FastQCFastQC Report
Fri 17 Jun 2016
SRR1527047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742379
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGAT60910.8204704066251874No Hit
AAGCGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32570.43872469452934415No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG28920.38955843309145327No Hit
AAGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC28570.384843860076861No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG22990.309680096015647No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC20660.27829450994707555No Hit
AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA17300.2330346090069897No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16890.22751182347561016No Hit
AAGCGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG13300.17915377455450654No Hit
AAGCGGGGGGACGCAGAGCCCTTGTACCCTTTATCTGGCTTTCCCCAGTGCT13070.17605562657348875No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13000.1751127119705703No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10980.1479028905720663No Hit
AAGCGGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT10160.13685731950930724No Hit
AAGCGGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT10160.13685731950930724No Hit
AAGCGGGGGACCCGGTTCCTTCTTTGCTTACAAAGGCTTGCTCCTTCCTCCA10040.13524089447573276No Hit
AAGCGGGGGGTCTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG9750.13133453397792771No Hit
AAGCGGGGGGAGGGTCTGTGGCTAGGTGCAGACTGGGTGCAGAACATCACAA9230.12433002549910491No Hit
AAGCGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC9080.12230949420713678No Hit
AAGCGGGGGATCTGCCGCCGTAACCTAGATATTGAGCGTCCAACCTATACCA9070.12217479212100557No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG9060.12204009003487437No Hit
AAGCGGGGGGAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAAAAAC8640.11638260241736363No Hit
AAGCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8610.11597849615897No Hit
AAGCGGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8470.1140926669531331No Hit
AAGCGGGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGC8450.11382326278087068No Hit
AAGCGGGGAGAGGAGCCCGAGGTCCGGGCCTTTGACCCAGACACAGCAGCCG8320.11207213566116497No Hit
AAGCGGGGGATCGTGTTGAGTGTAAGCTGTTGTAGTGATGTTAGCTATGTAA8310.11193743357503377No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG7740.10425941466555493No Hit
AAGCGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC7740.10425941466555493No Hit
AAGCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGT7690.10358590423489887No Hit
AAGCGGGGGATCGCTTTCTTCCTTCTCTGAAGTGTTAACACTTATGGAAGGA7520.10129596877066836No Hit
AAGCGGGGGAGTGTGAATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGAT7460.10048775625388111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTGG1050.046.00000432
GCATAAA206.3100026E-446.039
GTGCATA206.3100026E-446.037
CTAGACC253.415979E-546.027
AACCGAA253.415979E-546.023
ACACGTA206.3100026E-446.034
GCCCAAT206.3100026E-446.016
GTATTCG206.3100026E-446.027
TTAATAG253.415979E-546.019
CTCAACG501.6370905E-1146.019
TACCAAG206.3100026E-446.031
CCGATAC206.3100026E-446.018
CGTGCAA253.415979E-546.018
TAGGTTA253.415979E-546.018
ACCGCGA206.3100026E-446.026
CGTTGTC600.046.041
TAGCGTA206.3100026E-446.042
GACGTAA253.415979E-546.023
ATTACAT206.3100026E-446.019
CACGCTA253.415979E-546.027
Produced by FastQC (version 0.11.5)