FastQCFastQC Report
Fri 17 Jun 2016
SRR1527043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663982
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA62690.3767468638482868No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC30290.18203321910934134No Hit
TTTAGGGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTA21800.13101103257126578No Hit
TTTAGGGGGCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTC21190.12734512753142763No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC19780.11887147817704759No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT19410.11664789643157199No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT18310.11003724799907692No Hit
TTTAGGGGGGTCGCCTGCCTCTGAAGTGGCATGGTCAGACGCGCCAGCATTG17200.10336650276265007No Hit
TTTAGGGGGGTGTTTGTGTATTTATGCTTTGATTCATAGTAACTTCTCATGT17170.10318621235085475No Hit
TTTAGGGGATGGGCCAGGCCACGGAACACAGGAGACCTTCGAGTCAGCCCTG17030.10234485709580993No Hit
TTTAGGGGGGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCTTG16670.10018137215426609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT301.8619085E-646.038
TCCGCTA405.6134013E-946.028
CGAGTAC253.4178207E-546.018
CAACGGG405.6134013E-946.029
CGACGGT253.4178207E-546.041
CCGATAT206.3122745E-446.038
AACCCGC206.3122745E-446.025
ACGCAAT405.6134013E-946.016
ACCGCTA206.3122745E-446.019
TTTCGTC206.3122745E-446.045
CGGCACG206.3122745E-446.046
GAATCGT206.3122745E-446.011
CGTCTAA206.3122745E-446.021
CGTTCGC551.8189894E-1246.046
TCTAGCG253.4178207E-546.045
TCGGCGA253.4178207E-546.023
AGTATCG551.8189894E-1246.040
TACGCAC206.3122745E-446.09
GTTAACG206.3122745E-446.015
TATTCGC301.8619085E-646.017
Produced by FastQC (version 0.11.5)