FastQCFastQC Report
Fri 17 Jun 2016
SRR1527042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306005
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC14650.47875034721654874No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC11830.38659499027793665No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC10960.35816408228623714No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC10440.3411708959003938No Hit
CCTTTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA4900.16012810248198558No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC4390.14346170814202383No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC4300.1405205797290894No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT3520.11503080015032434No Hit
CCTTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3230.10555383081975786No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC3220.10522703877387625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGC202.002226E-458.03392450
TTAAGAC150.004685249657.99590342
TAAGTCG258.674862E-657.97691337
TTATAAC150.004691329357.97691336
CGTAGCG258.733969E-657.9105512
TCGTATC150.004712654357.91054515
ACGTAGC303.7927384E-757.91054511
AGTCGTA150.004712654357.91054513
CGCGAAT303.7970312E-757.90107727
TCGCGAA303.7970312E-757.90107726
GACGTAG303.814239E-757.86323510
TTTCGGG301600.057.6473
CTTTCGG304250.057.641812
TTCGGGG203450.057.519494
TCGGGGG117750.057.407535
CCTTTCG307000.057.37241
TTCGGGA69550.057.0613944
TCGGGAT19800.056.6798825
TTCGGGT11300.056.6045154
TCGGGAC12150.056.473225
Produced by FastQC (version 0.11.5)