FastQCFastQC Report
Fri 17 Jun 2016
SRR1527044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712940
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA65010.3795229254965148No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC31900.18622952350928812No Hit
TTTAGGGGGCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTC22490.13129473303209685No Hit
TTTAGGGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTA22030.12860929162726073No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC20790.12137027566639812No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT19470.11366422641773792No Hit
TTTAGGGGATGGGCCAGGCCACGGAACACAGGAGACCTTCGAGTCAGCCCTG19460.11360584725676323No Hit
TTTAGGGGGGTCGCCTGCCTCTGAAGTGGCATGGTCAGACGCGCCAGCATTG18030.10525762723738133No Hit
TTTAGGGGGGTGTTTGTGTATTTATGCTTTGATTCATAGTAACTTCTCATGT17970.10490735227153315No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT17910.10455707730568496No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17200.10041215687648136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG351.0199619E-746.00000441
GTCGCAA351.0199619E-746.00000440
CGTTCGC700.046.00000446
AACGAGC351.0199619E-746.00000417
CGTCGTT351.0199619E-746.00000419
CGCGTCA700.046.00000428
AATCGCG700.046.00000425
AACGTAT600.046.040
CGGGTAC253.4178625E-546.036
TACCCCG253.4178625E-546.030
CGAACGG206.3123263E-446.012
GCGAACC253.4178625E-546.029
TTTCGCA301.8619412E-646.043
CGTGACG453.110472E-1046.019
CGTCTCG206.3123263E-446.042
CCGTCTA301.8619412E-646.031
ATCGTTA206.3123263E-446.010
ATCCGCG501.6370905E-1146.010
ATCCGAC206.3123263E-446.041
CCCGTAG206.3123263E-446.037
Produced by FastQC (version 0.11.5)