FastQCFastQC Report
Fri 17 Jun 2016
SRR1527036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54990
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC5110.9292598654300781No Hit
CAACAAGGGCAGTCACTCTCCTGGTCACAAGAGGAAGGAGACACCTAGTCCCCGTTCTAATCGT3240.5891980360065466No Hit
CAACAAGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTC2850.5182760501909438No Hit
CAACAAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2480.45099108928896164No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.4455355519185306No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCCGTGGTTAAGAGCACTGACTGCTCTTCCGGAGGTCCT2290.4164393526095654No Hit
CAACAAGGGCAGTGCTCAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTG1690.3073286052009456No Hit
CAACAAGGGGCTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC1690.3073286052009456No Hit
CAACAAGGGAAGAAACACTGCTATCAAGATTTTTAAAGCTCAAACCAAGTGACACTTTTAATAA1600.29096199308965265No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1590.28914348063284234No Hit
CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1550.281869430805601No Hit
CAACAAGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCCAGAGGTCC1540.2800509183487907No Hit
CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1520.27641389343517003No Hit
CAACAAGGGGATCATCTCAGAGGATCACAAGCTGAAGATTCCAGAAAACGCCAATGTGTTCTAT1490.270958356064739No Hit
CAACAAGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT1490.270958356064739No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1470.2673213311511184No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1390.2527732314966358No Hit
CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1360.24731769412620475No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.2364066193853428No Hit
CAACAAGGGGAAGCAGAGGCATGGGCGGAAGTGAGGGTGAAGCTGCCTCCTTGTACCATAAGTA1290.23458810692853246No Hit
CAACAAGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1280.23276959447172213No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1230.2236770321876705No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1200.2182214948172395No Hit
CAACAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1190.21640298236042915No Hit
CAACAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1180.21458446990361885No Hit
CAACAAGGGGGGAGTTTCAGGACAGTGGGACAGTGTCCTCCTGTCTGGGGTGATGTGCACTAGG1100.20003637024913623No Hit
CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.1982178577923259No Hit
CAACAAGGGGCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1010.18366975813784323No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG860.1563920712856883No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC850.15457355882887797No Hit
CAACAAGGGGCACTGAGGAAGGTTTATTTCTTGGTTGGGGTATCCTGAATGGGGCTTGGGAAAG840.15275504637206766No Hit
CAACAAGGGGGGGGCTGGAGAGTTGGCTCAGCAGTTAGGAACACTGACTGCTCTTCCAGAGGTC830.15093653391525733No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC800.14548099654482632No Hit
CAACAAGGGGCCAAAGTGCATTGCACAGAGGCCACATCTCGTGTACCTGTGGGTCGTGTGGGAG800.14548099654482632No Hit
CAACAAGGGATGTCATTCTGTGCATCTGGGGTTATCTGGGTCTTCAGTACTCTGGGAAACAGAT780.14184397163120568No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC740.13456992180396435No Hit
CAACAAGGGGGACAAATAAGATTCCTGAGGAATGACTCTCTTAATTGCGCCGCGGTTCCAGCTT740.13456992180396435No Hit
CAACAAGGGATTTCTGTTTCCAGATAACTAACTTGTAACCAATCATTTTATTTCCTAATATCCT700.12729587197672304No Hit
CAACAAGGGGATTTAGCACCCGTCTAAGTTAATATAAGTAGATAAAGACTGGGACACAGGCTTT690.1254773595199127No Hit
CAACAAGGGAGGGACTCAGACACAGAGAAGATGGGTAGTCTGCTGGCTGCAGGGTTAACCCTAC690.1254773595199127No Hit
CAACAAGGGCACTGAGGAAGGTTTATTTCTTGGTTGGGGTATCCTGAATGGGGCTTGGGAAAGG690.1254773595199127No Hit
CAACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCTCTTCCAAAGGTC690.1254773595199127No Hit
CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA680.12365884706310237No Hit
CAACAAGGGCAAAATGGCTAACATAGATTTTAGAATCAAACAGTATGTGTCTTTGTTCATCTTC670.12184033460629205No Hit
CAACAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.11638479723586106No Hit
CAACAAGGGTGAGTTTTTTGTAACCAGTCTCTTGTCTCAGGCTCTCTGCCTTGTTACCAGTCTT630.11456628477905073No Hit
CAACAAGGGATGGCCTCTCTGGTTTCTCCTTAAGGACAAATATTTGGGATGGAGAAGGAAGACG600.10911074740861974No Hit
CAACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.10911074740861974No Hit
CAACAAGGGGGGACTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGATCTTCTGGAGGTCC600.10911074740861974No Hit
CAACAAGGGCGAGCTCATGGGAAGGCTGTGCATAGGGTACTCTGAGCGCTGCCTTTTAGTGAGT590.10729223495180942No Hit
CAACAAGGGCGATGTAGGACCGTATAGGTAGATCCAGACCGTGAGATGTTTTAGGGGCTCAAGG590.10729223495180942No Hit
CAACAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.10729223495180942No Hit
CAACAAGGGCAAGCTAGCTGTTTTTAATCTCTTCTGCAAGTTTGGTGATCCGTGTGGTTTAGTA590.10729223495180942No Hit
CAACAAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGGCTAGTAG590.10729223495180942No Hit
CAACAAGGGCAAGCACTCGGTAGAAGTCACCAACTGCTTTTCGGTGCCACACAATGAGTCAGAA580.10547372249499909No Hit
CAACAAGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.10365521003818876No Hit
CAACAAGGGAACTTGTGTCAGTTCTCTCCTAAGGTAGCTGTCAGAGTTGTTAGTAAAAAGGAGC560.10183669758137844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCA150.004509099858.49446545
GATGCCC150.004509099858.49446545
TGGCGTA150.004509099858.49446545
CCACGAT150.00452561458.4405544
GAAAACG201.9200874E-458.4405544
GCACATT150.00452561458.4405544
CCTAGTC150.00452561458.4405544
ACTAAGG201.9288671E-458.38674541
CTTAGCA201.9288671E-458.38674525
GAACTAA201.9288671E-458.38674539
AACTAAG201.9288671E-458.38674540
CTTTAGC150.00454217358.3867439
CACAAGA150.00454217358.3867426
TCGTGGG150.00454217358.3867426
CCGCACG150.00454217358.3867439
TAGCACA150.00454217358.3867442
GACACCT150.00454217358.3867440
GCACGAT150.00454217358.3867441
CCATATT150.00454217358.3867425
GCCACGA150.00454217358.3867443
Produced by FastQC (version 0.11.5)