##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1663982
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27480405437078	33.0	31.0	34.0	31.0	34.0
2	32.821145901818646	34.0	31.0	34.0	31.0	34.0
3	33.18780551712699	34.0	34.0	34.0	31.0	34.0
4	36.08950817977598	37.0	35.0	37.0	35.0	37.0
5	36.287548182612554	37.0	37.0	37.0	35.0	37.0
6	36.36893127449696	37.0	37.0	37.0	35.0	37.0
7	36.66300777292062	37.0	37.0	37.0	35.0	37.0
8	36.680452072197895	37.0	37.0	37.0	35.0	37.0
9	38.449530103090055	39.0	39.0	39.0	37.0	39.0
10	36.438319044316586	37.0	35.0	39.0	33.0	39.0
11	35.75758932488452	39.0	35.0	39.0	31.0	39.0
12	35.93960271204857	38.0	35.0	39.0	31.0	39.0
13	36.100041947569146	38.0	35.0	39.0	32.0	39.0
14	36.8601553382188	39.0	35.0	40.0	33.0	40.0
15	37.04702695101269	39.0	35.0	40.0	33.0	40.0
16	37.15782021680523	39.0	35.0	40.0	33.0	40.0
17	37.154610446507235	39.0	35.0	40.0	33.0	40.0
18	37.17522124638367	39.0	35.0	40.0	33.0	40.0
19	37.14898598662726	39.0	35.0	40.0	33.0	40.0
20	37.106085883140565	39.0	35.0	40.0	33.0	40.0
21	32.836090174052366	37.0	33.0	40.0	10.0	40.0
22	33.529624719498166	37.0	34.0	40.0	16.0	40.0
23	35.61433056367196	37.0	34.0	40.0	29.0	40.0
24	36.45893585387342	38.0	35.0	40.0	32.0	40.0
25	36.75700278007815	38.0	35.0	40.0	33.0	40.0
26	36.8670838987441	38.0	35.0	40.0	33.0	40.0
27	36.834626215908585	38.0	35.0	40.0	33.0	40.0
28	36.773936256522006	38.0	35.0	40.0	33.0	40.0
29	36.72647901239316	38.0	35.0	40.0	33.0	40.0
30	36.631127019402854	38.0	35.0	40.0	33.0	40.0
31	36.501240398033154	38.0	35.0	40.0	33.0	40.0
32	36.48600285339625	38.0	35.0	40.0	33.0	40.0
33	36.21002030070037	38.0	35.0	40.0	32.0	40.0
34	36.146823102653755	37.0	35.0	40.0	32.0	40.0
35	36.14876422942075	37.0	35.0	40.0	32.0	40.0
36	36.08082960032019	37.0	35.0	40.0	32.0	40.0
37	36.06602595460768	37.0	35.0	40.0	32.0	40.0
38	36.00962570508575	37.0	35.0	40.0	32.0	40.0
39	35.89773507165342	36.0	35.0	40.0	32.0	40.0
40	31.159717472905356	35.0	32.0	40.0	8.0	40.0
41	32.010043377873075	35.0	31.0	40.0	13.0	40.0
42	33.934222846160594	35.0	32.0	40.0	23.0	40.0
43	34.90881511939432	35.0	34.0	40.0	29.0	40.0
44	35.47250871704141	36.0	34.0	40.0	31.0	40.0
45	35.70710199990144	36.0	35.0	40.0	31.0	40.0
46	35.76551008364273	36.0	35.0	40.0	32.0	40.0
47	35.711209616450176	36.0	35.0	40.0	32.0	40.0
48	35.63523343401551	36.0	35.0	40.0	31.0	40.0
49	35.54252089265389	35.0	35.0	40.0	31.0	40.0
50	35.43972170372035	35.0	35.0	40.0	31.0	40.0
51	35.25952263906701	35.0	35.0	40.0	31.0	40.0
52	34.95406260404259	35.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	1.0
14	10.0
15	28.0
16	108.0
17	232.0
18	459.0
19	787.0
20	1216.0
21	2003.0
22	2954.0
23	4459.0
24	6256.0
25	8742.0
26	11714.0
27	15094.0
28	19689.0
29	26288.0
30	35808.0
31	55276.0
32	85565.0
33	81340.0
34	221680.0
35	204548.0
36	187748.0
37	139931.0
38	328097.0
39	223945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.2528765335202	0.588227516884197	0.144472716651983	0.014423232943625592
8	98.83099696991916	0.9017525429962583	0.17325908573530244	0.09399140134929344
9	91.68212156141112	4.069455078240029	2.8440812460711715	1.4043421142776786
10	33.41304172761484	53.21415736468303	6.025185368591727	7.347615539110398
11	27.649517843342053	23.010405160632747	35.09238681668432	14.247690179340882
12	28.152407898643133	18.13391010239293	29.867510586052013	23.846171412911918
13	24.05795254996749	20.29463059095591	31.872159674804173	23.775257184272427
14	23.124048216867727	23.735352906461728	29.845575252616914	23.295023624053627
15	22.02031031585678	27.613940535414443	29.856392677324635	20.50935647140414
16	26.06001747615058	30.103871315915676	26.419276170054722	17.416835037879018
17	25.69222503608813	29.141661388164053	25.714340659935026	19.45177291581279
18	23.3025357245451	29.025734653379665	26.433819596606213	21.237910025469027
19	22.154927156663952	29.99581726244635	27.345848693074803	20.503406887814894
20	22.810703481167465	30.33842914166139	26.91687770660981	19.93398967056134
21	27.365620541568358	27.334430300327767	28.850492373114612	16.449456784989263
22	21.284004274084694	28.38065555997601	30.631581351240577	19.703758814698716
23	19.3666758414454	32.4734882949455	30.610246985844796	17.549588877764304
24	21.390676101063594	32.60035264804547	27.52301407106567	18.485957179825263
25	20.72943096740229	35.558918305606674	25.525636695589256	18.186014031401783
26	20.82149927102577	35.87454671985634	24.82809309235316	18.475860916764724
27	19.566257327302818	32.693682984551515	27.02192691988255	20.718132768263118
28	20.252562828203672	33.55685337942357	25.559711583418572	20.630872208954184
29	21.31134831987365	33.021090372371816	25.387954917781563	20.27960638997297
30	20.687483398257914	31.73027112072126	27.585394553546855	19.996850927473975
31	21.218919435426585	33.13785846241125	25.88705887443494	19.75616322772722
32	21.925838140076035	33.31682674452007	26.156292556049284	18.601042559354607
33	22.27770492709657	33.258653038314115	26.228468817571343	18.235173217017973
34	20.588203478162626	32.66675961639008	28.14375395887696	18.601282946570336
35	20.03807733497117	35.45537151243223	26.101724658079235	18.40482649451737
36	20.386578700971526	34.96690469007477	25.78910108402615	18.857415524927553
37	20.973544184973157	35.11432215011941	24.974849487554554	18.937284177352883
38	21.957569252552013	35.06323986677741	24.1512227896696	18.827968091000987
39	20.429968593410265	34.746950387684485	24.95844306008118	19.864637958824076
40	22.121813817697547	38.104258339333	22.268149535271416	17.505778307698037
41	18.80753517766418	36.77491703636217	25.04967000844961	19.367877777524036
42	19.890900262142257	35.03541504655699	25.192700401807233	19.880984289493515
43	19.39894782515676	35.4691336805326	25.732009120290968	19.39990937401967
44	20.39324944620795	35.15542836400874	25.154899512134143	19.296422677649158
45	20.322455411176325	35.616851624596904	23.87946504229012	20.181227921936657
46	18.974664389398445	37.36747152312946	24.409158272144772	19.24870581532733
47	18.811681857135472	35.96348998967537	26.60371326132134	18.621114891867823
48	19.512290397372087	36.665841337226	25.070884180237528	18.750984085164383
49	19.900395557163478	35.87400584862096	24.342270529368708	19.883328064846857
50	19.00272959683458	36.64138193802577	24.450324582838036	19.90556388230161
51	18.63193231657554	36.74522921521988	25.04822768515525	19.574610783049337
52	19.030674610662857	37.937669998834124	24.625867347122746	18.405788043380277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	4.0
4	8.0
5	123.0
6	238.0
7	801.5
8	1365.0
9	3599.0
10	5833.0
11	14543.5
12	23254.0
13	29668.5
14	32591.0
15	29099.0
16	28568.0
17	28037.0
18	26437.5
19	24838.0
20	24302.0
21	23766.0
22	24417.0
23	25068.0
24	26695.0
25	28322.0
26	27932.0
27	27542.0
28	32610.5
29	37679.0
30	41761.0
31	45843.0
32	51755.5
33	57668.0
34	59845.5
35	62023.0
36	64748.0
37	67473.0
38	71228.0
39	76743.0
40	78503.0
41	78768.5
42	79034.0
43	80837.5
44	82641.0
45	85269.5
46	87898.0
47	88313.0
48	88728.0
49	87831.0
50	86934.0
51	87250.5
52	87567.0
53	89828.0
54	92089.0
55	91130.5
56	90172.0
57	81550.0
58	72928.0
59	71173.0
60	69418.0
61	62277.5
62	55137.0
63	44684.0
64	29512.0
65	24793.0
66	19672.0
67	14551.0
68	11476.0
69	8401.0
70	7004.5
71	5608.0
72	4345.0
73	3082.0
74	2332.0
75	1582.0
76	1008.5
77	435.0
78	586.0
79	737.0
80	525.5
81	314.0
82	185.5
83	57.0
84	36.5
85	16.0
86	8.5
87	1.0
88	0.5
89	0.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1663982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.791363891137175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8415680082872	7.328010265524304
2	8.29831444788968	1.625036328847379
3	3.176153693481918	0.9329662976118248
4	1.564209115954189	0.612629626245658
5	0.9482125523799073	0.46421470732478204
6	0.5556166613560886	0.32641469491897185
7	0.3892240421827606	0.2667723962533514
8	0.28606413434398453	0.2240766428452083
9	0.23064563824738535	0.2032501836585363
>10	3.9964032615871745	11.047182100038093
>50	2.814219047448196	20.019787687659672
>100	2.802156081284147	48.60025878175464
>500	0.07521614196407181	4.851452271829156
>1k	0.021287587348322208	3.0623893592079523
>5k	7.095862449440736E-4	0.4355586562804785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTAGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6269	0.3767468638482868	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3029	0.18203321910934134	No Hit
TTTAGGGGGAGCATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTA	2180	0.13101103257126578	No Hit
TTTAGGGGGCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTC	2119	0.12734512753142763	No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1978	0.11887147817704759	No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1941	0.11664789643157199	No Hit
TTTAGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1831	0.11003724799907692	No Hit
TTTAGGGGGGTCGCCTGCCTCTGAAGTGGCATGGTCAGACGCGCCAGCATTG	1720	0.10336650276265007	No Hit
TTTAGGGGGGTGTTTGTGTATTTATGCTTTGATTCATAGTAACTTCTCATGT	1717	0.10318621235085475	No Hit
TTTAGGGGATGGGCCAGGCCACGGAACACAGGAGACCTTCGAGTCAGCCCTG	1703	0.10234485709580993	No Hit
TTTAGGGGGGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCTTG	1667	0.10018137215426609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.206776275224131E-4	0.0	0.0	0.0	0.0
8	0.002343775353339159	0.0	0.0	0.0	0.0
9	0.004447163490951225	0.0	0.0	0.0	0.0
10	0.014122748923966725	0.0	0.0	0.0	0.0
11	0.019050686846372136	0.0	0.0	0.0	0.0
12	0.023197366317664492	0.0	0.0	0.0	0.0
13	0.029086853102978278	0.0	0.0	0.0	0.0
14	0.03948360018317506	0.0	0.0	0.0	0.0
15	0.04699570067464672	0.0	0.0	0.0	0.0
16	0.05498857559757257	0.0	0.0	0.0	0.0
17	0.06069777197109103	0.0	0.0	0.0	0.0
18	0.06544541948170113	0.0	0.0	0.0	0.0
19	0.07121471265915136	0.0	0.0	0.0	0.0
20	0.07800565150344174	0.0	0.0	0.0	0.0
21	0.08389513828875553	0.0	0.0	0.0	0.0
22	0.08948414105441044	0.0	0.0	0.0	0.0
23	0.09561401505545132	0.0	0.0	0.0	0.0
24	0.10018137215426609	0.0	0.0	0.0	0.0
25	0.10637134295923874	0.0	0.0	0.0	0.0
26	0.113462765823188	0.0	0.0	0.0	0.0
27	0.12061428549106902	0.0	0.0	0.0	0.0
28	0.1320927750420377	0.0	0.0	0.0	0.0
29	0.13990535955316824	0.0	0.0	0.0	0.0
30	0.14976123539797906	0.0	0.0	0.0	0.0
31	0.15949691763492635	0.0	0.0	0.0	0.0
32	0.1703744391465773	0.0	0.0	0.0	0.0
33	0.18041060540318346	0.0	0.0	0.0	0.0
34	0.18984580362047185	0.0	0.0	0.0	0.0
35	0.20084351873998638	0.0	0.0	0.0	0.0
36	0.210218620153343	0.0	0.0	0.0	0.0
37	0.22007449599815382	0.0	0.0	0.0	0.0
38	0.22975008143116932	0.0	0.0	0.0	0.0
39	0.2366011170793915	0.0	0.0	0.0	0.0
40	0.2453151536494986	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	30	1.8619085E-6	46.0	38
TCCGCTA	40	5.6134013E-9	46.0	28
CGAGTAC	25	3.4178207E-5	46.0	18
CAACGGG	40	5.6134013E-9	46.0	29
CGACGGT	25	3.4178207E-5	46.0	41
CCGATAT	20	6.3122745E-4	46.0	38
AACCCGC	20	6.3122745E-4	46.0	25
ACGCAAT	40	5.6134013E-9	46.0	16
ACCGCTA	20	6.3122745E-4	46.0	19
TTTCGTC	20	6.3122745E-4	46.0	45
CGGCACG	20	6.3122745E-4	46.0	46
GAATCGT	20	6.3122745E-4	46.0	11
CGTCTAA	20	6.3122745E-4	46.0	21
CGTTCGC	55	1.8189894E-12	46.0	46
TCTAGCG	25	3.4178207E-5	46.0	45
TCGGCGA	25	3.4178207E-5	46.0	23
AGTATCG	55	1.8189894E-12	46.0	40
TACGCAC	20	6.3122745E-4	46.0	9
GTTAACG	20	6.3122745E-4	46.0	15
TATTCGC	30	1.8619085E-6	46.0	17
>>END_MODULE