FastQCFastQC Report
Fri 17 Jun 2016
SRR1527040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401070
Sequences flagged as poor quality0
Sequence length64
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC33260.829281671528661No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC26970.6724511930585683No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC26030.6490138878500012No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC21770.5427980153090483No Hit
CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT16730.41713416610566734No Hit
CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC15830.394694193033635No Hit
CCTTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT11030.2750143366494627No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC10570.2635450170793128No Hit
CCTTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC9540.23786371456354255No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT8000.19946642730695388No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC7970.19871842820455282No Hit
CCTTTCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG6340.15807714364076098No Hit
CCTTTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA6330.15782781060662726No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC6120.15259181688981974No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTCC5700.14211982945620463No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA5290.13189717505672327No Hit
CCTTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC5090.1269105143740494No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCT5060.12616251527164835No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT5030.12541451616924726No Hit
CCTTTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4950.12341985189617774No Hit
CCTTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT4950.12341985189617774No Hit
CCTTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4740.11818385817937019No Hit
CCTTTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG4650.11593986087216696No Hit
CCTTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA4650.11593986087216696No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC4470.11145186625776049No Hit
CCTTTCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG4440.1107038671553594No Hit
CCTTTCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC4300.10721320467748771No Hit
CCTTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC4070.1014785448924128No Hit
CCTTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA4040.10073054579001171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGT150.00454064758.4602240
ATCGCGA150.00456795758.37175426
CTATCGG150.00467378158.0351150
TCGTAAC150.00469004357.98421538
AATGCGC150.004725032557.8754734
ACTAGAC150.004725032557.8754733
GCGGAAA150.00472737257.86823331
GCCAATT150.004779060457.70951512
TTTCGGG398450.057.259083
CTTTCGG402250.057.120572
TTCGGGG267850.057.0334244
CCTTTCG404300.057.010031
TTCGGGA90000.056.9753764
TCGGGGG149100.056.7829325
TCGGGGA53400.056.781955
TTCGGGC27900.056.7743874
TCGGGAT26200.056.3761865
TTCGGGT14850.055.669294
TCGGGGC41500.055.511935
TCGGGGT26650.055.3157845
Produced by FastQC (version 0.11.5)