##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1611423
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33659194389059	33.0	31.0	34.0	31.0	34.0
2	32.83734376386585	34.0	31.0	34.0	31.0	34.0
3	33.25306825085654	34.0	34.0	34.0	31.0	34.0
4	36.17614369411383	37.0	35.0	37.0	35.0	37.0
5	36.29545687259025	37.0	37.0	37.0	35.0	37.0
6	36.36324850768544	37.0	37.0	37.0	35.0	37.0
7	36.66720159759418	37.0	37.0	37.0	35.0	37.0
8	36.700938859629034	37.0	37.0	37.0	35.0	37.0
9	38.56667243796321	39.0	39.0	39.0	38.0	39.0
10	36.589245033737264	37.0	35.0	39.0	34.0	39.0
11	36.00339327414341	39.0	35.0	39.0	31.0	39.0
12	36.10088040198011	39.0	35.0	39.0	32.0	39.0
13	36.260700635401136	38.0	35.0	39.0	33.0	39.0
14	37.01120562384923	39.0	35.0	40.0	33.0	40.0
15	37.09439420934168	39.0	35.0	40.0	33.0	40.0
16	37.02514175359294	39.0	35.0	40.0	33.0	40.0
17	37.10896456113634	39.0	35.0	40.0	33.0	40.0
18	37.13486837410165	39.0	35.0	40.0	33.0	40.0
19	37.115003323149786	39.0	35.0	40.0	33.0	40.0
20	36.881805087801276	39.0	35.0	40.0	33.0	40.0
21	37.021316563062584	39.0	35.0	40.0	33.0	40.0
22	36.91402257507805	39.0	35.0	40.0	33.0	40.0
23	37.020511684393234	39.0	35.0	40.0	33.0	40.0
24	37.05591579616277	39.0	35.0	40.0	33.0	40.0
25	37.018903168193575	39.0	35.0	40.0	33.0	40.0
26	36.95906661379414	39.0	35.0	40.0	33.0	40.0
27	36.85573310049565	38.0	35.0	40.0	33.0	40.0
28	36.746910029210206	38.0	35.0	40.0	33.0	40.0
29	36.84055583170899	39.0	35.0	40.0	33.0	40.0
30	36.83748463314723	39.0	35.0	40.0	33.0	40.0
31	36.81501939590039	39.0	35.0	40.0	33.0	40.0
32	36.06006120056621	38.0	35.0	40.0	32.0	40.0
33	36.320046319309085	38.0	35.0	40.0	32.0	40.0
34	36.36845074198395	38.0	35.0	40.0	33.0	40.0
35	36.41770472433371	38.0	35.0	40.0	33.0	40.0
36	36.28196320891535	38.0	35.0	40.0	33.0	40.0
37	36.27659962654126	38.0	35.0	40.0	33.0	40.0
38	36.12406053531568	37.0	35.0	40.0	32.0	40.0
39	36.1213300294212	37.0	35.0	40.0	32.0	40.0
40	36.140586922242015	37.0	35.0	40.0	33.0	40.0
41	36.02547996398214	37.0	35.0	40.0	32.0	40.0
42	35.971446355178	37.0	35.0	40.0	32.0	40.0
43	35.94589068171424	37.0	35.0	40.0	32.0	40.0
44	35.80806467327325	36.0	35.0	40.0	32.0	40.0
45	35.75108211810307	36.0	35.0	40.0	32.0	40.0
46	35.52115242242416	36.0	35.0	40.0	31.0	40.0
47	35.47370677965996	36.0	35.0	40.0	31.0	40.0
48	35.45014251379061	36.0	35.0	40.0	31.0	40.0
49	35.232498232928286	35.0	35.0	40.0	30.0	40.0
50	34.887864328608934	35.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	17.0
15	39.0
16	106.0
17	225.0
18	436.0
19	752.0
20	1133.0
21	1832.0
22	2628.0
23	3746.0
24	5151.0
25	7197.0
26	9544.0
27	12760.0
28	16297.0
29	20464.0
30	25343.0
31	33275.0
32	45499.0
33	75693.0
34	210672.0
35	192194.0
36	122343.0
37	159738.0
38	382417.0
39	281920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.26003290259602	0.5876793368345866	0.1354703265374765	0.016817434031908444
8	98.8185597450204	0.9160226706457584	0.17742082618902671	0.08799675814481982
9	91.69789682783478	4.125856463510822	2.80398132582196	1.3722653828324407
10	33.2841842272327	53.42526450224428	5.9770153460636966	7.313535924459313
11	27.541806217237806	23.05341303928267	35.164199592534054	14.24058115094547
12	28.082508441296916	18.187589478367876	29.859633379938106	23.8702687003971
13	23.916004674129635	20.349405463369955	31.87654638167632	23.858043480824094
14	23.02989345441886	23.73951470222282	29.953711719393354	23.27688012396497
15	22.068445094801305	27.648171833218214	29.850573064924603	20.432810007055878
16	26.186978837958748	30.06994439076518	26.410632093497487	17.332444677778586
17	25.692633157153644	29.198540668713306	25.672278476849343	19.436547697283704
18	23.153014447479027	29.020002817385627	26.63850522178224	21.188477513353103
19	22.172514603552266	29.84356062933196	27.44865873206476	20.53526603505101
20	22.82212677862982	30.286212868998398	26.883257841051044	20.00840251132074
21	21.56187419442319	29.537557798293808	31.145639599285847	17.754928407997156
22	21.553372391979014	28.35500051817555	30.557401749881937	19.5342253399635
23	19.379331187403928	32.46025407357348	30.630939238176445	17.529475500846146
24	21.125799991684367	32.859962902354006	27.556389600992414	18.45784750496921
25	20.522854644621553	35.66400628512811	25.68028382367634	18.13285524657399
26	20.81433614885725	36.03336926430863	24.783064409531203	18.369230177302917
27	19.552656254751234	32.707488970928175	27.067877273689156	20.67197750063143
28	20.219644376430026	33.60979705514939	25.606311936716804	20.56424663170378
29	21.16135862526475	33.02230388917125	25.590301243062807	20.226036242501195
30	20.582615489539368	31.829569268900844	27.59033475381697	19.997480487742823
31	21.12642056120584	33.33246453600327	25.812589245654305	19.72852565713658
32	22.050200350870007	33.341711021873216	25.96090536128627	18.64718326597051
33	21.877930251709206	33.540045040935865	26.234204178542814	18.34782052881211
34	20.631268140022822	32.76582250594661	28.0546448697828	18.548264484247774
35	19.871939273548907	35.594316327866736	26.15613653274156	18.377607865842798
36	20.31316420331595	35.05348998990333	25.781002257011348	18.852343549769365
37	20.858520698786105	35.26113255178808	24.95527245173986	18.92507429768596
38	22.051317376008658	35.05051125620027	24.147787390399667	18.75038397739141
39	20.295664142810423	34.819659394212444	25.039794020564432	19.8448824424127
40	20.30546914124969	36.08040843403625	24.354809382762937	19.25931304195112
41	18.79078305323928	36.77135053924388	25.238438324387825	19.19942808312901
42	19.876779715816394	35.19529012555983	25.26189585229949	19.66603430632429
43	19.27178648933272	35.5667009841612	25.83859110860401	19.322921417902066
44	20.432375608390846	35.2686414429979	25.084412969158315	19.214569979452943
45	20.255947693436173	35.63297780905448	23.969125425167693	20.14194907234165
46	19.358542108434595	37.156972439886985	24.295420879558005	19.18906457212042
47	18.78302593422087	36.06805910055895	26.560437575980984	18.5884773892392
48	19.474898893710716	36.730703235587434	25.060893384294502	18.733504486407355
49	19.88813613805934	35.81449439408523	24.509020908848886	19.788348559006543
50	19.070908135232028	36.59666021894934	24.543586631194913	19.78884501462372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	135.0
6	268.0
7	854.0
8	1440.0
9	3946.0
10	6452.0
11	15655.0
12	24858.0
13	31492.0
14	38126.0
15	33127.0
16	28128.0
17	27754.0
18	27380.0
19	26036.5
20	24693.0
21	24183.5
22	23674.0
23	24579.0
24	25484.0
25	27634.5
26	29785.0
27	30159.0
28	30533.0
29	35380.0
30	40227.0
31	50648.5
32	61070.0
33	63635.0
34	66200.0
35	65055.0
36	63910.0
37	68248.5
38	72587.0
39	77232.0
40	81877.0
41	78216.0
42	74555.0
43	79567.0
44	84579.0
45	86772.5
46	88966.0
47	89387.5
48	89809.0
49	90008.5
50	90208.0
51	85237.5
52	80267.0
53	86085.0
54	91903.0
55	88441.5
56	84980.0
57	82045.5
58	79111.0
59	71113.5
60	63116.0
61	57351.5
62	51587.0
63	43222.0
64	34857.0
65	28253.0
66	21649.0
67	17547.0
68	13445.0
69	9855.0
70	6265.0
71	4922.0
72	3579.0
73	3390.5
74	3202.0
75	2118.0
76	1034.0
77	1029.5
78	1025.0
79	675.0
80	325.0
81	231.0
82	137.0
83	116.5
84	96.0
85	63.0
86	30.0
87	17.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1611423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.038419877502085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7136306663072	5.603874344808826
2	9.972918692943232	1.6033301571613399
3	4.119444931982146	0.9934148407656153
4	2.1150534718302514	0.6800675147976035
5	1.2146364949725903	0.4881879072563566
6	0.7249365622471101	0.3496406683137118
7	0.49062919456526777	0.27607184290534004
8	0.36553712012687345	0.23506726819141813
9	0.28216275001574925	0.20413283925755252
>10	4.165874591938243	9.40768841810535
>50	3.138232927503731	18.353052245283685
>100	3.5558623924410093	51.83732551320016
>500	0.10741333647139209	5.6492391908856066
>1k	0.0328652745919931	3.945117866403136
>5k	8.015920632193439E-4	0.3737893826643026
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5801	0.3599923794062763	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3484	0.21620642128106649	No Hit
TTTAGGGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTT	2572	0.15961048092276206	No Hit
TTTAGGGGGCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCT	2115	0.13125045379146258	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2067	0.12827172008839394	No Hit
TTTAGGGGATGGGCCAGGCCACGGAACACAGGAGACCTTCGAGTCAGCCC	2005	0.12442418905526359	No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1957	0.12144545535219492	No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1938	0.12026637326139691	No Hit
TTTAGGGGCAGTGAGCAGACTTTTCATTCCTCTGGTTCTGTGGTTGTCTC	1933	0.11995608850066058	No Hit
TTTAGGGGGGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCT	1875	0.11635678527611931	No Hit
TTTAGGGGGGTGTTTGTGTATTTATGCTTTGATTCATAGTAACTTCTCAT	1840	0.11418479195096508	No Hit
TTTAGGGGGATCATTTCCATTGTATTTATTACAGAATGTTTTAGCCTTGA	1828	0.11344010852519792	No Hit
TTTAGGGGGGTCGCCTGCCTCTGAAGTGGCATGGTCAGACGCGCCAGCAT	1798	0.11157839996078002	No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1759	0.10915817882703673	No Hit
TTTAGGGGAGTGGCATGATGGTGACCTGTGCAGTCACTGAGCTGTAGGAG	1682	0.10437979351169743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0011170251386507454	0.0	0.0	0.0	0.0
8	0.002482278085890545	0.0	0.0	0.0	0.0
9	0.004406043602455717	0.0	0.0	0.0	0.0
10	0.012969902998778099	0.0	0.0	0.0	0.0
11	0.017500060505528343	0.0	0.0	0.0	0.0
12	0.022774901438045753	0.0	0.0	0.0	0.0
13	0.028670311892035796	0.0	0.0	0.0	0.0
14	0.037358285192652704	0.0	0.0	0.0	0.0
15	0.04437072078529349	0.0	0.0	0.0	0.0
16	0.053431035798793984	0.0	0.0	0.0	0.0
17	0.059264389300636766	0.0	0.0	6.205695214726362E-5	0.0
18	0.06497362889818502	0.0	0.0	6.205695214726362E-5	0.0
19	0.07105521020861685	0.0	0.0	6.205695214726362E-5	0.0
20	0.077447076279785	0.0	0.0	6.205695214726362E-5	0.0
21	0.08272191721230242	0.0	0.0	6.205695214726362E-5	0.0
22	0.08855527071414519	0.0	0.0	6.205695214726362E-5	0.0
23	0.09445068116813525	0.0	0.0	3.723417128835818E-4	0.0
24	0.09991169295709444	0.0	0.0	3.723417128835818E-4	0.0
25	0.10711029940617703	0.0	0.0	3.723417128835818E-4	0.0
26	0.11468124756814319	0.0	0.0	3.723417128835818E-4	0.0
27	0.1220039679215203	0.0	0.0	3.723417128835818E-4	0.0
28	0.1322433650258188	0.0	0.0	3.723417128835818E-4	0.0
29	0.14223453432152824	0.0	0.0	3.723417128835818E-4	0.0
30	0.15433563999024466	0.0	0.0	3.723417128835818E-4	0.0
31	0.16575411918534116	0.0	0.0	3.723417128835818E-4	0.0
32	0.1764279149546705	0.0	0.0	3.723417128835818E-4	0.0
33	0.18927370404915408	0.0	0.0	3.723417128835818E-4	0.0
34	0.20143686667001776	0.0	0.0	3.723417128835818E-4	0.0
35	0.21726138946756995	0.0	0.0	3.723417128835818E-4	0.0
36	0.22824546999763562	0.0	0.0	3.723417128835818E-4	0.0
37	0.2397260061448794	0.0	0.0	3.723417128835818E-4	0.0
38	0.2499654032491779	0.0	0.0	3.723417128835818E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	7.857425E-4	44.000004	14
GTCGCAT	55	1.8189894E-12	44.000004	36
GTCGCAA	20	7.857425E-4	44.000004	40
CGAACGT	20	7.857425E-4	44.000004	38
CGAACCG	20	7.857425E-4	44.000004	31
GCGACTA	20	7.857425E-4	44.000004	21
GAATGCG	20	7.857425E-4	44.000004	14
TAGACGC	20	7.857425E-4	44.000004	24
TAGCGTA	20	7.857425E-4	44.000004	18
GACCGTA	20	7.857425E-4	44.000004	40
TAGCGGC	55	1.8189894E-12	44.000004	39
GTCTCCG	20	7.857425E-4	44.000004	19
GCACGTA	20	7.857425E-4	44.000004	27
GCACGAC	20	7.857425E-4	44.000004	30
CCGCTAG	20	7.857425E-4	44.000004	19
TACGCAC	20	7.857425E-4	44.000004	9
TGCGCGA	20	7.857425E-4	44.000004	12
GGACGTA	40	8.3164196E-9	44.000004	8
AATTCGC	20	7.857425E-4	44.000004	11
GCGATAG	20	7.857425E-4	44.000004	15
>>END_MODULE