FastQCFastQC Report
Fri 17 Jun 2016
SRR1527045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611423
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC58010.3599923794062763No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34840.21620642128106649No Hit
TTTAGGGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTT25720.15961048092276206No Hit
TTTAGGGGGCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCT21150.13125045379146258No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT20670.12827172008839394No Hit
TTTAGGGGATGGGCCAGGCCACGGAACACAGGAGACCTTCGAGTCAGCCC20050.12442418905526359No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG19570.12144545535219492No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT19380.12026637326139691No Hit
TTTAGGGGCAGTGAGCAGACTTTTCATTCCTCTGGTTCTGTGGTTGTCTC19330.11995608850066058No Hit
TTTAGGGGGGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCT18750.11635678527611931No Hit
TTTAGGGGGGTGTTTGTGTATTTATGCTTTGATTCATAGTAACTTCTCAT18400.11418479195096508No Hit
TTTAGGGGGATCATTTCCATTGTATTTATTACAGAATGTTTTAGCCTTGA18280.11344010852519792No Hit
TTTAGGGGGGTCGCCTGCCTCTGAAGTGGCATGGTCAGACGCGCCAGCAT17980.11157839996078002No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17590.10915817882703673No Hit
TTTAGGGGAGTGGCATGATGGTGACCTGTGCAGTCACTGAGCTGTAGGAG16820.10437979351169743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG207.857425E-444.00000414
GTCGCAT551.8189894E-1244.00000436
GTCGCAA207.857425E-444.00000440
CGAACGT207.857425E-444.00000438
CGAACCG207.857425E-444.00000431
GCGACTA207.857425E-444.00000421
GAATGCG207.857425E-444.00000414
TAGACGC207.857425E-444.00000424
TAGCGTA207.857425E-444.00000418
GACCGTA207.857425E-444.00000440
TAGCGGC551.8189894E-1244.00000439
GTCTCCG207.857425E-444.00000419
GCACGTA207.857425E-444.00000427
GCACGAC207.857425E-444.00000430
CCGCTAG207.857425E-444.00000419
TACGCAC207.857425E-444.0000049
TGCGCGA207.857425E-444.00000412
GGACGTA408.3164196E-944.0000048
AATTCGC207.857425E-444.00000411
GCGATAG207.857425E-444.00000415
Produced by FastQC (version 0.11.5)