FastQCFastQC Report
Fri 17 Jun 2016
SRR1527041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396993
Sequences flagged as poor quality0
Sequence length64
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC34930.8798643804802603No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC28360.7143702785691436No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC27050.681372215631006No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC23530.5927056648354001No Hit
CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT18300.4609653066930651No Hit
CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC17540.4418213923167411No Hit
CCTTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT12620.3178897360910646No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC11850.29849392810452574No Hit
CCTTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC9110.2294750789056734No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT8730.2199031217175114No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC8230.20730844120677192No Hit
CCTTTCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG6610.166501676351976No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC6420.16171569775789496No Hit
CCTTTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA6020.15163995334930339No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC5430.1367782303466308No Hit
CCTTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC5430.1367782303466308No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTCC5320.1340074006342681No Hit
CCTTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT5280.13299982619340894No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT5270.13274793258319417No Hit
CCTTTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5240.1319922517525498No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA5210.1312365709219054No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCT5160.12997710287083147No Hit
CCTTTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG5040.126954379548254No Hit
CCTTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA4720.11889378402138073No Hit
CCTTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA4610.11612295430901805No Hit
CCTTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4580.11536727347837368No Hit
CCTTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4450.11209265654558141No Hit
CCTTTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC4410.11108508210472225No Hit
CCTTTCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG4110.10352827379827856No Hit
CCTTTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA4040.10176501852677503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAT150.004619125358.20768755
ATAAACC150.004619125358.20768755
AATCGAT150.00463305558.16343752
CATGCGA201.9822424E-458.1560749
CGCATAT201.9822424E-458.1560749
GTCAGTA201.9822424E-458.1560749
TATTCGG150.004635379658.15606751
GTGTCGT150.004635379658.15606750
ACTAACG150.004635379658.15606749
CGATATA150.00465400858.0971941
TCGGTTA150.004682054758.00909830
AACGGTT150.004686741657.99444229
TCGTTTA202.0210267E-457.9285824
CATAACG150.004722003457.88475821
TTTCGGG395050.057.2256163
CTTTCGG397950.057.150332
CCTTTCG399100.057.1309471
TTCGGGG267750.057.0164264
TCGGGGG150950.056.8925635
TTCGGGA88950.056.471394
Produced by FastQC (version 0.11.5)