FastQCFastQC Report
Fri 17 Jun 2016
SRR1527046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723339
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGAT58870.8138645918442113No Hit
AAGCGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT33880.46838342741093736No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG29040.40147150920937485No Hit
AAGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC28240.3904116880190339No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG21630.2990299154338422No Hit
AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19900.27511305210973No Hit
AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA17390.24041286312503543No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16770.23184150170252124No Hit
AAGCGGGGGGACGCAGAGCCCTTGTACCCTTTATCTGGCTTTCCCCAGTGCT13510.18677273035188202No Hit
AAGCGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG12990.17958384657816043No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC12670.17515991810202408No Hit
AAGCGGGGGACCCGGTTCCTTCTTTGCTTACAAAGGCTTGCTCCTTCCTCCA10630.14695737406665477No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10560.14598963971249995No Hit
AAGCGGGGGGTCTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG10540.14571314418274142No Hit
AAGCGGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT9580.13244135875433236No Hit
AAGCGGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT9170.12677320039428264No Hit
AAGCGGGGGATCGTGTTGAGTGTAAGCTGTTGTAGTGATGTTAGCTATGTAA9020.1246994839210937No Hit
AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG8990.12428474062645592No Hit
AAGCGGGGAGAGGAGCCCGAGGTCCGGGCCTTTGACCCAGACACAGCAGCCG8960.12386999733181815No Hit
AAGCGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC8950.12373174956693887No Hit
AAGCGGGGGGAGGGTCTGTGGCTAGGTGCAGACTGGGTGCAGAACATCACAA8790.1215197853288707No Hit
AAGCGGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8400.11612812249857951No Hit
AAGCGGGGGGAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAAAAAC8380.11585162696882098No Hit
AAGCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8180.11308667167123576No Hit
AAGCGGGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGC8170.1129484239063565No Hit
AAGCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGT8090.11184244178732242No Hit
AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG7760.10728026554630678No Hit
AAGCGGGGGATCTGCCGCCGTAACCTAGATATTGAGCGTCCAACCTATACCA7660.10589778789751415No Hit
AAGCGGGGGATCGCTTTCTTCCTTCTCTGAAGTGTTAACACTTATGGAAGGA7410.10244159377553264No Hit
AAGCGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC7380.10202685048089485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC351.0188705E-746.00000433
GATTGAG351.0188705E-746.0000049
CTTAACC206.309895E-446.029
TAATATC206.309895E-446.016
AACCGTT600.046.043
CTCACGA206.309895E-446.015
AATTTAC206.309895E-446.013
CTCGTAC206.309895E-446.018
AATCACG253.4158904E-546.030
CGAGTCA301.8604405E-646.037
CTATCGT206.309895E-446.033
CCGATAT253.4158904E-546.030
TAGACGG301.8604405E-646.037
TAGACAT206.309895E-446.046
ATAATTA301.8604405E-646.010
TGGAATC600.046.031
TTATGCG206.309895E-446.028
TAGCGAT206.309895E-446.022
CCGGATT253.4158904E-546.016
ACCGATT253.4158904E-546.040