FastQCFastQC Report
Fri 17 Jun 2016
SRR1527039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368661
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC73371.9901752558583632No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC40301.0931451930092957No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC25490.691421115876103No Hit
CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC19580.5311112376953353No Hit
CCTTTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT14630.3968415427723573No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC9380.25443429058132No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT8570.2324628859575599No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT8160.22134155769121225No Hit
CCTTTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC7780.2110339851516705No Hit
CCTTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7170.19448761870661666No Hit
CCTTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC7120.1931313591619401No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC7100.1925888553440695No Hit
CCTTTCGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT6430.17441497744540377No Hit
CCTTTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC5680.1540710842752556No Hit
CCTTTCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC5480.1486460460965494No Hit
CCTTTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5220.14159349646423136No Hit
CCTTTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCA5110.13860972546594297No Hit
CCTTTCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4930.13372719110510742No Hit
CCTTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4620.12531838192811282No Hit
CCTTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG4560.12369087047450096No Hit
CCTTTCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT4530.12287711474769504No Hit
CCTTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC4370.11853708420473008No Hit
CCTTTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC4170.1131120460260239No Hit
CCTTTCGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGC3970.10768700784731773No Hit
CCTTTCGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC3870.10497448875796463No Hit
CCTTTCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT3850.10443198494009401No Hit
CCTTTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3760.10199071775967625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA150.00823450.2930843
ATTATAC150.00823846350.28618642
CGCACAA150.00825633350.25863341
CAATCCA150.00828319150.21735840
CATATCA301.0237363E-650.1555736
CGAATGT204.1864417E-449.9847429
TCGCGAA204.2090067E-449.93032526
ATACGGT150.00850497849.882824
CCTTATG150.00855077849.8150722
AGATACG150.00855077849.8150722
ACGGGTT204.2658424E-449.7947920
CGGGTTA402.7775968E-949.7880336
CGGGTAT1150.049.788036
TTATCCG301.0800431E-649.7677715
ATCCGGT301.0800431E-649.7677717
CGAATGA204.2772823E-449.7677714
ACGTTTT150.00858754949.7610211
GCGATAG150.00858754949.7610210
GGCGATA150.00858754949.761029
CGATAGC204.280146E-449.7610211
Produced by FastQC (version 0.11.5)