##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742379
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.287521602847065	33.0	31.0	34.0	31.0	34.0
2	32.89483404029478	34.0	31.0	34.0	31.0	34.0
3	32.86417584549132	34.0	31.0	34.0	31.0	34.0
4	36.21552199078907	37.0	37.0	37.0	35.0	37.0
5	36.25351875524496	37.0	37.0	37.0	35.0	37.0
6	36.32930618996497	37.0	37.0	37.0	35.0	37.0
7	36.61252406116014	37.0	37.0	37.0	35.0	37.0
8	36.61436813271927	37.0	37.0	37.0	35.0	37.0
9	38.56150025795449	39.0	39.0	39.0	37.0	39.0
10	35.65703771254305	35.0	35.0	39.0	33.0	39.0
11	34.959341522322156	35.0	35.0	39.0	30.0	39.0
12	34.72424327735563	35.0	33.0	39.0	27.0	39.0
13	34.98296692120871	35.0	34.0	39.0	30.0	39.0
14	35.63873304605868	35.0	35.0	40.0	31.0	40.0
15	35.663277113172654	35.0	35.0	40.0	32.0	40.0
16	30.89170086977137	35.0	32.0	40.0	3.0	40.0
17	31.933396553512424	35.0	32.0	40.0	12.0	40.0
18	33.85428871236929	35.0	33.0	40.0	23.0	40.0
19	34.945894213063674	35.0	34.0	40.0	30.0	40.0
20	35.28794995548096	35.0	34.0	39.0	31.0	40.0
21	35.508208071618405	35.0	35.0	40.0	31.0	40.0
22	35.44250712910791	35.0	35.0	39.0	31.0	40.0
23	35.21702930713288	35.0	34.0	39.0	31.0	40.0
24	35.16713430740902	35.0	34.0	39.0	31.0	40.0
25	35.03407019864517	35.0	34.0	39.0	31.0	40.0
26	34.84478548019273	35.0	34.0	38.0	31.0	40.0
27	34.6511902949841	35.0	34.0	37.0	30.0	40.0
28	34.43227381162452	35.0	34.0	37.0	30.0	40.0
29	34.178722727878885	35.0	34.0	37.0	30.0	39.0
30	34.128754989028515	35.0	34.0	37.0	29.0	39.0
31	33.979195262797035	35.0	33.0	36.0	29.0	39.0
32	33.93721266361252	35.0	33.0	36.0	30.0	39.0
33	33.508358937954874	35.0	33.0	35.0	28.0	39.0
34	33.44322239718527	35.0	33.0	35.0	28.0	38.0
35	33.31115373683792	35.0	33.0	35.0	27.0	37.0
36	33.2793276749477	35.0	33.0	35.0	28.0	37.0
37	32.9546673599334	35.0	33.0	35.0	26.0	37.0
38	32.94895599148144	35.0	33.0	35.0	26.0	37.0
39	32.617114708255485	35.0	33.0	35.0	25.0	36.0
40	32.543277759742665	35.0	33.0	35.0	24.0	36.0
41	32.49884762365315	35.0	33.0	35.0	24.0	36.0
42	32.47437494864483	35.0	33.0	35.0	25.0	36.0
43	32.496867502987016	35.0	33.0	35.0	25.0	36.0
44	32.819325438893074	35.0	33.0	35.0	27.0	36.0
45	33.02425176358706	35.0	33.0	35.0	28.0	36.0
46	33.03046152975771	35.0	33.0	35.0	29.0	35.0
47	32.81160027425345	35.0	33.0	35.0	27.0	35.0
48	32.79129797583175	35.0	33.0	35.0	27.0	35.0
49	32.842471298353	35.0	33.0	35.0	27.0	35.0
50	32.40545058521322	35.0	33.0	35.0	25.0	35.0
51	32.542808996482925	35.0	33.0	35.0	26.0	35.0
52	32.225198988656736	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	3.0
14	11.0
15	23.0
16	77.0
17	213.0
18	400.0
19	720.0
20	1192.0
21	1749.0
22	2694.0
23	3747.0
24	4990.0
25	6595.0
26	8428.0
27	10611.0
28	13666.0
29	17629.0
30	23159.0
31	32819.0
32	50941.0
33	91006.0
34	162053.0
35	123904.0
36	94509.0
37	68890.0
38	18927.0
39	3422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.7352821133141	1.1282646734349975	0.06667753263494793	0.0697756806159657
8	98.68476883101489	1.0059551792278607	0.20986585019242193	0.09941013956483145
9	93.21977049458565	4.632135337879978	1.3137494460376709	0.8343447214967018
10	56.003335223652606	29.95289468047992	5.430918708638041	8.612851387229433
11	34.28370145168439	22.17492682309171	21.56041590616114	21.98095581906277
12	26.416830217449576	16.263121666965255	29.386472408298186	27.933575707286977
13	25.037211451293746	15.183888552882019	30.434589340485118	29.344310655339118
14	24.20690779238098	16.944310116530776	32.397333437502944	26.451448653585295
15	25.833435482415318	17.924402495221443	28.54808662421755	27.69407539814569
16	26.864849355921976	25.435525519983727	21.808671850900954	25.89095327319334
17	28.617862304833512	19.1629881771979	22.890329602534553	29.32881991543403
18	28.928754719624344	17.60960371993281	24.16528484776644	29.296356712676406
19	29.759462484795502	17.577409921347453	21.538594168207883	31.124533425649165
20	29.688339783318224	18.463076137660146	24.769019597806512	27.07956448121512
21	33.119336619166226	15.961388994031351	24.255400543388212	26.663873843414216
22	32.344799623911776	16.71854942017487	22.68019434816987	28.256456607743484
23	28.20338398580779	19.54904435604994	24.828692621962638	27.418879036179632
24	28.897369133555774	19.966890227228948	23.55441088716141	27.58132975205387
25	27.092495881483718	19.667582191845405	23.95919065598569	29.28073127068519
26	29.077465822713194	19.925401984700535	24.016843148849844	26.980289043736423
27	31.722071879727203	17.208595609520206	22.70026495900342	28.369067551749172
28	29.965826080748514	19.950995381065468	21.64891517674934	28.434263361436678
29	30.180271801869395	18.40475013436533	21.8079983404703	29.606979723294973
30	28.692352558464073	18.749991581119616	22.744312541168323	29.813343319247988
31	31.567433884848572	19.603733403019213	22.149872235071307	26.678960477060908
32	33.535835469483914	18.747566943569254	21.52040938658017	26.19618820036666
33	31.10109526266233	20.059026454142696	22.459821735259215	26.38005654793576
34	29.92972592166535	21.29747743403302	24.7253761219	24.04742052240163
35	32.31947563171911	21.806651319608985	21.662250683276334	24.211622365395574
36	33.22130609836755	21.22904877427837	19.329749359828334	26.219895767525752
37	30.68958038953149	21.939198172362097	20.695089704854258	26.676131733252152
38	31.85798628463359	20.717854357410435	22.123066519931196	25.30109283802478
39	30.895674581312242	20.81793800740592	21.613892634355228	26.67249477692661
40	29.683894614475893	21.97422071475621	22.516261909348188	25.82562276141971
41	28.216180683990256	20.547186814282192	22.540508284851807	28.696124216875745
42	28.7921668042873	19.953824124874224	24.79434358999918	26.459665480839302
43	30.898638027207127	18.272472685784486	24.59727443798922	26.23161484901917
44	26.820262965412546	18.57784231504393	25.65104885779366	28.950845861749862
45	27.93263279268406	18.7848794214276	26.00545004640487	27.27703773948347
46	30.654153740878986	20.105902780116356	22.63911021189985	26.600833267104807
47	28.39977962738709	20.19561436947974	25.23118245532269	26.173423547810486
48	28.698548854426107	20.725532376319915	24.370032018685873	26.20588675056811
49	27.379007218684798	21.205880015463798	25.08678181899003	26.32833094686137
50	27.690707845992414	21.86955719383226	22.59869958606049	27.841035374114835
51	27.185844427172647	23.126866465780953	21.97556773561752	27.711721371428876
52	28.035949292746697	21.636926691083666	23.953398466282046	26.373725549887588
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	4.5
12	7.0
13	28.5
14	163.0
15	276.0
16	597.0
17	918.0
18	685.0
19	452.0
20	697.5
21	943.0
22	1102.0
23	1261.0
24	1042.0
25	823.0
26	1200.0
27	1577.0
28	1825.5
29	2074.0
30	2722.0
31	3370.0
32	2990.0
33	2610.0
34	3199.5
35	3789.0
36	4726.5
37	5664.0
38	10060.0
39	12037.0
40	9618.0
41	10219.5
42	10821.0
43	11562.0
44	12303.0
45	13318.5
46	14334.0
47	15761.0
48	17188.0
49	19504.0
50	21820.0
51	23697.0
52	25574.0
53	28921.0
54	32268.0
55	34385.0
56	36502.0
57	40641.0
58	44780.0
59	52692.5
60	60605.0
61	69057.0
62	77509.0
63	72538.5
64	65641.0
65	63714.0
66	59012.0
67	54310.0
68	51213.0
69	48116.0
70	40195.0
71	32274.0
72	28029.5
73	23785.0
74	21245.5
75	18706.0
76	19328.0
77	19950.0
78	14418.0
79	8886.0
80	5609.0
81	2332.0
82	1620.5
83	909.0
84	536.0
85	163.0
86	102.0
87	41.0
88	27.0
89	9.5
90	6.0
91	6.5
92	7.0
93	4.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	742379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.454285479519221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1038092971944	8.606385687095136
2	10.77570356269874	2.684073768250449
3	4.360899002790456	1.6293564338430908
4	2.3924376473642086	1.1918440580889276
5	1.5325877695818642	0.954364280239608
6	1.0750827402712584	0.803363241686524
7	0.7960371195569881	0.6939851477479833
8	0.5808042570680741	0.5786801620196692
9	0.5288887927491401	0.5928238810634461
>10	4.787038439075039	13.848452070977224
>50	2.16314434662225	19.547158526844104
>100	1.8224491120292456	39.34149538173898
>500	0.0648943303986675	5.358718390471713
>1k	0.015142010426355752	3.3488285633079604
>5k	0.001081572173311125	0.8204704066251874
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCGGGGGAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGAT	6091	0.8204704066251874	No Hit
AAGCGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3257	0.43872469452934415	No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2892	0.38955843309145327	No Hit
AAGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2857	0.384843860076861	No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2299	0.309680096015647	No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2066	0.27829450994707555	No Hit
AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1730	0.2330346090069897	No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1689	0.22751182347561016	No Hit
AAGCGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1330	0.17915377455450654	No Hit
AAGCGGGGGGACGCAGAGCCCTTGTACCCTTTATCTGGCTTTCCCCAGTGCT	1307	0.17605562657348875	No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1300	0.1751127119705703	No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1098	0.1479028905720663	No Hit
AAGCGGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1016	0.13685731950930724	No Hit
AAGCGGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT	1016	0.13685731950930724	No Hit
AAGCGGGGGACCCGGTTCCTTCTTTGCTTACAAAGGCTTGCTCCTTCCTCCA	1004	0.13524089447573276	No Hit
AAGCGGGGGGTCTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG	975	0.13133453397792771	No Hit
AAGCGGGGGGAGGGTCTGTGGCTAGGTGCAGACTGGGTGCAGAACATCACAA	923	0.12433002549910491	No Hit
AAGCGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC	908	0.12230949420713678	No Hit
AAGCGGGGGATCTGCCGCCGTAACCTAGATATTGAGCGTCCAACCTATACCA	907	0.12217479212100557	No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	906	0.12204009003487437	No Hit
AAGCGGGGGGAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAAAAAC	864	0.11638260241736363	No Hit
AAGCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	861	0.11597849615897	No Hit
AAGCGGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	847	0.1140926669531331	No Hit
AAGCGGGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGC	845	0.11382326278087068	No Hit
AAGCGGGGAGAGGAGCCCGAGGTCCGGGCCTTTGACCCAGACACAGCAGCCG	832	0.11207213566116497	No Hit
AAGCGGGGGATCGTGTTGAGTGTAAGCTGTTGTAGTGATGTTAGCTATGTAA	831	0.11193743357503377	No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	774	0.10425941466555493	No Hit
AAGCGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	774	0.10425941466555493	No Hit
AAGCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGT	769	0.10358590423489887	No Hit
AAGCGGGGGATCGCTTTCTTCCTTCTCTGAAGTGTTAACACTTATGGAAGGA	752	0.10129596877066836	No Hit
AAGCGGGGGAGTGTGAATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGAT	746	0.10048775625388111	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.694041722624158E-4	0.0	0.0	0.0	0.0
8	2.694041722624158E-4	0.0	0.0	0.0	0.0
9	8.082125167872475E-4	0.0	0.0	0.0	0.0
10	0.0036369563255426135	0.0	0.0	0.0	0.0
11	0.007543316823347643	0.0	0.0	0.0	0.0
12	0.009967954373709386	0.0	0.0	0.0	0.0
13	0.013874314871514415	0.0	0.0	0.0	0.0
14	0.018588887886106693	0.0	0.0	0.0	0.0
15	0.024785183848142257	0.0	0.0	0.0	0.0
16	0.033271415274408356	0.0	0.0	0.0	0.0
17	0.03812069037513184	0.0	0.0	0.0	0.0
18	0.04243115713133049	0.0	0.0	0.0	0.0
19	0.04633751762913552	0.0	0.0	0.0	0.0
20	0.04957036769628451	0.0	0.0	0.0	0.0
21	0.05401553653861437	0.0	0.0	0.0	0.0
22	0.05657487617510732	0.0	0.0	0.0	0.0
23	0.059942428328387524	0.0	0.0	0.0	0.0
24	0.06317527839553651	0.0	0.0	0.0	0.0
25	0.06559991594589826	0.0	0.0	0.0	0.0
26	0.06869806392691603	0.0	0.0	0.0	0.0
27	0.0732779348553771	0.0	0.0	0.0	0.0
28	0.07745369952544455	0.0	0.0	0.0	0.0
29	0.08230297462616804	0.0	0.0	0.0	0.0
30	0.08620933512397307	0.0	0.0	0.0	0.0
31	0.09321384360279587	0.0	0.0	0.0	0.0
32	0.09846722496191299	0.0	0.0	0.0	0.0
33	0.10291239380424284	0.0	0.0	0.0	0.0
34	0.10964749811080324	0.0	0.0	0.0	0.0
35	0.11799902745093813	0.0	0.0	0.0	0.0
36	0.12217479212100558	0.0	0.0	0.0	0.0
37	0.12890989642756598	0.0	0.0	0.0	0.0
38	0.132816256925371	0.0	0.0	0.0	0.0
39	0.13820434037061932	0.0	0.0	0.0	0.0
40	0.14332301964360522	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTGG	105	0.0	46.000004	32
GCATAAA	20	6.3100026E-4	46.0	39
GTGCATA	20	6.3100026E-4	46.0	37
CTAGACC	25	3.415979E-5	46.0	27
AACCGAA	25	3.415979E-5	46.0	23
ACACGTA	20	6.3100026E-4	46.0	34
GCCCAAT	20	6.3100026E-4	46.0	16
GTATTCG	20	6.3100026E-4	46.0	27
TTAATAG	25	3.415979E-5	46.0	19
CTCAACG	50	1.6370905E-11	46.0	19
TACCAAG	20	6.3100026E-4	46.0	31
CCGATAC	20	6.3100026E-4	46.0	18
CGTGCAA	25	3.415979E-5	46.0	18
TAGGTTA	25	3.415979E-5	46.0	18
ACCGCGA	20	6.3100026E-4	46.0	26
CGTTGTC	60	0.0	46.0	41
TAGCGTA	20	6.3100026E-4	46.0	42
GACGTAA	25	3.415979E-5	46.0	23
ATTACAT	20	6.3100026E-4	46.0	19
CACGCTA	25	3.415979E-5	46.0	27
>>END_MODULE