FastQCFastQC Report
Fri 17 Jun 2016
SRR1527037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55055
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4990.9063663609118154No Hit
CAACAAGGGCAGTCACTCTCCTGGTCACAAGAGGAAGGAGACACCTAGTCCCCGTTCTAATCGT3280.5957678684951413No Hit
CAACAAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTC3020.5485423667241849No Hit
CAACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2870.5212968849332485No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.48496957587866685No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCCGTGGTTAAGAGCACTGACTGCTCTTCCGGAGGTCCT2460.4468259013713559No Hit
CAACAAGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC1840.3342112433021524No Hit
CAACAAGGGCAGTGCTCAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG1800.32694578149123604No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1750.3178639542275906No Hit
CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1700.30878212696394514No Hit
CAACAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCCAGAGGTCC1650.2997002997002997No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1640.29788393424757065No Hit
CAACAAGGGGATCATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCTAT1590.28880210698392517No Hit
CAACAAGGGAAGAAACACTGCTATCAAGATTTTTAAAGCTCAAACCAAGTGACACTTTTAATAA1550.2815366451730088No Hit
CAACAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1520.2760875488148215No Hit
CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1500.2724548179093634No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1450.26337299064571795No Hit
CAACAAGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT1440.26155662519298883No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.2470257015711561No Hit
CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1310.23794387430751068No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1290.2343111434020525No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1280.2324947779493234No Hit
CAACAAGGGGAAGCAGAGGCATGGGCGGAAGTGAGGGTGAAGCTGCCTCCTTGTACCATAAGTA1270.23067841249659432No Hit
CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.22522931613840705No Hit
CAACAAGGGGGGAGTTTCAGGACAGTGGGACAGTGTCCTCCTGTCTGGGGTGATGTGCACTAGG1150.20888202706384526No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1130.20524929615838705No Hit
CAACAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.1998001998001998No Hit
CAACAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1060.19253473798928344No Hit
CAACAAGGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCCAGAGGTC1010.183452910725638No Hit
CAACAAGGGGGACAAATAAGATTCCTGAGGAATGACTCTCTTAATTGCGCCGCGGTTCCAGCTT980.17800381436745072No Hit
CAACAAGGGCGATGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGGGCTCAAGG970.17618744891472166No Hit
CAACAAGGGATGTCATTCTGTGCATCTGGGGTTATCTGGGTCTTCAGTACTCTGGGAAACAGAT970.17618744891472166No Hit
CAACAAGGGCGAGCTCATGGGAAGGCTGTGCATAGGGTACTCTGAGCGCTGCCTTTTAGTGAGT910.1652892561983471No Hit
CAACAAGGGGCACTGAGGAAGGTTTATTTCTTGGTTGGGGTATCCTGAATGGGGCTTGGGAAAG910.1652892561983471No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG850.15439106348197257No Hit
CAACAAGGGGGATTCCAGAATCAAACGTACTTTATCGTATCAGGCTGTAAGCGCCTCCAAAATT800.14530923621832711No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC790.14349287076559802No Hit
CAACAAGGGGCCAAAGTGCATTGCACAGAGGCCACATCTCGTGTACCTGTGGGTCGTGTGGGAG780.14167650531286896No Hit
CAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC780.14167650531286896No Hit
CAACAAGGGAGGGACTCAGACACAGAGAAGATGGGTAGTCTGCTGGCTGCAGGGTTAACCCTAC760.13804377440741075No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC740.1344110435019526No Hit
CAACAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.12896194714376533No Hit
CAACAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG710.12896194714376533No Hit
CAACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.12714558169103624No Hit
CAACAAGGGAACTTGTGTCAGTTCTCTCCTAAGGTAGCTGTCAGAGTTGTTAGTAAAAAGGAGC700.12714558169103624No Hit
CAACAAGGGATTTCTGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCTAATATCCT680.12351285078557805No Hit
CAACAAGGGCAAGCTAGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGTA680.12351285078557805No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC670.12169648533284898No Hit
CAACAAGGGGCTTCTTTCCGGTTTCCGCGGCAGCCGCAGCCATGAGCAGCAAAGTCTCACGCGA650.11806375442739078No Hit
CAACAAGGGGATTTAGCACCCGTCTAAGTTAATATAAGTAGATAAAGACTGGGACACAGGCTTT640.1162473889746617No Hit
CAACAAGGGACCTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCCGAAG630.11443102352193261No Hit
CAACAAGGGATGGCCTCTCTGGTTTCTCCTTAAGGACAAATATTTGGGATGGAGAAGGAAGACG620.11261465806920352No Hit
CAACAAGGGCAAAATGGCTAACATAGATTTTAGAATCAAACAGTATGTGTCTTTGTTCATCTTC620.11261465806920352No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC620.11261465806920352No Hit
CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA610.11079829261647442No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTCTTCCAAAGGTC610.11079829261647442No Hit
CAACAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGCTAGTAG590.10716556171101625No Hit
CAACAAGGGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCTTGTTACCAGTCTT570.10353283080555807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTGG201.9579277E-458.2111649
AACGCCA201.9579277E-458.2111647
CCAATGT201.9579277E-458.2111651
TGTTCTA201.9579277E-458.2111657
CGCCAAT201.9579277E-458.2111649
GTTTTAG201.9579277E-458.2111648
ATGTGTT201.9579277E-458.2111654
TTGTTAG150.00459683958.21115547
TAGTGTC150.00459683958.21115544
TATACTT150.00459683958.21115556
TAGTGAG150.00459683958.21115557
ATTAGTT150.00459683958.21115556
TGCGAAA150.00459683958.21115544
TAGTTTT150.00459683958.21115558
TCGGAGA150.00459683958.21115551
TCCAGTG150.00459683958.21115546
TTTTAGG150.00459683958.21115549
CATTATG150.00459683958.21115545
ATTATGG150.00459683958.21115546
AGTGTCT150.00459683958.21115545
Produced by FastQC (version 0.11.5)