##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1712940
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.320359148598314	33.0	31.0	34.0	31.0	34.0
2	32.861616869242354	34.0	31.0	34.0	31.0	34.0
3	33.223310215185585	34.0	34.0	34.0	31.0	34.0
4	36.08195908788399	37.0	35.0	37.0	35.0	37.0
5	36.265980711525216	37.0	37.0	37.0	35.0	37.0
6	36.379173818113884	37.0	37.0	37.0	35.0	37.0
7	36.65659976414819	37.0	37.0	37.0	35.0	37.0
8	36.65351442549068	37.0	37.0	37.0	35.0	37.0
9	38.50910539773722	39.0	39.0	39.0	37.0	39.0
10	36.4810851518442	37.0	35.0	39.0	33.0	39.0
11	35.88559260686306	39.0	35.0	39.0	31.0	39.0
12	36.0095292304459	38.0	35.0	39.0	32.0	39.0
13	36.212955503403506	38.0	35.0	39.0	33.0	39.0
14	36.94411771574019	39.0	35.0	40.0	33.0	40.0
15	36.957678611043	39.0	35.0	40.0	33.0	40.0
16	32.023532639788904	37.0	33.0	40.0	3.0	40.0
17	33.52488703632351	37.0	33.0	40.0	17.0	40.0
18	35.54969934732098	37.0	34.0	40.0	29.0	40.0
19	36.55045185470595	38.0	35.0	40.0	31.0	40.0
20	36.8489818674326	39.0	35.0	40.0	33.0	40.0
21	37.03400878022581	39.0	35.0	40.0	33.0	40.0
22	37.12462783284879	39.0	35.0	40.0	33.0	40.0
23	37.119602554672085	39.0	35.0	40.0	33.0	40.0
24	37.089565308767384	39.0	35.0	40.0	33.0	40.0
25	37.03456571742151	39.0	35.0	40.0	33.0	40.0
26	36.97574988032272	39.0	35.0	40.0	33.0	40.0
27	36.8671056779572	38.0	35.0	40.0	33.0	40.0
28	36.79485387696008	38.0	35.0	40.0	33.0	40.0
29	36.57335166438988	38.0	35.0	40.0	33.0	40.0
30	36.535876913377	38.0	35.0	40.0	33.0	40.0
31	36.50474856095368	38.0	35.0	40.0	33.0	40.0
32	36.47016299461744	38.0	35.0	40.0	33.0	40.0
33	36.17475276425327	38.0	35.0	40.0	32.0	40.0
34	36.13058951276752	37.0	35.0	40.0	32.0	40.0
35	36.13659380947377	37.0	35.0	40.0	32.0	40.0
36	36.13509171366189	37.0	35.0	40.0	32.0	40.0
37	35.920796408514015	37.0	35.0	40.0	31.0	40.0
38	35.92755029364718	37.0	35.0	40.0	31.0	40.0
39	35.57805585718122	36.0	35.0	40.0	31.0	40.0
40	35.68015575560148	36.0	35.0	40.0	31.0	40.0
41	35.639623687928356	36.0	35.0	40.0	31.0	40.0
42	35.65600021016498	36.0	35.0	40.0	31.0	40.0
43	35.62221035179282	36.0	35.0	40.0	31.0	40.0
44	35.74305463121884	36.0	35.0	40.0	31.0	40.0
45	35.81361343654769	36.0	35.0	40.0	32.0	40.0
46	35.79081929314512	36.0	35.0	40.0	32.0	40.0
47	35.492553738017676	36.0	35.0	40.0	31.0	40.0
48	35.518702931801464	36.0	35.0	40.0	31.0	40.0
49	35.48768316461756	35.0	35.0	40.0	31.0	40.0
50	35.005367380060015	35.0	35.0	40.0	29.0	40.0
51	35.07249932863965	35.0	35.0	40.0	30.0	40.0
52	34.83334792812358	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	4.0
14	10.0
15	31.0
16	97.0
17	213.0
18	406.0
19	713.0
20	1201.0
21	1918.0
22	2727.0
23	4174.0
24	5978.0
25	8295.0
26	11253.0
27	14668.0
28	18736.0
29	24267.0
30	32325.0
31	44119.0
32	66110.0
33	119526.0
34	211242.0
35	190438.0
36	158811.0
37	230380.0
38	343829.0
39	221467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.25461487267505	0.5828575431713895	0.1455392483099233	0.016988335843637254
8	98.80141744602847	0.915210106600348	0.1913668896750616	0.09200555769612478
9	91.7021028173783	4.082162831155791	2.7934428526393216	1.422291498826579
10	33.3357268789333	53.30846381075811	5.9893516410382155	7.366457669270378
11	27.543346527023715	23.035249337396525	35.12259623804687	14.298807897532898
12	28.030287108713676	18.18516702277955	29.91926162037199	23.865284248134785
13	23.89762630331477	20.32884981377048	31.896271906780154	23.877251976134602
14	23.091760365220033	23.733288965170992	29.80740714794447	23.36754352166451
15	22.135042675166673	27.728992258923256	29.66601282006375	20.469952245846322
16	25.200123763821264	36.460179574299154	23.21628311557906	15.123413546300512
17	25.670192767989537	29.146204770745033	25.721449671325324	19.462152789940106
18	23.260826415402757	28.913972468387684	26.5237544806006	21.301446635608954
19	22.125526871927796	29.897719709972325	27.442175441054562	20.534577977045316
20	22.775637208542037	30.255408829264308	26.933809707286887	20.03514425490677
21	21.68091118194449	29.398402746155732	31.056254159515216	17.864431912384553
22	21.224911555571126	28.33566849977232	30.7265870374911	19.71283290716546
23	19.384041472555957	32.42238490548414	30.612514156946535	17.581059465013368
24	21.248088082478077	32.58070919004752	27.626711968895584	18.544490758578817
25	20.715845271871753	35.4644646047147	25.601305358039394	18.21838476537415
26	20.763832942192955	35.8564806706598	24.84803904398286	18.531647343164384
27	19.59730054759653	32.6324331266711	27.055413499597186	20.714852826135182
28	20.30368839539038	33.3929384566885	25.633005242448654	20.67036790547246
29	21.48329772204514	32.84785223066774	25.373685009399043	20.295165037888076
30	20.705337022896305	31.715705161885417	27.583277873130406	19.99567994208787
31	21.180601772391327	33.12503648697561	25.92133991850269	19.77302182213037
32	21.915069996614008	33.25971721134424	26.168167011103716	18.657045780938038
33	22.38151949280185	33.132100365453546	26.220299601854123	18.26608053989048
34	20.675330134155313	32.55572290915035	28.156561233901943	18.612385722792393
35	20.047520637033404	35.42517542937873	26.131855172977453	18.39544876061041
36	20.303104603780632	34.88797039008955	25.83493876026014	18.973986245869675
37	21.11031326257779	34.97769916050766	24.90606792999171	19.005919646922834
38	21.922367391735843	34.99783997104394	24.262029026118835	18.81776361110138
39	20.810944925099538	34.47604703025208	24.964038436839587	19.748969607808796
40	20.40007239015961	35.77089682067089	24.429518838955246	19.39951195021425
41	18.738776606302615	36.636192744637874	25.15038471867082	19.474645930388686
42	19.90606792999171	34.877520520275084	25.301703503917246	19.914708045815964
43	19.44598176235011	35.3384823753313	25.814622812240945	19.400913050077644
44	20.399138323584012	35.05738671523813	25.159375109460925	19.384099851716933
45	20.280103214356604	35.513503099933445	23.962952584445457	20.24344110126449
46	19.073989748619333	37.24105923149673	24.448492066272024	19.23645895361192
47	19.099851716931123	35.68945789111119	26.65738438007169	18.553306011885997
48	19.608801242308544	36.58832183263862	25.105899798008103	18.696977127044732
49	19.8640933132509	35.76990437493433	24.480075192359337	19.88592711945544
50	19.1871285625883	36.28504209137506	24.456606769647507	20.071222576389133
51	18.545191308510514	36.626676941399	25.137366165773468	19.69076558431702
52	19.034642194122387	37.893096080423135	24.679498406248907	18.392763319205578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	145.0
6	285.0
7	870.0
8	1455.0
9	3900.5
10	6346.0
11	15947.5
12	25549.0
13	32346.5
14	34436.0
15	29728.0
16	28895.5
17	28063.0
18	26499.0
19	24935.0
20	24249.5
21	23564.0
22	24741.0
23	25918.0
24	27346.0
25	28774.0
26	28835.5
27	28897.0
28	34342.5
29	39788.0
30	43906.0
31	48024.0
32	54081.5
33	60139.0
34	62406.0
35	64673.0
36	66670.5
37	68668.0
38	72622.0
39	78528.5
40	80481.0
41	80823.5
42	81166.0
43	83135.5
44	85105.0
45	87889.0
46	90673.0
47	91504.0
48	92335.0
49	90834.5
50	89334.0
51	89565.0
52	89796.0
53	92775.0
54	95754.0
55	94195.5
56	92637.0
57	83527.5
58	74418.0
59	72518.0
60	70618.0
61	62765.5
62	54913.0
63	44540.5
64	29977.5
65	25787.0
66	20340.0
67	14893.0
68	11740.5
69	8588.0
70	7044.0
71	5500.0
72	4362.0
73	3224.0
74	2331.5
75	1439.0
76	938.5
77	438.0
78	596.0
79	754.0
80	528.5
81	303.0
82	177.0
83	51.0
84	37.0
85	23.0
86	14.5
87	6.0
88	5.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1712940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.08880362714995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49410976676882	7.515564448162837
2	9.075199258156315	1.8311580638559195
3	3.4206835668698874	1.0353181433030745
4	1.67451931895382	0.6757558631517585
5	0.9383217908065175	0.47332721432613145
6	0.5681468883726634	0.343915343290085
7	0.36851777952567005	0.26025324575234776
8	0.27133131964949664	0.2189926721471379
9	0.19030502901146398	0.1727955060260161
>10	3.5830493106307	10.191213356797455
>50	2.5779674821678817	18.905721323319334
>100	2.7355895465146802	49.376467726720676
>500	0.07685703964017435	4.957340506181733
>1k	0.024750572087513772	3.614986191415486
>5k	6.513308444082572E-4	0.4271903955499986
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6501	0.3795229254965148	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3190	0.18622952350928812	No Hit
TTTAGGGGGCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTC	2249	0.13129473303209685	No Hit
TTTAGGGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTA	2203	0.12860929162726073	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2079	0.12137027566639812	No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1947	0.11366422641773792	No Hit
TTTAGGGGATGGGCCAGGCCACGGAACACAGGAGACCTTCGAGTCAGCCCTG	1946	0.11360584725676323	No Hit
TTTAGGGGGGTCGCCTGCCTCTGAAGTGGCATGGTCAGACGCGCCAGCATTG	1803	0.10525762723738133	No Hit
TTTAGGGGGGTGTTTGTGTATTTATGCTTTGATTCATAGTAACTTCTCATGT	1797	0.10490735227153315	No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1791	0.10455707730568496	No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1720	0.10041215687648136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.254124487722862E-4	0.0	0.0	0.0	0.0
8	0.0015178581853421602	0.0	0.0	0.0	0.0
9	0.003094095531659019	0.0	0.0	0.0	0.0
10	0.012434761287610775	0.0	0.0	0.0	0.0
11	0.016579681716814367	0.0	0.0	0.0	0.0
12	0.019440260604574593	0.0	0.0	0.0	0.0
13	0.02527817670204444	0.0	0.0	0.0	0.0
14	0.037946454633554005	0.0	0.0	0.0	0.0
15	0.0458276413651383	0.0	0.0	0.0	0.0
16	0.05452613635036837	0.0	0.0	0.0	0.0
17	0.061356498184408095	0.0	0.0	0.0	0.0
18	0.06748631008675143	0.0	0.0	0.0	0.0
19	0.07431667192079115	0.0	0.0	0.0	0.0
20	0.0802713463402104	0.0	0.0	0.0	0.0
21	0.08733522481814891	0.0	0.0	0.0	0.0
22	0.09270610762782118	0.0	0.0	0.0	0.0
23	0.09860240288626572	0.0	0.0	0.0	0.0
24	0.10368138989106448	0.0	0.0	0.0	0.0
25	0.11127068081777529	0.0	0.0	0.0	0.0
26	0.11705021775427044	0.0	0.0	0.0	0.0
27	0.12341354630051257	0.0	0.0	0.0	0.0
28	0.13333800366621132	0.0	0.0	0.0	0.0
29	0.1421532569733908	0.0	0.0	0.0	0.0
30	0.15131878524641842	0.0	0.0	0.0	0.0
31	0.16229406750966174	0.0	0.0	0.0	0.0
32	0.1718098707485376	0.0	0.0	0.0	0.0
33	0.18190946559716042	0.0	0.0	0.0	0.0
34	0.19189230212383387	0.0	0.0	0.0	0.0
35	0.20368489264072298	0.0	0.0	0.0	0.0
36	0.21244176678692775	0.0	0.0	0.0	0.0
37	0.22318353240627226	0.0	0.0	0.0	0.0
38	0.2338669188646421	0.0	0.0	0.0	0.0
39	0.24145620979135288	0.0	0.0	0.0	0.0
40	0.25032984225950705	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	35	1.0199619E-7	46.000004	41
GTCGCAA	35	1.0199619E-7	46.000004	40
CGTTCGC	70	0.0	46.000004	46
AACGAGC	35	1.0199619E-7	46.000004	17
CGTCGTT	35	1.0199619E-7	46.000004	19
CGCGTCA	70	0.0	46.000004	28
AATCGCG	70	0.0	46.000004	25
AACGTAT	60	0.0	46.0	40
CGGGTAC	25	3.4178625E-5	46.0	36
TACCCCG	25	3.4178625E-5	46.0	30
CGAACGG	20	6.3123263E-4	46.0	12
GCGAACC	25	3.4178625E-5	46.0	29
TTTCGCA	30	1.8619412E-6	46.0	43
CGTGACG	45	3.110472E-10	46.0	19
CGTCTCG	20	6.3123263E-4	46.0	42
CCGTCTA	30	1.8619412E-6	46.0	31
ATCGTTA	20	6.3123263E-4	46.0	10
ATCCGCG	50	1.6370905E-11	46.0	10
ATCCGAC	20	6.3123263E-4	46.0	41
CCCGTAG	20	6.3123263E-4	46.0	37
>>END_MODULE