FastQCFastQC Report
Fri 17 Jun 2016
SRR1527038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42620
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGCAGTCACTCTCCTGGTCACAAGAGGAAGGAGACACCTAGTCCCCGTTCTAATCGT2070.4856874706710464No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.3871421867667762No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.2979821679962459No Hit
CAACAAGGGAAGAAACACTGCTATCAAGATTTTTAAAGCTCAAACCAAGTGACACTTTTAATAA850.1994368840919756No Hit
CAACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC840.19709056780854056No Hit
CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.19474425152510558No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCCGTGGTTAAGAGCACTGACTGCTCTTCCGGAGGTCCT790.18535898639136555No Hit
CAACAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.16658845612388548No Hit
CAACAAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTC700.1642421398404505No Hit
CAACAAGGGATGTCATTCTGTGCATCTGGGGTTATCTGGGTCTTCAGTACTCTGGGAAACAGAT630.14781792585640546No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC610.14312529328953544No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC560.1313937118723604No Hit
CAACAAGGGCAAGCTAGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGTA560.1313937118723604No Hit
CAACAAGGGATTTCTGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCTAATATCCT550.1290473955889254No Hit
CAACAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.12670107930549038No Hit
CAACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.12200844673862037No Hit
CAACAAGGGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCTTGTTACCAGTCTT490.11496949788831536No Hit
CAACAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.11496949788831536No Hit
CAACAAGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT490.11496949788831536No Hit
CAACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG480.11262318160488033No Hit
CAACAAGGGAACTTGTGTCAGTTCTCTCCTAAGGTAGCTGTCAGAGTTGTTAGTAAAAAGGAGC470.11027686532144533No Hit
CAACAAGGGCGATGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGGGCTCAAGG450.1055842327545753No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC430.10089160018770531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATC150.004409582458.801256
CTAATCG150.004409582458.801257
TAATCGT150.004409582458.801258
ACCATAA201.9474386E-458.24318355
ACAGGTT150.004579541758.2431843
CCCTCAA150.004579541758.2431853
TAAGGCT303.570167E-758.2431843
CGTTCTA150.004579541758.2431853
CCAGAAA150.00460112458.1741741
AAGCTGC150.00460112458.1741740
ACTAAGG351.5537807E-858.17416841
AACTAAG351.5537807E-858.17416840
GAGCGCT201.9704382E-458.10532836
GCTAGGC150.00462278358.10532839
CTGAACT303.6293386E-758.10532837
AGACACC150.00462278358.10532839
GAGACAC150.00462278358.10532838
AGGAGAC150.00462278358.10532836
GCTGAAC351.5685146E-858.10532436
CCATGCT150.004644517358.03664433
Produced by FastQC (version 0.11.5)