##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527046_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 723339 Sequences flagged as poor quality 0 Sequence length 52 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22356875545215 33.0 31.0 34.0 31.0 34.0 2 32.86102228692218 34.0 31.0 34.0 31.0 34.0 3 32.82154702013855 34.0 31.0 34.0 31.0 34.0 4 36.21448587729958 37.0 37.0 37.0 35.0 37.0 5 36.26427995725379 37.0 37.0 37.0 35.0 37.0 6 36.31120263113146 37.0 37.0 37.0 35.0 37.0 7 36.61555093808021 37.0 37.0 37.0 35.0 37.0 8 36.63815997754856 37.0 37.0 37.0 35.0 37.0 9 38.4989970124658 39.0 39.0 39.0 37.0 39.0 10 35.620443526479285 35.0 35.0 39.0 33.0 39.0 11 34.83602294359906 35.0 35.0 39.0 30.0 39.0 12 34.64129958428897 35.0 33.0 39.0 27.0 39.0 13 34.858185442786855 35.0 33.0 39.0 30.0 39.0 14 35.544423845527476 35.0 35.0 40.0 31.0 40.0 15 35.7806173868684 35.0 35.0 40.0 32.0 40.0 16 35.91606148707591 35.0 35.0 40.0 32.0 40.0 17 35.87101345289 35.0 35.0 40.0 32.0 40.0 18 35.84200907181833 35.0 35.0 40.0 32.0 40.0 19 35.718173636427736 35.0 35.0 40.0 32.0 40.0 20 35.58704286648446 35.0 35.0 40.0 31.0 40.0 21 31.268283612524694 35.0 32.0 39.0 9.0 40.0 22 31.608695231419848 35.0 32.0 39.0 12.0 40.0 23 33.401526808315325 35.0 32.0 38.0 23.0 40.0 24 34.3250135828429 35.0 33.0 38.0 28.0 40.0 25 34.70412351608305 35.0 34.0 38.0 30.0 40.0 26 34.734030655059385 35.0 34.0 38.0 31.0 40.0 27 34.63930605151941 35.0 34.0 37.0 30.0 40.0 28 34.42051652129914 35.0 34.0 37.0 30.0 40.0 29 34.34868713010082 35.0 34.0 37.0 30.0 39.0 30 34.23293227656742 35.0 34.0 37.0 30.0 39.0 31 33.9931290860855 35.0 34.0 36.0 30.0 39.0 32 33.9679375783692 35.0 33.0 36.0 30.0 39.0 33 33.56121127161676 35.0 33.0 35.0 29.0 39.0 34 33.48166765513819 35.0 33.0 35.0 28.0 38.0 35 33.34659958885115 35.0 33.0 35.0 28.0 37.0 36 33.27660059805983 35.0 33.0 35.0 28.0 37.0 37 33.149353207832014 35.0 33.0 35.0 27.0 37.0 38 33.08355003670478 35.0 33.0 35.0 27.0 37.0 39 32.938971906671696 35.0 33.0 35.0 27.0 37.0 40 28.43843896153809 34.0 26.0 35.0 8.0 36.0 41 29.065124374601673 34.0 25.0 35.0 10.0 36.0 42 30.699167333712133 34.0 27.0 35.0 21.0 35.0 43 31.68799553183224 34.0 30.0 35.0 23.0 35.0 44 32.50670985526841 35.0 33.0 35.0 26.0 35.0 45 32.91941261289658 35.0 33.0 35.0 27.0 35.0 46 33.03036612155573 35.0 33.0 35.0 29.0 35.0 47 33.0504936136445 35.0 33.0 35.0 29.0 35.0 48 32.952171803262374 35.0 33.0 35.0 29.0 35.0 49 32.94053963632543 35.0 33.0 35.0 27.0 35.0 50 32.85674075364386 35.0 33.0 35.0 27.0 35.0 51 32.77560452291387 35.0 33.0 35.0 27.0 35.0 52 32.40961706751606 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 2.0 14 12.0 15 19.0 16 80.0 17 235.0 18 422.0 19 764.0 20 1245.0 21 1852.0 22 2681.0 23 3795.0 24 5054.0 25 6852.0 26 8660.0 27 11183.0 28 14465.0 29 19309.0 30 27051.0 31 42867.0 32 59002.0 33 70688.0 34 157849.0 35 111367.0 36 88925.0 37 66935.0 38 18602.0 39 3422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.72701458099175 1.1388850870753546 0.06456170619861504 0.06953862573426843 8 98.74249833065824 0.9761674678124641 0.18649623482212352 0.09483796670717326 9 93.20235740088673 4.602682836125247 1.3635377050041542 0.8314220579838775 10 56.156380341720826 29.858752258622857 5.402860899246412 8.582006500409904 11 34.35595205014523 22.15558679955042 21.504854570263735 21.983606580040618 12 26.413617957831665 16.289042896898962 29.277282159540686 28.02005698572868 13 25.197175874659045 15.224396859563774 30.35063780606327 29.22778945971391 14 24.291514766935006 16.893185629421335 32.32661310948256 26.488686494161108 15 25.84597263523742 17.85442233862684 28.58051342454921 27.71909160158653 16 27.968352321663843 17.01028148627407 24.91943611501661 30.10193007704548 17 28.61714908224221 19.146209453658656 22.958391570204288 29.27824989389484 18 28.85106430041792 17.62976972069804 24.116907839892498 29.402258138991538 19 29.84409799554566 17.50285827253888 21.54149022795674 31.11155350395872 20 29.796540764427192 18.40686040708437 24.709437760165013 27.08716106832343 21 36.913120957116924 16.06756997756239 23.137007682428294 23.882301382892393 22 32.58430694321749 16.64005397192741 22.550837159340226 28.22480192551487 23 28.201299805485398 19.473580160892748 24.915288682070234 27.409831351551624 24 29.135992943834083 19.89426810942034 23.463825398602868 27.505913548142715 25 27.119234549775417 19.631320860619986 23.99096412608749 29.258480463517106 26 29.06106265526952 20.007078285561818 23.980872039251306 26.950987019917356 27 31.671595199484614 17.22746872489939 22.642633675220054 28.45830240039594 28 30.037644866376624 19.83620404817105 21.761718917409404 28.364432168042924 29 30.029902991543384 18.375754659986534 21.815497297947438 29.778845050522644 30 28.713507774363055 18.66275701987588 22.760420770897184 29.863314434863874 31 31.694820823984326 19.496252794332946 22.227060893993 26.581865487689726 32 33.43259522851664 18.822847931606066 21.51342593168625 26.23113090819104 33 31.072982377557413 20.06279213480816 22.41010093469314 26.454124552941288 34 29.812715752918063 21.44264307606807 24.607825652978754 24.136815518035114 35 32.22956317853731 21.913238467717072 21.69453050367808 24.162667850067535 36 33.102874309279606 21.253520133713238 19.354963578626343 26.288641978380817 37 30.53768703194491 21.83139579090855 20.685460067824355 26.945457109322184 38 31.808183991185324 20.77573585829051 22.104296878780215 25.311783271743955 39 30.62796282241107 20.926425922008907 21.60715791627439 26.838453339305634 40 29.8138217350371 26.55905460648465 20.67827118405063 22.94885247442762 41 28.147383177182483 20.690713482889766 22.496367539977797 28.665535799949954 42 28.71502849977673 20.01246994839211 24.816165034651803 26.45633651717936 43 30.907776298526695 18.322114527213383 24.643631824082483 26.126477350177442 44 26.889466764546082 18.515523150279467 25.564638433708126 29.030371651466325 45 27.816141532531773 18.695521740152266 26.038413523949348 27.44992320336661 46 30.501189622016785 20.090441687784015 22.672356944669097 26.736011745530103 47 28.126369516920835 20.153206173039198 25.283857223238343 26.436567086801627 48 28.64618111286686 20.672050034631066 24.379716840927973 26.3020520115741 49 27.306975014481456 21.19725329340738 25.1801713995789 26.31560029253227 50 27.70471383403909 21.830704552084153 22.661158875713877 27.80342273816288 51 27.23052400050322 23.162168775636317 21.995357640055353 27.611949583805107 52 27.95231558093785 21.65540638621725 23.97644811077517 26.415829922069733 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 4.5 12 9.0 13 31.0 14 145.5 15 238.0 16 557.0 17 876.0 18 638.5 19 401.0 20 603.0 21 805.0 22 989.0 23 1173.0 24 1018.0 25 863.0 26 1168.0 27 1473.0 28 1784.5 29 2096.0 30 2690.0 31 3284.0 32 2836.0 33 2388.0 34 3020.0 35 3652.0 36 4056.5 37 4461.0 38 9204.5 39 11926.0 40 9904.0 41 10390.0 42 10876.0 43 11479.0 44 12082.0 45 12972.5 46 13863.0 47 15338.5 48 16814.0 49 18901.0 50 20988.0 51 22562.0 52 24136.0 53 27672.0 54 31208.0 55 33549.0 56 35890.0 57 39325.0 58 42760.0 59 50874.5 60 58989.0 61 67229.5 62 75470.0 63 70761.0 64 64391.0 65 62730.0 66 58166.5 67 53603.0 68 50314.5 69 47026.0 70 39396.5 71 31767.0 72 27717.0 73 23667.0 74 20721.0 75 17775.0 76 18657.5 77 19540.0 78 14232.5 79 8925.0 80 5675.5 81 2426.0 82 1664.0 83 902.0 84 532.0 85 162.0 86 100.5 87 39.0 88 23.5 89 7.5 90 7.0 91 6.0 92 5.0 93 2.5 94 0.0 95 2.5 96 5.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 723339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.871379425676848 #Duplication Level Percentage of deduplicated Percentage of total 1 65.78318873656039 9.125035707957407 2 12.62450334923788 3.5023855203601357 3 6.106585664443814 2.5412030024069727 4 3.3126825077172066 1.8380590392539218 5 1.9362119272549134 1.3428965145736957 6 1.2454924130228 1.0366018699704647 7 0.8816406711166437 0.8560700586317138 8 0.6207470021177581 0.6888493754981482 9 0.41383133470724975 0.516637031576268 >10 3.4875785671154262 11.815125630007117 >50 1.929213710098185 19.414653924821277 >100 1.5823550793188739 37.65432604460941 >500 0.06197474094615931 5.555303990872413 >1k 0.01299470374677534 3.2965768721575346 >5k 9.995925959057953E-4 0.8162754173035293 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCGGGGGAGTGTGCATCTTAATTTTATTTTCTCCATTTCAGTTGCTTGAT 5887 0.8138645918442113 No Hit AAGCGGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 3388 0.46838342741093736 No Hit AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG 2904 0.40147150920937485 No Hit AAGCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 2824 0.3904116880190339 No Hit AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG 2163 0.2990299154338422 No Hit AAGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 1990 0.27511305210973 No Hit AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 1739 0.24041286312503543 No Hit AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 1677 0.23184150170252124 No Hit AAGCGGGGGGACGCAGAGCCCTTGTACCCTTTATCTGGCTTTCCCCAGTGCT 1351 0.18677273035188202 No Hit AAGCGGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG 1299 0.17958384657816043 No Hit AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 1267 0.17515991810202408 No Hit AAGCGGGGGACCCGGTTCCTTCTTTGCTTACAAAGGCTTGCTCCTTCCTCCA 1063 0.14695737406665477 No Hit AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA 1056 0.14598963971249995 No Hit AAGCGGGGGGTCTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG 1054 0.14571314418274142 No Hit AAGCGGGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT 958 0.13244135875433236 No Hit AAGCGGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT 917 0.12677320039428264 No Hit AAGCGGGGGATCGTGTTGAGTGTAAGCTGTTGTAGTGATGTTAGCTATGTAA 902 0.1246994839210937 No Hit AAGCGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 899 0.12428474062645592 No Hit AAGCGGGGAGAGGAGCCCGAGGTCCGGGCCTTTGACCCAGACACAGCAGCCG 896 0.12386999733181815 No Hit AAGCGGGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC 895 0.12373174956693887 No Hit AAGCGGGGGGAGGGTCTGTGGCTAGGTGCAGACTGGGTGCAGAACATCACAA 879 0.1215197853288707 No Hit AAGCGGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG 840 0.11612812249857951 No Hit AAGCGGGGGGAGTTATTAAACAAACAAACAGAAAAAAATTGTTTTGAAAAAC 838 0.11585162696882098 No Hit AAGCGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT 818 0.11308667167123576 No Hit AAGCGGGGGGAGCTCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGC 817 0.1129484239063565 No Hit AAGCGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGT 809 0.11184244178732242 No Hit AAGCGGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG 776 0.10728026554630678 No Hit AAGCGGGGGATCTGCCGCCGTAACCTAGATATTGAGCGTCCAACCTATACCA 766 0.10589778789751415 No Hit AAGCGGGGGATCGCTTTCTTCCTTCTCTGAAGTGTTAACACTTATGGAAGGA 741 0.10244159377553264 No Hit AAGCGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC 738 0.10202685048089485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.764955297585226E-4 0.0 0.0 0.0 0.0 8 4.147432946377839E-4 0.0 0.0 0.0 0.0 9 9.677343541548292E-4 0.0 0.0 0.0 0.0 10 0.004700424005894884 0.0 0.0 0.0 0.0 11 0.011198068955220167 0.0 0.0 0.0 0.0 12 0.013824776487926132 0.0 0.0 0.0 0.0 13 0.017557466139666186 0.0 0.0 0.0 0.0 14 0.024055111088991468 0.0 0.0 0.0 0.0 15 0.027787800740731523 0.0 0.0 0.0 0.0 16 0.03552967557397016 0.0 0.0 0.0 0.0 17 0.03898586969595169 0.0 0.0 0.0 0.0 18 0.04299505487745027 0.0 0.0 0.0 0.0 19 0.04534526688039771 0.0 0.0 0.0 0.0 20 0.04935445206189629 0.0 0.0 0.0 0.0 21 0.05391662830291191 0.0 0.0 0.0 0.0 22 0.057372822424893444 0.0 0.0 0.0 0.0 23 0.05986128219272015 0.0 0.0 0.0 0.0 24 0.06387046737421873 0.0 0.0 0.0 0.0 25 0.06705016596644174 0.0 0.0 0.0 0.0 26 0.07119759891281957 0.0 0.0 0.0 0.0 27 0.07520678409431815 0.0 0.0 0.0 0.0 28 0.08046019915973009 0.0 0.0 0.0 0.0 29 0.08557536646026276 0.0 0.0 0.0 0.0 30 0.08903156058224429 0.0 0.0 0.0 0.0 31 0.09428497564765621 0.0 0.0 0.0 0.0 32 0.0996766384779474 0.0 0.0 0.0 0.0 33 0.10396231918920451 0.0 0.0 0.0 0.0 34 0.11156594625756389 0.0 0.0 0.0 0.0 35 0.11834008673664768 0.0 0.0 0.0 0.0 36 0.1215197853288707 0.0 0.0 0.0 0.0 37 0.1276026869835582 0.0 0.0 0.0 0.0 38 0.13175011992993604 0.0 0.0 0.0 0.0 39 0.13603580064119314 0.0 0.0 0.0 0.0 40 0.1454366486529829 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACC 35 1.0188705E-7 46.000004 33 GATTGAG 35 1.0188705E-7 46.000004 9 CTTAACC 20 6.309895E-4 46.0 29 TAATATC 20 6.309895E-4 46.0 16 AACCGTT 60 0.0 46.0 43 CTCACGA 20 6.309895E-4 46.0 15 AATTTAC 20 6.309895E-4 46.0 13 CTCGTAC 20 6.309895E-4 46.0 18 AATCACG 25 3.4158904E-5 46.0 30 CGAGTCA 30 1.8604405E-6 46.0 37 CTATCGT 20 6.309895E-4 46.0 33 CCGATAT 25 3.4158904E-5 46.0 30 TAGACGG 30 1.8604405E-6 46.0 37 TAGACAT 20 6.309895E-4 46.0 46 ATAATTA 30 1.8604405E-6 46.0 10 TGGAATC 60 0.0 46.0 31 TTATGCG 20 6.309895E-4 46.0 28 TAGCGAT 20 6.309895E-4 46.0 22 CCGGATT 25 3.4158904E-5 46.0 16 ACCGATT 25 3.4158904E-5 46.0 40 >>END_MODULE