35.73587531591279	35.0	35.0	40.0	32.0	40.0
19	35.627161016217336	35.0	35.0	40.0	32.0	40.0
20	35.29031882005492	35.0	34.0	40.0	31.0	40.0
21	35.422163117505164	35.0	35.0	40.0	31.0	40.0
22	35.156047966932064	35.0	34.0	39.0	31.0	40.0
23	35.00613547261705	35.0	34.0	39.0	31.0	40.0
24	35.029101397212486	35.0	34.0	39.0	31.0	40.0
25	34.906672596328214	35.0	34.0	39.0	31.0	40.0
26	34.69254129174511	35.0	34.0	38.0	30.0	40.0
27	34.50732716187976	35.0	34.0	38.0	30.0	40.0
28	34.238641531810046	35.0	34.0	37.0	29.0	40.0
29	34.398519311698706	35.0	34.0	37.0	30.0	40.0
30	34.42790855655918	35.0	34.0	37.0	30.0	39.0
31	34.293938504228485	35.0	34.0	36.0	30.0	39.0
32	33.62217405571579	35.0	34.0	36.0	28.0	39.0
33	33.68230830785882	35.0	33.0	36.0	28.0	39.0
34	33.66753020961066	35.0	33.0	35.0	29.0	39.0
35	33.63298849185103	35.0	34.0	35.0	29.0	37.0
36	33.485629206173755	35.0	33.0	35.0	29.0	37.0
37	33.432362998911934	35.0	33.0	35.0	29.0	37.0
38	33.236312844337746	35.0	33.0	35.0	27.0	37.0
39	33.22722837256697	35.0	33.0	35.0	27.0	37.0
40	33.07835356902299	35.0	33.0	35.0	27.0	37.0
41	32.95638964209024	35.0	33.0	35.0	27.0	36.0
42	32.897634180181115	35.0	33.0	35.0	27.0	36.0
43	32.9005673477142	35.0	33.0	35.0	27.0	36.0
44	32.788010281917984	35.0	33.0	35.0	27.0	36.0
45	32.7938492714576	35.0	33.0	35.0	27.0	36.0
46	32.573378122427364	35.0	33.0	35.0	25.0	36.0
47	32.53564561348969	35.0	33.0	35.0	24.0	35.0
48	32.458149830457735	35.0	33.0	35.0	24.0	35.0
49	32.29246184579426	35.0	33.0	35.0	24.0	35.0
50	31.859501563012728	35.0	32.0	35.0	23.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	11.0
15	32.0
16	113.0
17	218.0
18	430.0
19	731.0
20	1040.0
21	1765.0
22	2484.0
23	3478.0
24	4539.0
25	5916.0
26	7330.0
27	9134.0
28	11689.0
29	14721.0
30	19716.0
31	25425.0
32	35291.0
33	59031.0
34	153515.0
35	126516.0
36	95387.0
37	84392.0
38	26848.0
39	5056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.64567681617473	1.1582989355393976	0.09139162823900567	0.10463262004686158
8	98.66424298947052	1.0136554924209715	0.22005952689360575	0.1020419912148898
9	93.16405013154638	4.67651681318112	1.3104264175057425	0.8490066377667628
10	55.799410488016896	30.14599632706401	5.487815409060293	8.566777775858792
11	34.271716665803126	22.16657167694283	21.578355008261227	21.98335664899282
12	26.361807222673185	16.252022129727177	29.296413994001252	28.089756653598386
13	24.80498321848212	15.279097079497763	30.58539576173123	29.330523940288884
14	24.16812029728905	17.09383257629402	32.42215160359925	26.315895522817684
15	25.799065070839305	17.936506565804848	28.605435714984772	27.658992648371072
16	27.993615539167426	17.383695157826864	24.655158517699753	29.96753078530595
17	28.58658169404098	19.2555684127505	22.933397811206486	29.22445208200204
18	28.738853099831317	17.719613363039212	24.10911152944969	29.432422007679776
19	29.616788426221767	17.598861274704525	21.714363021939747	31.069987277133958
20	29.654064696637363	18.502127194118696	24.777781615746417	27.066026493497517
21	32.93509611232967	16.138178384944414	24.267139888199974	26.659585614525945
22	32.60766941273323	16.777056239673467	22.51314024513106	28.10213410246225
23	28.117246103982087	19.567595263179104	25.055554596063395	27.259604036775414
24	28.832979280726295	20.14818396918879	23.598181954255253	27.420654795829662
25	27.098697201545168	19.827521689320278	23.989079060235	29.08470204889956
26	28.96682843704484	20.05794373154177	23.826589062940766	27.148638768472622
27	31.633592971911828	17.316914503491592	22.785444120136095	28.264048404460485
28	29.968250404425945	19.843929005256097	21.71637795547573	28.47144263484223
29	29.8345451719314	18.480106849046937	21.912977899057587	29.772370079964077
30	28.70718985855167	18.854164867618866	22.759681755640372	29.678963518189093
31	31.494706481753337	19.530750764235506	22.286460222333524	26.688082531677633
32	33.409756883876504	19.19857457844712	21.498621209766096	25.893047327910285
33	30.906633736895735	20.245332550387733	22.422612159836042	26.42542155288049
34	29.803745473595733	21.40550249563911	24.663937870963657	24.126814159801498
35	32.23274785121731	21.96608579011301	21.63318998520463	24.167976373465052
36	33.22510261768651	21.383338226743348	19.34206663097356	26.04949252459658
37	30.501344248516144	21.844182311186337	20.82534556110142	26.829127879196097
38	31.871642976805237	20.76115553559812	22.259402543421817	25.107798944174824
39	30.682976114402173	20.847653753821177	21.721271365491674	26.748098766284983
40	29.512587577646904	21.898297669009747	22.61201591221798	25.97709884112537
41	28.165460585021563	20.614209311295717	22.64238383908165	28.577946264601074
42	28.766198626391027	19.979217399814626	24.91983443003287	26.334749543761475
43	30.817688813664702	18.469456486071053	24.619897180820136	26.09295751944411
44	26.825098012124144	18.563870514614024	25.703499651704348	28.907531821557487
45	28.060108345854708	18.729095064564227	25.91809007328601	27.292706516295056
46	30.79509277329695	20.21395715675607	22.67390315653731	26.31704691340967
47	28.252678422364614	20.196542374052264	25.359665636172085	26.19111356741104
48	28.41847866761081	20.895868234860654	24.518574808725237	26.167078288803303
49	27.387408392486023	21.32188275389602	25.021588573599768	26.269120280018193
50	27.784494222897706	21.79783883985884	22.552575372906627	27.86509156433683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	30.0
14	58.0
15	160.5
16	263.0
17	607.5
18	952.0
19	698.0
20	444.0
21	671.5
22	899.0
23	1100.5
24	1302.0
25	1119.5
26	937.0
27	1287.0
28	1637.0
29	2533.0
30	3429.0
31	3155.0
32	2881.0
33	3170.5
34	3460.0
35	3790.0
36	4120.0
37	5344.5
38	6569.0
39	11135.0
40	15701.0
41	13168.0
42	10635.0
43	10243.0
44	9851.0
45	13248.5
46	16646.0
47	16604.0
48	16562.0
49	18614.0
50	20666.0
51	21994.5
52	23323.0
53	26548.0
54	29773.0
55	34454.0
56	39135.0
57	41356.5
58	43578.0
59	50590.0
60	57602.0
61	63305.5
62	69009.0
63	70694.0
64	72379.0
65	64701.0
66	57023.0
67	55288.0
68	53553.0
69	47545.5
70	41538.0
71	34544.5
72	27551.0
73	24894.5
74	22238.0
75	18246.0
76	14254.0
77	16296.0
78	18338.0
79	12139.5
80	5941.0
81	3731.5
82	1522.0
83	1126.5
84	731.0
85	466.5
86	202.0
87	141.5
88	81.0
89	51.5
90	22.0
91	13.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	694812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.712825915499446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2805975763764	7.636166329884919
2	11.205900529327996	2.4009372319418776
3	4.180885079399199	1.3436728208493809
4	2.1522422548835207	0.9222638641819658
5	1.150012091248623	0.615993966713298
6	0.7308488056533305	0.46976736153088894
7	0.5252975790633313	0.39391950628371414
8	0.3251202407501948	0.27863652326096844
9	0.22435983555901873	0.21631750746964645
>10	3.154472418518419	9.196300582027945
>50	2.5794663728941076	20.175961267220487
>100	2.3671977859580298	44.18763636782324
>500	0.10210387726039176	7.316511516784397
>1k	0.020152081038235216	3.9308474810452325
>5k	0.001343472069215681	0.9150676729820441
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCGGGGGAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTG	6358	0.9150676729820441	No Hit
AAGCGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3582	0.5155351375623909	No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2972	0.4277416049233462	No Hit
AAGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2934	0.42227249961140567	No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2326	0.33476681462035773	No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2107	0.3032474971647007	No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1804	0.2596385784931751	No Hit
AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1774	0.2553208637732221	No Hit
AAGCGGGGGGACGCAGAGCCCTTGTACCCTTTATCTGGCTTTCCCCAGTG	1438	0.20696245890974826	No Hit
AAGCGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1424	0.20494752537377017	No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1333	0.19185045738991266	No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1214	0.174723522334099	No Hit
AAGCGGGGGACCCGGTTCCTTCTTTGCTTACAAAGGCTTGCTCCTTCCTC	1129	0.1624899972942321	No Hit
AAGCGGGGGGTCTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC	1110	0.15975544463826186	No Hit
AAGCGGGGGGAGGGTCTGTGGCTAGGTGCAGACTGGGTGCAGAACATCAC	1098	0.15802835875028065	No Hit
AAGCGGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	1067	0.1535667202063292	No Hit
AAGCGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT	986	0.14190889046245603	No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	970	0.1396061092784811	No Hit
AAGCGGGGGGAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAAAA	959	0.13802294721449831	No Hit
AAGCGGGGGAGTGATTTCACAGACACTGCCATGCAGAAACATAAGAAACA	937	0.13485662308653276	No Hit
AAGCGGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	933	0.13428092779053902	No Hit
AAGCGGGGAGAGGAGCCCGAGGTCCGGGCCTTTGACCCAGACACAGCAGC	930	0.13384915631854372	No Hit
AAGCGGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	908	0.13068283219057816	No Hit
AAGCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	897	0.1290996701265954	No Hit
AAGCGGGGGATCGTGTTGAGTGTAAGCTGTTGTAGTGATGTTAGCTATGT	888	0.1278043557106095	No Hit
AAGCGGGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGT	876	0.1260772698226283	No Hit
AAGCGGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCT	859	0.12363056481465491	No Hit
AAGCGGGGGATCTGCCGCCGTAACCTAGATATTGAGCGTCCAACCTATAC	852	0.12262309804666587	No Hit
AAGCGGGGGCAGTATTTTTTCCTGAAAGACTCTAGAGTCTGTGGGGAAAG	842	0.12118385980668153	No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	805	0.11585867831873946	No Hit
AAGCGGGGGAGATGAGTATCTTGTGCGTGTTTAGGGGGTCCTGCCGTTAC	800	0.11513905919874728	No Hit
AAGCGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	798	0.11485121155075041	No Hit
AAGCGGGGGATCGCTTTCTTCCTTCTCTGAAGTGTTAACACTTATGGAAG	798	0.11485121155075041	No Hit
AAGCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGC	790	0.11369982095876295	No Hit
AAGCGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC	788	0.11341197331076608	No Hit
AAGCGGGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCC	763	0.10981387771080522	No Hit
AAGCGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGG	720	0.10362515327887256	No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG	714	0.10276161033488196	No Hit
AAGCGGGGGATAATCTTAGAGGCAAACTTTGAGACAGGTGTTTTGAGGCT	703	0.10117844827089918	No Hit
AAGCGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	695	0.10002705767891171	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.756952959937364E-4	0.0	0.0	0.0	0.0
8	8.635429439906047E-4	0.0	0.0	0.0	0.0
9	0.001295314415985907	0.0	0.0	0.0	0.0
10	0.005037333839945194	0.0	0.0	0.0	0.0
11	0.011226058271877861	0.0	0.0	0.0	0.0
12	0.01367276327985124	0.0	0.0	0.0	0.0
13	0.019573640063787038	0.0	0.0	0.0	0.0
14	0.02547451684772284	0.0	0.0	0.0	0.0
15	0.03223893657564924	0.0	0.0	0.0	0.0
16	0.03885943247957721	0.0	0.0	0.0	0.0
17	0.043033223375531796	0.0	0.0	0.0	0.0
18	0.047350938095484824	0.0	0.0	0.0	0.0
19	0.05138080516744098	0.0	0.0	0.0	0.0
20	0.054403205471408096	0.0	0.0	0.0	0.0
21	0.058289148719365816	0.0	0.0	0.0	0.0
22	0.0628947110873157	0.0	0.0	0.0	0.0
23	0.06548533991928752	0.0	0.0	0.0	0.0
24	0.07037874993523428	0.0	0.0	0.0	0.0
25	0.07354507406319984	0.0	0.0	0.0	0.0
26	0.07685532201516382	0.0	0.0	0.0	0.0
27	0.0810291129111184	0.0	0.0	0.0	0.0
28	0.08534682763107143	0.0	0.0	0.0	0.0
29	0.09124770441500722	0.0	0.0	0.0	0.0
30	0.09657288590294928	0.0	0.0	0.0	0.0
31	0.10204199121488979	0.0	0.0	0.0	0.0
32	0.10664755358283967	0.0	0.0	0.0	0.0
33	0.11110919212679113	0.0	0.0	0.0	0.0
34	0.11816145950271441	0.0	0.0	0.0	0.0
35	0.1273725842386142	0.0	0.0	0.0	0.0
36	0.13068283219057816	0.0	0.0	0.0	0.0
37	0.13572016603052336	0.0	0.0	0.0	0.0
38	0.14147711899046073	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTT	20	7.8543293E-4	44.0	16
GTCATAT	20	7.8543293E-4	44.0	24
AACGTCC	20	7.8543293E-4	44.0	14
AACCGTT	30	2.5262652E-6	44.0	43
CGGGTAT	20	7.8543293E-4	44.0	37
TAATAAC	25	4.4410175E-5	44.0	20
TGATACG	30	2.5262652E-6	44.0	37
GATACCC	20	7.8543293E-4	44.0	14
CTCACGA	45	4.783942E-10	44.0	15
TCGTTAT	20	7.8543293E-4	44.0	39
AAATCGC	20	7.8543293E-4	44.0	36
CAAACGT	20	7.8543293E-4	44.0	25
GCCCAAT	20	7.8543293E-4	44.0	16
CTCGTTA	20	7.8543293E-4	44.0	16
CGTATTG	55	1.8189894E-12	44.0	12
CATATGC	20	7.8543293E-4	44.0	23
ATCTATT	20	7.8543293E-4	44.0	10
GATGTAG	20	7.8543293E-4	44.0	14
CGTATGA	25	4.4410175E-5	44.0	20
AGTTTCG	20	7.8543293E-4	44.0	13
>>END_MODULE